Reaction mass of 1,2-Diacetoxy-3-butene and 1,2-Dihydroxy-3-butene, monoacetate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

445-630-0

EC Name:

-

Molecular formula:

Unspecified

IUPAC Name:

1-(acetyloxy)but-3-en-2-yl acetate; 2-hydroxybut-3-en-1-yl acetate

Details on test material:

- Test substance: Vinylglykoldicatet ca. 75% ig
- batch No.: Kanister 1,2,4 und 5

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

- Test organisms: Daphnia magna Straus, originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age of test organisms: 2 - 24 h

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

19.6 - 20.3

pH:

7.9 - 8.1

Dissolved oxygen:

6.6 - 8.8 mg/l

Details on test conditions:

Test conditions:
-Culture and test medium: according to the OECD test guideline 202 (M4 medium)
- Test duration: 48 h
- Test temperature: 19.6 - 20.3 °C
- Oxygen content during the test: 6.6 - 8.8 mg/l
- pH-value during the test: 7.9 - 8.1
- Exposure vessels: Glass test tubes sealed with glass plugs (nominal volume 50 ml)
- Test volume: 50 ml
- Light conditions: 16:8 hours, diffuse light (about 1 - 8 microeinstein/m*m*s) at 400 - 750 nm wave length
- Number of replicates: 4
- Individuals/replicate: 5
- Observation time: 0, 24, 48 h
- Observations: swimming ability
- Preparation of stock solution: All test concentrations were weighed in separately in M4 medium by adding 100.3, 50.4, 25.1, 12.3 and 6.4 mg test substance into 1000 ml culture medium. The concentrations were stirred for approx. 5 min at 20 +/- 2 °C.
- Test concentrations: ranging from 6.25 to 100 mg/l, using a spacing factor of 2.0. A control, containing culture medium only was tested in parallel.
- Analytical dose verification: Samples from each test concentration and the control were taken at the start and the end of the experiment. - Statistical evaluation: Probit analysis of the EC50 value. The NOEC, LOEC and the EC100 were determined directyl from the raw data.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

31.6 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

26.8 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

64.8 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

RS-Freetext:
No. of immobile daphnids after x hours in %:
Nominal test concentration (mg/l) / 0 / 24 / 48
Control /   0 /   0 /   0 
6.25    /   0 /   0 /   0 
12.5    /   0 /   0 /   0 
25.0    /   0 /   0 /   0 
50.0    /   0 /   0 /   5 
100     /   0 /  75 / 100 

24 hour ECx values:
EC0: 50 mg/l
EC50: > 50 - < 100 mg/l
EC100: > 100 mg/l

Applicant's summary and conclusion

Validity criteria fulfilled:

yes