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EC number: 225-561-0 | CAS number: 4927-36-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- The study was conducted between 13 September 2004 and 29 November 2004.
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- In accordance with GLP conditions, but tested only up to 30%
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 004
- Report date:
- 2004
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
- Deviations:
- yes
- Remarks:
- maximum concentration tested was 30%
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.2600 (Skin Sensitisation)
- GLP compliance:
- yes
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- 5-methyl-5-phenylhexan-3-one
- EC Number:
- 225-561-0
- EC Name:
- 5-methyl-5-phenylhexan-3-one
- Cas Number:
- 4927-36-0
- Molecular formula:
- C13H18O
- IUPAC Name:
- 5-methyl-5-phenylhexan-3-one
- Test material form:
- liquid
Constituent 1
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- other: CBA/J
- Sex:
- female
- Details on test animals and environmental conditions:
- TEST ANIMALS
- Source: Jackson Laboratories, Bar Harbor, ME 04609, US
- Age at study initiation: 11 weeks at start of dosing
- Weight at study initiation: 18 to 26 g at the outset of the study (Day 1)
- Housing: 5 animals/cage (randomly allocated)
- Diet (e.g. ad libitum): ad libitum (Certified Rodent Chow 7012C)
- Water (e.g. ad libitum): ad libitum
- Acclimation period: 6 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21.1-26.7
- Humidity (%): 30-53
- Air changes (per hr): No data
- Photoperiod (hrs dark / hrs light): 12/12
Study design: in vivo (LLNA)
- Vehicle:
- other: Diethyl phthalate/ Ethanol 3:1
- Concentration:
- 7.5%, 15% and 30% (w/v)
- No. of animals per dose:
- 5
- Details on study design:
- MAIN STUDY
EXPERIMENTAL PHASE
Groups of five mice were treated with the test item at concentrations of 7.5%, 15% and 30% w/v in DEP/EtOH. No justification on the choice of vehicle is provided. The doses were chosen on the basis that these are typical concentrations used for fragrances. The mice were treated by daily application of 25 µl of the appropriate concentration of the test item to the dorsal surface of both ears for three consecutive days (Days 1, 2, 3). The test item formulation was administered using an automatic micropipette and spread over the dorsal surface of the ear using the tip of the pipette.
A further group of five mice received the vehicle alone in the same manner. The positive control animals were similarly treated to the test animals except that 25 µl of the positive control item, Hexylcinnamaldehyde [HCA], at a concentration of 35% v/v. On day 6, all mice were injected i.v. with 250 µl of sterile saline containing 20µCi of 3H-thymidine.
OBSERVATIONS
- Clinical Observations: All animals were observed before dosing and once post-dose on Day 1 to 3. After this observations continued on a daily basis on Days 4, 5 and 6. Any signs of toxicity or signs of ill health during the test were recorded. The animals were also examined daily for erythema and edema on the site of application.
- Bodyweights: The bodyweight of each mouse was recorded on Day 1 (prior to dosing) and Day 6 (prior to termination).
TERMINATIOIN
Five hours following the administration of 3HTdR all mice were killed by carbon dioxide asphyxiation. For each individual animal of each group the draining auricular lymph nodes were excised and processed. A single cell suspension of the lymph node cells for each individual animal was prepared. The lymph node cells were rinsed with PBS and precipitated with 5% trichloroacetic acid during night. The lymph node cells suspension was transferred to a centrifuge tube and thereafter, the pellets were resuspended in 1 ml TCA and transferred to scintillation fluid vials for the measurement of the radioactive material. - Positive control substance(s):
- hexyl cinnamic aldehyde (CAS No 101-86-0)
- Statistics:
- Data was processed to give group mean values for disintegrations per minute and standard deviations where appropriate. Increases in the radioactivity compared to the vehicle control were recorded as stimulation indices (SI). Individual DPM values were analyzed with log values. Dunett's test was used to determine significance when required.
Results and discussion
- Positive control results:
- The positive control item, Hexylcinnamaldehyde, gave a Stimulation Index of 7.11 which was statistically significant when compared to the vehicle control group.
In vivo (LLNA)
Resultsopen allclose all
- Key result
- Parameter:
- EC3
- Test group / Remarks:
- All test groups
- Remarks on result:
- other: up to 30% alll SI results are < 3
- Parameter:
- SI
- Value:
- 0.89
- Test group / Remarks:
- 7.5% (w/v)
- Parameter:
- SI
- Value:
- 0.8
- Test group / Remarks:
- 15% (w/v)
- Parameter:
- SI
- Value:
- 1.12
- Test group / Remarks:
- 30% (w/v)
- Cellular proliferation data / Observations:
- CELLULAR PROLIFERATION DATA
Mean DPM (+/- SEM) were:
- Vehicle control: 233 +/- 44
- 7.5%: 207 +/- 39
- 15%: 187 +/- 18
- 30%: 260 +/- 7
DETAILS ON STIMULATION INDEX CALCULATION
Stimulation Index = dpm/lymph node test item treated group / dpm/lymph node vehicle control.
- 7.5%: SI = 0.89
- 15%: SI = 0.8
- 30%: SI = 1.12
EC3 CALCULATION
A dose-response relationship was not observed. A calculation of the EC3 value was not performed because no test concentrations produced a S.I. of 3 or higher.
CLINICAL OBSERVATIONS:
One mouse in the group treated with the test article at 15% was found dead on day 5. A necropsy revealed no gross lesions. All other mice survived and did not show signs of toxicity. The lymph nodes of all animals which were treated with the test item were normal in size and appearance. At termination both ears were measured. There was one statistically significant different in the ear measurement in the group treated with the test article at a dose of 7.5%. This slight decrease was not considered to be biologically significant.
BODY WEIGHTS
Body weight changes of the test animals between Day 1 and Day 6 were comparable to those observed in the corresponding control group animals over the same period.
Applicant's summary and conclusion
- Interpretation of results:
- other: not sensitising
- Remarks:
- based on CLP criteria (EC 1272/2008 and its updates)
- Conclusions:
- Under the conditions of this test, Damascol did not elicit a Stimulation Index of greater than 3 and was therefore not considered to be a sensitiser up to 30%. Therefore, the substance does not need to be classified as skin sensitiser in accordance with the criteria outlined in Annex I of the CLP Regulation (1272/2008/EC and its updates).
- Executive summary:
- The skin sensitisation potential of the test substance has been tested according to OECD TG 429 (Local Lymph Node Assay) and under GLP conditions. The positive control showed a statistically significant induction of the stimulation index as compared to the vehicle control. At doses of 7.5, 15 and 30% (w/v) Damascol, the following Stimulation Index (SI) values were found, respectively: 0.89, 0.80 and 1.12. As these SI values are all below 3 and no EC3 value could be calculated, the substance was not considered to be a skin sensitiser under the conditions of this test. Therefore, the substance does not need to be classified as skin sensitiser up to 30%.
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