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EC number: 207-803-7 | CAS number: 495-54-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 21-03-2017 - 25-03-2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental test result performed using standard test guidelines
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- no
- Specific details on test material used for the study:
- Test Item Name 4-(phenylazo)benzene-1,3-diamine
CAS No. 495-54-5
Batch No. KCP/FS/43/17
Physical Appearance orange powder
Date of manufacturing 08-01-17
Date of expiry 08-12-17
Purity not available in coa
Stability Stable in recommended storage conditions
Storage Conditions 1. Ambient Temp (23 to 27 °C)
Precautions Wear respiratory protection. Avoid contact with skin and eyes. Avoid dust formation. Avoid breathing dust, fume, vapours, mist, spray or gas. Ensure adequate ventilation. Evacuate personnel to safe areas. Use only in a chemical fume hood.
Solubility in Water 348.393 mg/L - Analytical monitoring:
- not specified
- Vehicle:
- yes
- Remarks:
- water
- Details on test solutions:
- Preparation of the test Solutions : The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with 2 hours continuous stirring for achieving test concentrations of 0.0625mg/L,0.125mg/L,0.25mg/L,0.5mg/L,1mg/L ,respectively.
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name:Zebra Fish
- Strain:-
- Source:Koyal aquarium, Nagpur
- Age at study initiation (mean and range, SD):3-4 months
- Length at study initiation (length definition, mean, range and SD):average 1.79 cm
- Weight at study initiation (mean and range, SD):0.1204g (average)
- Method of breeding: -
- Feeding during test-
- Food type:Standard brand feed,
- Amount:-
- Frequency: once daily - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 148.5 mg of CaCO3
- Test temperature:
- 23°C
- pH:
- 7.03
- Dissolved oxygen:
- 7.8 mg/l
- Nominal and measured concentrations:
- 0.0625mg/L,0.125mg/L,0.25mg/L,0.5mg/L,1mg/L
- Details on test conditions:
- Photoperiod: 16 hours light and 8 hours dark
Volume of water: 2 liters
No. of animals per vessel: 8 fishes
Test temperature: 23 °C
Adaptation: Test conditions corresponded to housing conditions
Test dates: Test started at : 13-03-2017
Test Exposure date: 21-03-2017
Date of experimental termination : 25-03-2017
Withdrawal of food: 1 day before and during exposure
Aeration: Aeration in test vessels was provided 1 day before the start of experiment - Reference substance (positive control):
- not specified
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- no mortality was observed
- Sublethal observations / clinical signs:
Mortality and symptoms:
Nominal
Concentration
(mg/l)
Number of
fish
Mortality (cumulated) after
3 h
6 h
24 h
48 h
72 h
96 h
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
-
-
-
-
-
-
-
-
-
-
-
-
0.0625
4
4
-
-
-
-
-
-
-
-
-
-
-
-
0.125
4
4
-
-
-
-
-
-
-
-
-
-
-
0.25
4
4
-
-
-
-
-
-
-
-
-
-
-
-
0.5
4
4
-
-
-
-
-
-
-
-
-
-
-
-
1
4
4
-
-
-
-
-
-
-
-
-
-
-
-
Visible Symptoms:
Nominal
Concentration
(mg/l)
Number of
fish
Symptom (cumulated) after
3 h
6 h
24 h
48 h
72 h
96 h
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
0.0625
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
0.125
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
0.25
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
0.5
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
1
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
Explanation of symptoms:
A: apathy
N: Narcotic-like state
C: Swimming near water surface
M: Slow movements compared to control
NS: No abnormal symptoms
S: Surfacing behaviour
L: Loss of equilibrium
SM: Shimming movement
H1: Hyperactivity
H2: Hypo activity
B: Bending behavior
F: Fin rot
IS: Invert Swimming
SB: Swimming behaviour
RF: Respiratory Function
P: Pigmentation
pH and oxygen content:
Nominal
Concentration
(mg/l)
Number of
fish
pH readings after
24 h
48 h
72 h
96 h
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
8.04
8.04
8.12
8.15
8.23
8.25
8.32
8.35
0.0625
4
4
7.17
7.15
8.00
8.01
8.13
8.13
8.30
8.31
0.125
4
4
7.19
7.20
8.05
8.05
8.17
8.18
8.35
8.36
0.25
4
4
7.23
7.24
8.09
