Dibenzo[b,f]cyclohepten-1-one

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Authroritative database

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Principles of method if other than guideline:

These tests are conducted based on OECD-GLP standard and OECD test guidelines to study the short term toxicity to aquatic invertebrates by Acute Immobilisation Test.

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material (as cited in study report): 5H-dibenzo[a,d]cyclohepten-5-one
- Molecular formula: C15 H10 O
- Molecular weight: 206.243 g/mol
- Smiles notation: c12c(ccc3c(c1=O)cccc3)cccc2
- Inchl : 1S/C15H10O/c16-15-13-7-3-1-5-11(13)9-10-12-6-2-4-8-14(12)15/h1-10H
- Substance type: Organic
- Physical state: solid

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea

Test type:

not specified

Water media type:

freshwater

Total exposure duration:

48 h

Hardness:

no data

Test temperature:

no data

pH:

no data

Dissolved oxygen:

no data

Salinity:

no data

Nominal and measured concentrations:

no data

Details on test conditions:

no data

Reference substance (positive control):

not specified

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

1.9 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Validity criteria fulfilled:

not specified

Conclusions:

The short term toxicity to aquatic invertebrates test was performed according to the OECD Guideline 202. The EC50 value of dibenzo[b,f]cyclohepten-1-one (2222-33-5) in Daphnia magna was found to be 1.9 mg/l based on immobilisation effect.

Executive summary:

The short term toxicity to aquatic invertebrates test was performed according to the OECD Guideline 202. The test was performed on Daphnia magna for 48 hrs using 5H-dibenzo[b,f]cyclohepten-1-one at 1.9 mg/l concentration. After experiment it was determined that the EC50 value of 5H-dibenzo[b,f]cyclohepten-1-one (2222-33-5) in Daphnia magna was 1.9 mg/l based on immobilisation effect. On the basis of CLP regulation 5H-dibenzo[b,f]cyclohepten-1-one (2222-33-5) was considered likely to be toxic to aquatic invertebrates and can be classified in aquatic chronic 2 category based on CLP regulation.

Description of key information

The short term toxicity to aquatic invertebrates test was performed according to the OECD Guideline 202. The test was performed on Daphnia magna for 48 hrs using dibenzo[b,f]cyclohepten-1-one at 1.9 mg/l concentration. After experiment, the EC50 value of dibenzo[b,f]cyclohepten-1-one (2222-33-5) in Daphnia magna was determined to be 1.9 mg/l based on immobilisation effect.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

1.9 mg/L

Additional information

The study of 5H-dibenzo[b,f]cyclohepten-1-one to aquatic invertebrate was performed which was summaries with one experimental study and prediction of the chemical as follows:

Short term toxicity to aquatic invertebrates was studied for 5H-dibenzo[b,f]cyclohepten-1-one by J-check (Results of Eco-toxicity tests of chemicals conducted by Ministry of the Environment in Japan,2000).The test was performed in Daphnia magna according to the OECD Guideline 202. The time period for treatment was 48 hrs. After the experiment, the EC 50 value for Daphnia magna by 5H-dibenzo[b,f]cyclohepten-1-one was determined to be 1.9 mg/l on the basis of immobilisation effects.

In prediction done by SSS (2017) using the OECD QSAR toolbox version 3.4 with log kow as the primary descriptorand considering the five closest read across substances, the short term toxicity on aquatic invertebrates was predicted for5H-dibenzo[a,d]cyclohepten-5-one (CAS no 2222-33-5).From prediction the EC50 value was estimated to be 132.63mg/L on the basis of intoxication for Daphnia magna for 48 hrs duration.

Thus based on QSAR prediction, 5H-dibenzo[b,f]cyclohepten-1-one was considered to be non hazardous to aquatic invertebrates but as the experimental study from J-check concluded that 5H-dibenzo[b,f]cyclohepten-1-one was likely to be toxic to aquatic invertebrate. Hence, based on the EC 50 value which is 1.9 mg/l, 5H-dibenzo[b,f]cyclohepten-1-one was classified in aquatic chronic 2 according to the CLP regulations.