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Diss Factsheets
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EC number: 212-742-4 | CAS number: 865-49-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- publication
- Title:
- Acute and subchronic nhalation toxicity of chloroform in rats and mice
- Author:
- Kasai T, Nishizawa T, Arito H, Nagano K, Yamamoto S, Matsushima T, Kawamoto T
- Year:
- 2 002
- Bibliographic source:
- Journal of Occupational Health 44, 193-202
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Guidelines No. 412 and 413
- GLP compliance:
- no
- Test type:
- fixed concentration procedure
- Limit test:
- no
Test material
- Reference substance name:
- Chloroform
- EC Number:
- 200-663-8
- EC Name:
- Chloroform
- Cas Number:
- 67-66-3
- Molecular formula:
- CHCl3
- IUPAC Name:
- chloroform
- Test material form:
- liquid
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Fischer 344/DuCrj
- Sex:
- male/female
Administration / exposure
- Route of administration:
- inhalation: vapour
- Type of inhalation exposure:
- whole body
- Vehicle:
- other: unchanged (no vehicle)
- Analytical verification of test atmosphere concentrations:
- not specified
- Duration of exposure:
- 6 h
- Concentrations:
- 500, 1000, 2000, 4000, 8000 ppm (2.44, 4.88, 9.76, 19.52, 39.04 g/m3)
- No. of animals per sex per dose:
- 10 females, 10 males
- Control animals:
- yes
- Details on study design:
- exposure for 5 days/week, during 2 weeks
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- other: NOEC
- Effect level:
- 1 000 ppm
- Exp. duration:
- 14 d
- Remarks on result:
- other: 6 h/day on 5 days/week
- Mortality:
- No mortality occurred at concentrations of 500 or 1000 ppm (see Table 1). All mice died on the first or second day of exposure to 2000 ppm or above.
- Other findings:
- Surviving males and females exposed to 500 and 1000 ppm had vacuolic changes in proximal tubules of the kidneys and in the central area of the liver, in addition to desquamation, atrophy and disarrangement of the olfactory epithelium and oedema of the lamina propria of the nasal cavity.
Any other information on results incl. tables
Table 1: Mortality occurring in the inhalation toxicity study. Numbers in brackets indicate the number of rats dying on specified days.
Exposure concentration |
Rats |
|||||
Male |
Female |
|||||
0 ppm |
0 |
0 |
||||
500 ppm |
0 |
0 |
||||
1000 ppm |
0 |
0 |
||||
2000 ppm |
10 |
(9/1st) |
(1/2nd) |
10 |
(3/1st) |
(2/2nd) |
4000 ppm |
10 |
(9/1st) |
(1/2nd) |
10 |
(9/1st) |
(1/2nd) |
8000 ppm |
10 |
(10/1st) |
10 |
(10/1st) |
Applicant's summary and conclusion
- Conclusions:
- Inhalation exposure of male and female Fisher 344/DuCrj rats to 1000 ppm chloroform vapours during 6 hours/day on 5 days during a period of two weeks did not cause any mortality.
- Executive summary:
The inhalation toxicity of chloroform vapours to female and male Fisher 344/DuCrj rats was tested over a period of two weeks with exposures for 6 hours per day on five days per week. No mortality occurred at exposure levels of 500 or 1000 ppm, whereas all test animals died within 48 hours at exposure levels of 2000 ppm and above. Surviving males and females exposed to 500 and 1000 ppm had vacuolic changes in proximal tubules of the kidneys and in the central area of the liver, in addition to desquamation, atrophy and disarrangement of the olfactory epithelium and oedema of the lamina propria of the nasal cavity.
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