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Diss Factsheets
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EC number: 443-090-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From October 17, 2001 to November 14, 2001
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- Type: mixed population of aquatic microorganisms (activated sludge)
Origin: aeration tank of a waste water plant treating predominantly domestic sewage (Kläranlage Odenthal)
Date of collection: October 15, 2001
Pretreatment: none
Concentration of inoculums: 30 mg/L SS - Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- PRETREATMENT OF THE TEST SUBSTANCE
25 mg test substance was weighed out on aluminium foil. This substance inclusive of the aluminium foil was added to the test vessels to give a test concentration of 100 mg/L test substance.
EXPOSURE CONDITIONS
Test volume: 250 mL
Test apparatus: Voith Sapromat
Type of system: closed
Mixing: one magnetic stirrer per test vessel
Incubation time: 28 d
Incubation temperature: 20±1°C
CHEMICAL ANALYSIS
Nitrate-N/Nitrite-N method: Determination of nitrite nitrogen and nitrate nitrogen and the sum of both by flow analysis (CFA and FIA) and spectrometric detection. The nitrate and nitrite concentration at 0 h was determined from a flask with test substance. Therefore an additional determination of nitrate and nitrite of the blank inoculum was not necessary.
Standard: EN ISO 13395
Test apparatus: Continuous Flow Analyser SKALAR SAN Plus System - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 99% pure
- Test performance:
- The used concentrations of the test substance did not show toxic effects to bacteria.
- Parameter:
- other: Biochemical oxygen demand
- Value:
- 8
- Sampling time:
- 28 d
- Remarks on result:
- other: The consumption of oxygen was determined by measuring the quantity of oxygen (produced electrolytically) required to maintain constant gas volume in the respirometer flask.
- Details on results:
- pH of the test vessel: 7.5
Degradation of test substance:
2% degradation after 2 d
4% degradation after 6 d
5% degradation after 8 d
8% degradation after 12 d
8% degradation after 14 d
8% degradation after 16 d
9% degradation after 20 d
11% degradation after 22 d
13% degradation after 26 d
8% degradation after 28 d - Results with reference substance:
- Degradation of reference substance:
56% degradation after 2 d
76% degradation after 6 d
82% degradation after 8 d
89% degradation after 12 d
90% degradation after 14 d
91% degradation after 16 d
92% degradation after 20 d
93% degradation after 22 d
94% degradation after 26 d
94% degradation after 28 d - Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: not readily biodegradable
- Conclusions:
- Under the study conditions, the test substance was considered to be 'not readily biodegradable' in the closed bottle test, with 8% biodegradation at 28 d.
- Executive summary:
A study was conducted to assess the ready biodegradability of the test substance according to the EU Method C.4 -D (which is in most parts equivalent to OECD Guideline 301 F), in compliance with GLP.
100 mg/L of the test substance was inoculated in triplicates with non-adapted activated sludge in a closed flask at a constant temperature (20 ± 1 °C) for up to 28 d. Degradation was assessed by the determination of Biochemical Oxygen Demand (BOD) on Days 2, 6, 8, 12, 14, 16, 20, 22, 26 and 28. A toxicity test and a control solution containing the reference substance benzoic acid, sodium salt (100 mg/L) together with inoculum were used for validation purposes. The test substance degraded 2, 4, 5, 8, 8, 8, 9, 11, 13 and 8% on Days 2, 6, 8, 12, 14, 16, 20, 22, 26 and 28, respectively. No toxicity of the test substance was observed in the toxicity control. The reference compound showed 90% degradation at Day 14.
Hence, under the study conditions, the test substance was considered to be ‘not readily biodegradable’.
Reference
All validity criteria of the test method were met:
-The reference compound reached the level for ready biodegradability by Day 14.
-No toxicity of the test substance was observed in the toxicity control group.
-The difference of extremes of replicate values of the removal of test chemical at the end of the test was less than 20%.
-The oxygen uptake of the inoculums blank was ≤60 mg/L.
-The pH was within the range of 6.5- 8.5 in the test vessels at the end of the test (applicable in case where degradation is less than 60%).Description of key information
Under the study conditions, the test substance was considered to be 'not readily biodegradable' in the closed bottle test, with 8% biodegradation at 28 d.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
A study was conducted to assess the ready biodegradability of the test substance according to the EU Method C.4 -D (which is in most parts equivalent to OECD Guideline 301 F), in compliance with GLP. 100 mg/L of the test substance was inoculated in triplicates withnon-adapted activated sludge ina closed flask at a constant temperature (20 ± 1 °C) for up to28 d. Degradation was assessed by the determination of Biochemical Oxygen Demand (BOD) on Days 2, 6, 8, 12, 14, 16, 20, 22, 26 and 28. A toxicity test and a control solution containing the reference substance benzoic acid, sodium salt (100 mg/L) together with inoculum were used for validation purposes. The test substance degraded 2, 4, 5, 8, 8, 8, 9, 11, 13 and 8% on Days 2, 6, 8, 12, 14, 16, 20, 22, 26 and 28, respectively. No toxicity of the test substance was observed in the toxicity control. The reference compound showed 90% degradation at Day 14. Hence, under the study conditions, the test substance was considered to be ‘not readily biodegradable’.
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