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EC number: 221-717-7 | CAS number: 3209-22-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
The acute oral LD50 value in male rats is 1070 mg/kg (Löser 1980) and the dermal LD50 value in rats is greater than 2500 mg/kg bw (Löser 1981). No acute inhalation study is available.
However, the acute oral toxicity study in cats demonstrate a methemoglobin formation. Therefore the classification for acute toxicity is increased.
Key value for chemical safety assessment
Acute toxicity: via oral route
Link to relevant study records
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1980
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable, well-documented study report which meets basic scientific principles.
- Principles of method if other than guideline:
- Acute oral toxicity in rats
- GLP compliance:
- no
- Test type:
- standard acute method
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Winkelmann, Borchen, Germany
- Age at study initiation: 9 weeks
- Weight at study initiation: 177 g
- Housing: 5 animals per cage (Macrolon Type III)
- Diet (e.g. ad libitum): Altromin R 1324 (Altromin GmbH, Lage, Germany) ad libitum
- Water (e.g. ad libitum): tap-water ad libitum
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 1.5
- Humidity (%): 60 +/- 5
- Photoperiod (hrs dark / hrs light): 12/12 - Route of administration:
- oral: gavage
- Vehicle:
- polyethylene glycol
- Details on oral exposure:
- VEHICLE
- Amount of vehicle (if gavage): 20 ml/kg bw - Doses:
- 100, 500, 1000, 1500, 2000, 3100 mg/kg bw
- No. of animals per sex per dose:
- 10
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days.
- Frequency of observations and weighing: Animals were observed 1, 2, 4, 8 and 24 h post-application, and twice daily thereafter up to 14 days. Animals were weighed on the application day and at the end of the observation period (day 14). - Statistics:
- LD50 with confidence interval for p<= 0.05 was calculated by Probit-analysis (Fink and Hund 1965. Arzneim.-Forsch. 15:624).
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 1 070 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- 0.77 - 1.36
- Remarks on result:
- other: clinical signs: reduced body weights, increased diuresis, sedation, scrubby coat
- Mortality:
- refer to remarks on results
- Clinical signs:
- other: refer to remarks on results
- Gross pathology:
- not examined
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- The calculated LD50 was 1070 mg/kg bw.
- Executive summary:
Löser (1980) The acute oral toxicity of 1,2 -dichloro-3 -nitrobenzene was investigated in male Wistar rats. 6 Groups of 10 animals were dosed with 100, 500, 1000, 1500, 2000 and 3100 mg/kg bw 1,2 -dichloro-3 -nitrobenzene per gavage, and observed for 14 days following the exposure for mortality and clinical signs. Mortalities occurred at dose levels equal to and exceeding 500 mg/kg bw between day 2 and 9 following administration. Enhanced diuresis, loss of weight and scubby fur were observed in all animals dosed 500 - 3100 mg/kg bw. These symptoms were slight to moderate and started to appear 1 h (1000 - 3100 mg/kg bw) and on day 2 (500 mg/kg bw) after administration, continuing up to the end of the observation period. No clinical signs were observed in animals dosed with 100 mg/kg bw. The calculated LD50 was 1070 mg/kg bw, with a confidence interval for p < 0.05 = 0.77 - 1.36
Reference
Mortality
Dose level (mg/kg bw) | Mortality |
100 | 0/10 |
500 | 1/10 |
1000 | 4/10 |
1500 | 8/10 |
2000 | 8/10 |
3100 | 10/10 |
Mortalities occurred at dose levels equal to and exceeding 500 mg/kg bw between day 2 and 9 following administration. Enhanced diuresis, loss of weight and scubby fur were observed in all animals dosed 500 - 3100 mg/kg bw. These symptoms were slight to moderate and started to appear 1 h (1000 - 3100 mg/kg bw) and on day 2 (500 mg/kg bw) after administration, continuing up to the end of the observation period. No clinical signs were observed in animals dosed with 100 mg/kg bw.
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed
- Dose descriptor:
- LD50
- Value:
- 1 070 mg/kg bw
- Quality of whole database:
- Acceptable, well-documented study report which meets basic scientific principles.
