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EC number: 260-480-4 | CAS number: 56966-52-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP-guideline study with analytical monitoring
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 5-chloro-2-(2,4-dichlorophenoxy)aniline
- EC Number:
- 260-480-4
- EC Name:
- 5-chloro-2-(2,4-dichlorophenoxy)aniline
- Cas Number:
- 56966-52-0
- Molecular formula:
- C12H8Cl3NO
- IUPAC Name:
- 5-chloro-2-(2,4-dichlorophenoxy)aniline
- Details on test material:
- - Name of test material (as cited in study report): FAT 80023/G
- Physical state: solid
- Analytical purity: 100%
- Batch No.: ICMD CH 2.23
- Storage condition of test material: room temperature
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: 3 g test substance and 15 mg vehicle were disolved in 200 ml dechlorinated tap water and were heated for 15 min by 50 °C. After that the solution was homogenized for 15 min and added up to 1 L.
- Chemical name of vehicle: Tween 80
- Concentration of vehicle in test medium: 1.1 mg/L in the highest test concentration
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: zebra fish
- Source: West Aquarium, Bad Lauterberg, Germany
- Age at study initiation: 122 days
- Length at study initiation (mean): 31 mm
- Weight at study initiation (mean): 0.29 g
ACCLIMATION
- Acclimation period: 33 days
- Acclimation conditions: dechlorinated tap water
- Medical treatment: none
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 161 mg/L CaCO3
- Test temperature:
- 22.9 ± 0.4 °C
- pH:
- 7.9 - 8.5
- Dissolved oxygen:
- 89 - 98%
- Nominal and measured concentrations:
- Nominal test concentrations: control, 1.9, 4.3, 9.4, 21, 45, 100, 220 mg/L
Measured test concentrations: < 0.7, n.m., n.m., n.m., n.m., 1.7, 1.4, 1.9 mg/L
n.m. = not measured - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, total volume, test volume: glass aquaria, 5 L, approx. 3 L
- Aeration: slowly aerated
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water
- Intervals of water quality measurement: Temperature, pH, oxygen content after 0, 24, 48, 72, 96 h
OTHER TEST CONDITIONS
- Photoperiod: 12 hours daily
- Light intensity: yellow light
EFFECT PARAMETERS MEASURED: symptoms, mortality after 24, 48, 72, 96 hours
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 1.4 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 1.6 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 1.9 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 1.4 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Details on results:
- - Nominal effect concentrations (96 h):
- LC0 = 100 mg/L
- LC50 = 148 mg/L
- LC100 = 220 mg/L
- Behavioural abnormalities: swimming behaviour (slight - severe symptoms at the treatment groups of 45, 100, 220 mg/L over the whole study period
- Mortality of control: none
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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