3',4',5'-trifluorobiphenyl-2-amine

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: guideline study, GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2007

Report date:

2007

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Details on test material:

Name of test substance: 3',4',5'-Trifluoro-biphenyl-2-ylamine
Batch No.: 33569-66 eing.2
Purity: 99.67 area-%
Homogeneity: Homogeneous
Physical state: Solid
Colour: Dusky pink up to red
Special conditions for storage: Ambient at room temperature

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

The test substance was weighed in the required amounts for the test concentrations directly to the test vessels. Then 180 mL demineralized water were added. The mixtures were stirred for 20 ± 2 hours at room temperature.

Test organisms

Test organisms (species):

activated sludge, domestic

Details on inoculum:

The activated sludge suspension was washed 3 times with tap water and sieved with a fine woven mesh (mesh size about 1 mm). This suspension was adjusted to a concentration of 7.5 g/L dry weight and pre-aerated over night. After the pre-aeration the lost water of the suspension was replaced. An aliquot of this suspension was added to the test vessels to obtain a sludge concentration of 1.5 g/L dry substance

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

180 min

Test conditions

Test temperature:

20 ± 2 °C

pH:

7.1-7.2

Dissolved oxygen:

Oxygen concentration during aeration: > 2.5 mg/L
Oxygen concentration immediately before measurement: > 6.5 mg/L

Nominal and measured concentrations:

Test concentrations (nominal): 1000, 500, 250, 125, 62.5 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer-vessel (nominal volume 250 mL)

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic medium: 8 mL/vessel 100-fold concentrated OECD medium

Reference substance (positive control):

yes

Remarks:

3,5 dichlorophenol

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

180 min

Dose descriptor:

EC10

Effect conc.:

65 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

180 min

Dose descriptor:

other: EC20

Effect conc.:

140 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 3

Duration:

180 min

Dose descriptor:

EC50

Effect conc.:

600 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 4

Duration:

180 min

Dose descriptor:

other: EC80

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Details on results:

The values were given with an accuracy of 2 significant digits. The degree of inhibition was evaluated by Probit analysis according to Finney.