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EC number: 276-558-6 | CAS number: 72275-69-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- other: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- From August 04, 2015 to August 07, 2015
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- No data
- Vehicle:
- not specified
- Details on test solutions:
- M4 medium
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Source: RCC Ltd.
- Age at study initiation (mean and range, SD): Young Daphnids, aged less than ‘24’ hours old - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- Not available
- Hardness:
- 220 mg/l
- Test temperature:
- 20.4 °C
- pH:
- 7.59 - 7.70
- Dissolved oxygen:
- 7.43 – 7.66 mg/l
- Salinity:
- Not available
- Nominal and measured concentrations:
- A range finding experiment was conducted to determine the range of concentrations for the main experiment. In range finding experiment, the daphnids were exposed to a series of widely spaced concentrations of the test item viz., 0.001, 0.01, 0.1, 1, 10, 50 and 100 mg/l.
Based on the results of the range finding experiment, seven test concentrations viz., 1, 1.5, 2.3, 3.4, 5.1, 7.6 and 11.4 mg/L were fixed in a geometric series with a separation factor of 1.5. - Details on test conditions:
- TEST SYSTEM
- No. of organisms per vessel: 10 daphnids - Reference substance (positive control):
- yes
- Remarks:
- Potassium Dichromate Pure
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 3.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 6.11 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 5.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- Based on the test results, the EC50 in Daphnia magna observed for a period of 48 hour was calculated as 6.11 mg/L with upper confidence limits of 6.63 mg/L and lower confidence limits of 5.64 mg/L and regression equation Y = -1.3294 + 8.0509 log (x).
The no observed effect concentration (NOEC) was calculated as 3.4 mg/L and the low observed effect concentration (LOEC) was calculated as 5.1 mg/L. - Results with reference substance (positive control):
- Based on the test results, the EC50 of Potassium Dichromate Pure in Daphnia magna observed for a period of ‘24’ hour was calculated as 1.72 mg/L with upper confidence limits of 2.12 mg/L and lower confident limits of 1.40 mg/L and regression equation Y = 4.0957 + 3.8604 log (x). At ‘48’ hour, the EC50 was calculated as 1.33 mg/L with upper confidence limits of 1.63 mg/L and lower confidence limits of 1.08 mg/L and regression equation Y = 4.5239 + 3.8769 log (x).
- Reported statistics and error estimates:
- None
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the test results, the EC50 of test item in Daphnia magna observed for a period of 48 hours was calculated as 6.11 mg/l with upper confidence limits of 6.63 mg/l and lower confidence limits of 5.64 mg/l and regression equation Y = -1.3294 + 8.0509 log (x).
The no observed effect concentration (NOEC) was calculated as 3.4 mg/l and the low observed effect concentration (LOEC) was calculated as 5.1 mg/l. - Executive summary:
Acute Immobilization Toxicity Study in Daphnia magna was conducted in accordance with OECD guideline 202 with test substance. Test item was soluble and hence formulated in M4 medium. Based on the results of the range finding experiment, main experiment was conducted with a range of concentrations viz., 1, 1.5, 2.3, 3.4, 5.1, 7.6 and 11.4 mg/L.
No immobilization was observed in control and daphnids exposed to 1, 1.5 and 2.3 mg/l test concentrations throughout the experimental period. At the end of 24 hour, no immobility was observed in daphnids exposed to any of the test concentrations. Thus, the percent immobilisation at the end of 24 hour was recorded to be 0% in control and daphnids exposed to all the test concentrations. At the end of 48 hour, 2/40, 8/40, 31/40 and 40/40 immobility was recorded in the daphnids exposed to 3.4, 5.1, 7.6 and 11.4 mg/l concentration respectively. Thus the percent cumulative immobilisation at the end of 48 hour was recorded to be 0% in control and daphnids exposed to 1, 1.5 and 2.3 mg/L test concentration whereas, 5%, 20%, 77.5% and 100% was observed in daphnids exposed to 3.4, 5.1, 7.6 and 11.4 mg/L concentrations respectively.
Based on the test results, the EC50 of Similar substance 01 in Daphnia magna observed for a period of 48 hour was calculated as 6.11 mg/L. The no observed effect concentration (NOEC) was calculated as 3.4 mg/L and the low observed effect concentration (LOEC) was calculated as 5.1 mg/L
Reference
Description of key information
EC50 = 6.11 mg/l
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 6.11 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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