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EC number: 256-851-5 | CAS number: 50925-42-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1990
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods
- Qualifier:
- according to guideline
- Guideline:
- other: UBA test protocol: Letale Wirkung beim Zebrabaerbling Brachydanio rerio, (LC 0, LC 50, LC 100; 48 – 96H) Stand Mai 1984
- Version / remarks:
- Stand Mai 1984
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: direct weighing - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: zebra fish
- Source: West Aquarium GmbH (Germany)
ACCLIMATION
- Acclimation conditions: same as test
- Health during acclimation (any mortality observed): mortadility < 5 %
- Medication; none
FEEDING DURING TEST
- no feeding during the exposure period - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 13 ° dH
- Test temperature:
- The temperature varied between 20.8 - 21.6 °C (1990-10-09)
The temperature varied between 21.5 - 22.8 °C (1990-10-16) - pH:
- pH-values varied betweeen 6.6 - 7.6 (1990-10-09)
pH-values varied betweeen 7.2 - 8.1 (1990-10-16) - Dissolved oxygen:
- Oxygen concentration varied between 7.6 - 9.0 mg/L (1990-10-09)
Oxygen concentration varied between 7.6 - 9.0 mg/L (1990-10-16) - Salinity:
- no data
- Conductivity:
- no data
- Nominal and measured concentrations:
- 10, 100, 316 and 1000 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass aquaria holding 5 L of test media covered by glass plates
- Type (delete if not applicable):closed
- Material, size, headspace, fill volume:
- Aeration: gentle aeration
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Freshwater prepared after ISO: acidity: 0.1 +/- 0.02 mmol/L; Ca / Mg: 4:1; pH: 8.0 – 8.4; oxygen: >= 90 % of saturation.
OTHER TEST CONDITIONS
- Photoperiod: 16 h light: 8 h dark
- The criterion of death used in this study was the absence of response to physical stimulation. In addition to observations on mortality at 0, 24, 48, 72 and 96 hours, type and incidence of sub-lethal effects compared with control fish were observed
TEST CONCENTRATIONS
- Range finding study: 10, 100 and 1000 mg/L. - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Remarks:
- geometric mean LC0/LC100
- Effect conc.:
- > 526 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Validity criteria fulfilled:
- yes
- Remarks:
- mortality in the controls did not exceed 10 %, dissolved oxygen concentration remained above 60 % of the air-saturation value throughout the exposure period, pH did not vary by more than 1 unit
- Conclusions:
- After 96 hours the following values were determined: LC 0: 316 mg/L, LC 100: 1000 mg/L, Geometric Meen: 562 mg/L.
- Executive summary:
In accordance with UBA test protocol: Letale Wirkung beim Zebrabaerbling Brachydanio rerio, (LC 0, LC 50, LC 100; 48 – 96H) Stand Mai 1984 a study was performed to assess the acute toxicity of Bayscript Gelb BR to Danio rerio under static conditions.
Groups of ten fish were exposed to a range of concentrations, nominally 10, 100, 316 and 1000 mg/L of Bayscript Gelb BR dissolved in dilution water. Observations were made on the numbers of dead fish and the incidence of sub-lethal effects after 0, 24, 48, 72 and 96 hours of exposure. During the test a temperature range of 20 - 24 °C was maintained in the test vessels, with a maximum temperature fluctuation of +/- 1 °C in the test. The temperature, the pH and the oxygen values were measured at the beginning of the test and every 24 hours thereafter.
After 96 hours the following values were determined: LC 0: 316 mg/L, LC 100: 1000 mg/L, Geometric Meen: 562 mg/L.
Reference
Description of key information
After 96 hours the following values were determined: LC 0: 316 mg/L, LC 100: 1000 mg/L, Geometric Meen: 562 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 562 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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