Chromate(2-), [4-[(5-chloro-2-hydroxy-3-nitrophenyl)azo]-2,4-dihydro-5-methyl-2-phenyl-3H-pyrazol-3-onato(2-)][3-[[1-(3-chlorophenyl)-4,5-dihydro-3-methyl-5-oxo-1H-pyrazol-4-yl]azo]-4-hydroxy-5-nitrobenzenesulfonato(3-)]-, disodium

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Experimental Starting Date: 01 December, 2015 : Experimental Completion date: 08 Decemebr, 2015; Study Completion Date:: 22 December, 2015

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

None

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

Principles of method if other than guideline:

None

GLP compliance:

no

Type of method:

flask method

Specific details on test material used for the study:

Identification: FAT 20202/HD TE
Lot: 111
Appearance: Dark red powder

Key result

Water solubility:

10.9 g/L

Conc. based on:

test mat.

Loading of aqueous phase:

10 g/L

Temp.:

20 °C

Remarks on result:

other: pH is not available

Details on results:

None

Preliminary Visual Estimation of the Water Solubility:

Appearance of the test mixtures in the preliminary test

Amount of the test item in mg	Total volume in mL	Appearance of mixture
10	0.1	Undissolved
	0.5	Undissolved
	1	dissolved

The preliminary visual experiments showed that the water solubility of the test item is around 10 g/l.

Flask-Method:

Water solubility of the test item – experiments A to D

Experiment	A	B	C	D(blank)
Amount of test item (mg)	1000	1000	1000	--
Stirring time at 30°C (h)	24	48	72	72
Measured concentration (ng/mL)	11.0	11.2	10.4	-
Dilution	0.01ml/10ml	0.01ml/10ml	0.01ml/10ml	.
Water solubility (μg/L)	11.0	11.2	10.4	-

 

Average experiments A to C: 10.9 g/l

Conclusions:

The water solubility of the test item FAT 20202/H at a temperature of 20 °C was determined to be 10.9 g/l.

Executive summary:

The water solubility of FAT 20202/H was evaluated in a study conducted according to EU Method A.6 and OECD Guideline 105. Flask method was used for this purpose. Three flasks were prepared with about 1000 mg test item and 6 ml water. The batches were stirred for different times at 30 °C (experiment A for 24 h, experiment B for 48 h, experiment C for 72 h) in tightly closed flasks. After equilibration (further stirring for 24 h at 20 °C) a saturated solution with a precipitate of the test item could be observed. Aliquots were taken and centrifuged (30 minutes with 5000 rpm at 20 °C). Subsequently the solution was filtered over 0,20 μm micro filters. The solution is dark colored and was analyzed by VIS-spectroscopy (502 nm).The concentration of the test item was determined by VIS-spectroscopy (502 nm). Another experiment was performed according to this procedure but without test item (“blank”). Based on the findings of the study, the water solubility of FAT 20202/H at 20 °C was determined to be 10.9 g/l.

Description of key information

The water solubility of FAT 20202/H was evaluated in a study conducted according to EU Method A.6 and OECD Guideline 105. Flask method was used for this purpose.

Three flasks were prepared with about 1000 mg test item and 6 ml water. The batches were stirred for different times at 30 °C (experiment A for 24 h, experiment B for 48 h, experiment C for 72 h) in tightly closed flasks. After equilibration (further stirring for 24 h at 20 °C) a saturated solution with a precipitate of the test item could be observed. Aliquots were taken and centrifuged (30 minutes with 5000 rpm at 20 °C). Subsequently the solution was filtered over 0.20 μm micro filters. The solution is dark colored and was analyzed by VIS-spectroscopy (502 nm).The concentration of the test item was determined by VIS-spectroscopy (502 nm). Another experiment was performed according to this procedure but without test item (“blank”).

Based on the findings of the study, the water solubility of FAT 20202/H at 20 °C was determined to be 10.9 g/l.

Key value for chemical safety assessment

Water solubility:

10.9 g/L

at the temperature of:

20 °C