Acute toxicity: via inhalation route
Endpoint conclusion
- Endpoint conclusion:
- no study available
Acute toxicity: via dermal route
Link to relevant study records
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1981
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable, well-documented study report which meets basic scientific principles.
- Principles of method if other than guideline:
- Acute dermal toxicity in rats
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- yes
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Winkelmann, Borchen, Germany
- Age at study initiation: ca. 14 weeks
- Weight at study initiation: 179 g
- Housing: 5 animals per cage (Macrolon type III)
- Diet (e.g. ad libitum): Altromin R 1324 (Altromin GmbH, Lage, Germany) ad libitum
- Water (e.g. ad libitum): tap-water ad libitum
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 1.5
- Humidity (%): 60 +/- 5
- Photoperiod (hrs dark / hrs light): 12/12 - Type of coverage:
- not specified
- Vehicle:
- polyethylene glycol
- Duration of exposure:
- not specified
- Doses:
- 2500 mg/kg bw
- No. of animals per sex per dose:
- 10 males
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Animals were observed for clinical signs and mortality several times on the day of administration, and twice daily thereafter (once on weekends and holidays) up to the end of the observation period. Animals were weighed on the day of admisitration and at hte end of the 14-days observation period.
- Necropsy of survivors performed: no - Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- > 2 500 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: no mortality, no clinical signs were observed
- Mortality:
- no effects
- Clinical signs:
- other: no effects
- Gross pathology:
- not examined
- Interpretation of results:
- GHS criteria not met
- Executive summary:
The test material was dissolved in Lutrol (polyethylene glycol) at 0.5 g/ml and applied to the skin of 10 male animals. No signs of toxicity were observed in all male animals treated with 2500 mg/kg bw. No mortalities occurred.
Reference
No signs of toxicity were observed in all male animals treated with 2500 mg/kg bw. No mortalities occurred.
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
- Dose descriptor:
- discriminating dose
- Value:
- 2 500 mg/kg bw
- Quality of whole database:
- Acceptable, well-documented study report which meets basic scientific principles.
Additional information
Acute toxicity: oral
The acute oral toxicity of 1,2 -dichloro-3 -nitrobenzene was investigated in male Wistar rats. 6 Groups of 10 animals were dosed with 100, 500, 1000, 1500, 2000 and 3100 mg/kg bw 1,2 -dichloro-3 -nitrobenzene per gavage, and observed for 14 days following the exposure for mortality and clinical signs. Mortalities occurred at dose levels equal to and exceeding 500 mg/kg bw between day 2 and 9 following administration. Enhanced diuresis, loss of weight and scubby fur were observed in all animals dosed 500 - 3100 mg/kg bw. These symptoms were slight to moderate and started to appear 1 h (1000 - 3100 mg/kg bw) and on day 2 (500 mg/kg bw) after administration, continuing up to the end of the observation period. No clinical signs were observed in animals dosed with 100 mg/kg bw. The calculated LD50 was 1070 mg/kg bw (Löser 1980).
Acute toxicity: dermal
The acute dermal toxicity of the test substance 2,3-dichloronitrobenzene was evaluated with male Wistar rats in a limited documented study. The test material was dissolved in Lutrol (polyethylene glycol) at 0.5 g/ml and applied to the skin of 10 male animals. No signs of toxicity were observed in all male animals treated with 2500 mg/kg bw. No mortalities occurred. (Löser 1981).
Justification for classification or non-classification
The acute oral LD50 value in male rats is 1070 mg/kg (Löser 1980) and the dermal LD50 value in rats is greater than 2500 mg/kg bw (Löser 1981). No acute inhalation study is available.
According to CLP classification criteria (Regulation (EC) No 1272/2008) a classification as Acute Tox. 4, H 302 (harmful if swallowed), is adequate.
Based on the the acute oral toxicity study in cats, which demonstrate a methemoglobin formation, the classification is tightened.
Therefore, 1,2-dichlore-3-nitrobenzene is classified as Acute Tox. 3 (H301: Toxic if swallowed), Acute Tox. 3 (H331: Toxic if inhaled), Acute Tox. 4 (H312: Harmful in contact with skin).
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