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EC number: 412-540-8 | CAS number: 22471-55-2 ET 344 SP
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 9 November 1992 to 12 November 1992
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Ethyl trans-2,2,6-trimethylcyclohexanecarboxylate
- EC Number:
- 412-540-8
- EC Name:
- Ethyl trans-2,2,6-trimethylcyclohexanecarboxylate
- Cas Number:
- 22471-55-2
- Molecular formula:
- C12 H22 O2
- IUPAC Name:
- ethyl trans-2,2,6-trimethylcyclohexanecarboxylate
- Reference substance name:
- Ethyl cis-2,2,6-trimethylcyclohexane-1-carboxylate
- Molecular formula:
- C12H22O2
- IUPAC Name:
- Ethyl cis-2,2,6-trimethylcyclohexane-1-carboxylate
- Reference substance name:
- Unidentified impurities
- Molecular formula:
- Not specified.
- IUPAC Name:
- Unidentified impurities
- Test material form:
- liquid: viscous
- Details on test material:
- Sample name: ET-344 SP
Constituent 1
Constituent 2
impurity 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix
- Test concentrations with justification for top dose:
- Preliminary test +/- S9 mix: 5 / 20 / 78 / 313 / 1250 / 5000 ug/ plateMutation test - S9 mix: 5 / 10 / 20 / 39 / 78 / 156 / 313 ug/ plate + S9 mix: 10 / 20 / 39 / 78 / 156 / 313 ug/ plate
- Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- 2-nitrofluorene
- sodium azide
- N-ethyl-N-nitro-N-nitrosoguanidine
- other: 2-aminoanthracene
- Details on test system and experimental conditions:
- Bacterial Strains :Salmonella typhimurium TA 100, TA 1535, TA 98, TA 1537 and Esherichia coli WP2 uvrA were obtained from Institute for Fermentation,JAPAN.Cultures of each strain were prepared by incubating an aliquot of parent stock culture in nutrient broth a t 37°C for 18 hours. Test Procedures: (Pre-incubation Method)After mixing 0.1mL of test material solution, 0.5mL of phosphate buffer or S9 Mix and 0.1 mL of culture of tester strain in a test tube, the mixture was incubated a t 37°C for 20 minutes.Then 2 mL of top agar was added in the test tube, the mixture of each tube was poured on minimal agar plate. The plates were incubated at 37 °C for 2 days and scored for revertants.The test was performed in tripilcate at each concentration.
- Statistics:
- No
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- In case of no growth inhibition, there was no significant difference in the number of the colonies between the test compound dosages and the solvent control with any of the bacteria used here.
- Remarks on result:
- other: all strains/cell types tested
Applicant's summary and conclusion
- Conclusions:
- The test results indicated that the compound is not mutagenic under the test system used (with and without metabolic activation).
- Executive summary:
According to OECD TG 471, the mutagenic activity of Ethyl 2,2,6-trimethylcyclohexanecarboxylate provided by TAKASAGO International Corporation was tested by the in vitro direct and metabolic activation assays using Salmonella typhimurium TA 100, TA 1535, TA 98, TA 1537 and Esherichia coli WP2 uvrA.
The Pre-incubation Method was followed.
Vehicule and positive control were included in the study.
The concentrations of test item were as follows:
Preliminary test
+/- S9 mix: 5 / 20 / 78 / 313 / 1250 / 5000 ug/ plate
Mutation test
- S9 mix: 5 / 10 / 20 / 39 / 78 / 156 / 313 ug/ plate
+ S9 mix: 10 / 20 / 39 / 78 / 156 / 313 ug/ plate
The test was performed in tripilcate at each concentration.
In case of no growth inhibition, there was no significant difference in the number of the colonies between the test compound dosages and the solvent control with any of the bacteria used here.
The test results indicated that the compound is not mutagenic under the test system used.
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