8.09
8.21
8.20
8.39
8.39
0.5
4
4
7.25
7.26
8.12
8.13
8.24
8.24
8.40
8.41
1
4
4
7.31
7.35
8.17
8.19
8.26
8.28
8.42
8.45
Nominal
Concentration
(mg/l)
Number of
fish
Oxygen content (mg/L) after
24 h
48 h
72 h
96 h
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
7.22
7.21
7.01
7.01
6.90
6.90
6.80
6.82
0.0625
4
4
7.12
7.11
7.13
7.13
7.00
7.00
7.20
7.21
0.125
4
4
6.93
6.88
7.09
7.09
6.99
6.99
7.19
7.15
0.25
4
4
6.91
6.92
7.07
7.07
6.90
6.91
7.21
7.23
0.5
4
4
6.89
6.89
7.05
7.05
7.01
7.01
7.00
7.01
1
4
4
6.99
6.99
7.01
7.01
7.02
7.02
7.03
7.01
Test temperature:
Nominal
Concentration
(mg/l)
Number of
fish
Temperature (°C) after
24 h
48 h
72 h
96 h
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
22.9
22.9
22.5
22.5
23.2
23.2
22.9
22.8
0.0625
4
4
23.1
23.1
22.5
22.5
23.2
23.2
22.9
22.9
0.125
4
4
23.2
23.2
22.5
22.5
23.2
23.2
22.9
22.9
0.25
4
4
23.2
23.2
22.5
22.5
23.2
23.2
22.9
22.9
0.5
4
4
23.2
23.2
22.5
22.5
23.2
23.2
22.9
22.9
1
4
4
23.1
23.1
22.5
22.5
23.2
23.2
22.9
22.9
- Validity criteria fulfilled:
- yes
- Conclusions:
- A 96 h study was conducted for the substance 4-(phenylazo)benzene-1,3-diamine CAS No. 495-54-5 on Zebra fish(Danio rerio), at a test concentrations of 0.0625mg/L, 0.125mg/L, 0.25mg/L, 0.5mg/L, 1mg/L . The fishes were moving slowly as compared to control. No mortalities were found in the control aquaria. The lethal concentrations LC50 for 4-(phenylazo)benzene-1,3-diamine was found to be >1 mg/L.
- Executive summary:
Fish Acute Toxicity test according to OECD Guideline 203 was conducted for 4-(phenylazo)benzene-1,3 -diamine The nominal concentration selected for the experiment were 0.0625mg/L,0.125mg/L,0.25mg/L,0.5mg/L,1mg/Land test fish were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be >1 mg/L.
LC0 (96 hours) (highest loading at which no mortality was observed) = 1 mg/L
LC50 (96 hours) Experimental = >1 mg/L
LC100 (96 hours) (lowest loading at which 100% mortality was observed) = No mortality was observed
Reference
Description of key information
A 96 h study was conducted for the substance 4-(phenylazo)benzene-1,3-diamine CAS No. 495-54-5 on Zebra fish(Danio rerio), at a test concentrations of 0.0625mg/L, 0.125mg/L, 0.25mg/L, 0.5mg/L, 1mg/L . The fishes were moving slowly as compared to control. No mortalities were found in the control aquaria. The lethal concentrations LC50 for 4-(phenylazo)benzene-1,3-diamine was found to be >1 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 1 mg/L
Additional information
Different experimental studies of the substance 4-(phenylazo)benzene-1,3-diamine were reviewed for Short term toxicity to fish endpoint and are summarised as follows:
Fish Acute Toxicity test according to OECD Guideline 203 was conducted for 4-(phenylazo)benzene-1,3 -diamine The nominal concentration selected for the experiment were 0.0625mg/L,0.125mg/L,0.25mg/L,0.5mg/L,1mg/Land test fish were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be >1 mg/L.
LC0 (96 hours) (highest loading at which no mortality was observed) = 1 mg/L
LC50 (96 hours) Experimental = >1 mg/L
LC100 (96 hours) (lowest loading at which 100% mortality was observed) = No mortality was observed.
From peer reviewed journal (Yasuhide Tonogai et al;The Journal of Toxicological Sciences,1982) Short term toxicity to fish was performed for the test material 2,4 Diaminoazobenzene using test organism Oryzias latipes. The test material was dissolved in 1 mL of ethanol and then diluted with water at a concentration of 0.5 mg/L (500 µg/L)and 0.3 mg/L (300 µg/L) for the 24hrs and 48 hrs study period respectively. The LC50 for the test material 2,4 Diaminoazobenzene is 0.5 mg/L for 24 hrs duration and 0.3 mg/L for 48 hrs duration.
Considering the above data, the LC50 value of the substance for fish is observed to be 0.3 - >1 mg/L. This result is not conclusive for classiying a chemical, but considering the harmonised classification of the substance and for the purpose of classification and chemical safety assessment the substance 4-(phenylazo)benzene-1,3 -diamine is considered to classified as Aquatic acute 1 and aquatic chronic 1 category.
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