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EC number: 211-219-8 | CAS number: 634-93-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 12/10/2015 - 24/10/2015
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Experimental test result performed using standard OECD test guidelines
- Justification for type of information:
- Experimental test result performed using standard OECD test guidelines
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Principles of method if other than guideline:
- This study was designed to assess the toxic effects of the test compound on the Zebra Fish. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes each. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203.
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): 2,4,6-trichloroaniline
- Molecular formula (if other than submission substance): C6-H4-Cl3-N
- Molecular weight (if other than submission substance): 196.464
- Smiles notation (if other than submission substance): c1(c(cc(Cl)cc1Cl)Cl)N
- InChl (if other than submission substance): 1S/C6H4Cl3N/c7-3-1-4(8)6(10)5(9)2-3/h1-2H,10H2
- Substance type: organic - Analytical monitoring:
- not specified
- Vehicle:
- yes
- Details on test solutions:
- The test substance was slightly soluble in water. Therefore, the stock solution was prepared by dissolving 1gm of the test substance in 1 liters of potable water (passed through reverse osmosis system) and kept it for 30min of sonication. After the completion of the sonication process, the sample was run through HPLC system for getting the actual water solubility of the test substance. The actual solubility obtained was 48.89mg/L. From this stock solution further test concentrations were prepared for achieving test concentrations of 0.8 mg/L, 1.76 mg/L, 3.87mg/L, 8.5mg/L, 18.72mg/L, respectively.
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Asim Fancy Fish Aquarium, Dharampeth, Nagpur, MS (India)
- Age at study initiation (mean and range, SD): no data
- Length at study initiation (length definition, mean, range and SD):average 2.45 cm
- Weight at study initiation (mean and range, SD):0.149g (approx.)
- Method of breeding:
- Feeding during test
- Food type: Standard brand feed
- Amount: No data
- Frequency: once daily - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 75 mg of CaCO3
- Test temperature:
- 21°C
- pH:
- 7.8-8.2
- Dissolved oxygen:
- 7.8-8.3 mg/l
- Nominal and measured concentrations:
- 0.8 mg/L, 1.76 mg/L, 3.87mg/L, 8.5mg/L, 18.72mg/L, respectively
- Details on test conditions:
- - Test vessel: plastic aquaria
- Material, size, headspace, fill volume: water capacity of 5 liters
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- Renewal rate of test solution (frequency/flow rate):
- No. of organisms per vessel: 8 fishes
- No. of vessels per concentration (replicates):
- No. of vessels per control (replicates):
- No. of vessels per vehicle control (replicates):
- Biomass loading rate:
OTHER TEST CONDITIONS
- Adjustment of pH: 7.4
- Photoperiod: 12 hours light and 12-hour darkness
- TDS (ppm): 382
- Conductivity (µScm-1): 1130 - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 8.51 - < 18.72 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Experimental value
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- morphology
- Remarks on result:
- other: through probit analysis
- Details on results:
- LC0 (96 hours) (highest loading at which no mortality was observed) = 8.51mg/L
LC50 (96 hours) Experimental = >8.51mg/L & <18.72mg/L
LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 18.72mg/L
LC50 (through probit analysis)= 10 mg/L (after rounding off) - Sublethal observations / clinical signs:
Mortality and symptoms
Nominal Concentration
(mg/l)
Number of fish
Mortality (cumulated) after
3 h
6 h
24h
48h
72h
96h
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
0 (control)
4
4
-
-
-
-
-
-
-
-
-
-
-
-
0.8 mg/L
4
4
-
-
-
-
-
-
-
-
-
-
-
-
1.76 mg/L
4
4
-
-
-
-
-
-
-
-
-
-
-
-
3.87 mg/L
4
4
-
-
-
-
-
-
-
-
-
-
-
-
8.51mg/L
4
4
-
-
-
-
2
-
-
-
-
-
-
-
18.72 mg/L
4
4
2
3
1
-
1
1
-
-
-
-
-
-
Visible Symptoms:
Nominal Concentration
(mg/l)
Number of fish
Mortality (cumulated) after
3 h
6 h
24h
48h
72h
96h
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
0 (control)
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
0.8 mg/L
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
1.76 mg/L
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
3.87 mg/L
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
8.51mg/L
4
4
IS4
IS4
IS4
IS4
IS2
IS4
NS2
NS4
NS2
NS4
NS2
NS4
18.72 mg/L
4
4
IS2
IS1
IS2
IS1
IS2
IS1
-
-
-
-
-
-
Explanation of symptoms:
A:apathy
N:Narcotic-like state
C:Swimming near water surface
M: Slow movements compared to control
NS:No abnormal symptoms
S:Surfacing behaviour
L:Loss of equilibrium
SM:Shimming movement
H1:Hyperactivity
H2:Hypo activity
B:Bending behavior
F:Fin rot
IS:Invert Swimming
SB:Swimming behaviour
RF:Respiratory Function
P:Pigmentation
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No. 634-93-5 on Zebra fish (Denio rario) was determine to be >8.51mg/L & <18.72mg/L as experimental result and through probit analysis it was observed to be 10 mg/L.
- Executive summary:
Study was conducted to access the effect of test chemical 2,4,6-trichloroaniline on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The nominal concentration selected for the experiment were 0.8 mg/L, 1.76 mg/L, 3.87mg/L, 8.5mg/L, 18.72mg/L, respectively and Zebra Fish Danio rerio were exposed to these concentration for 96 hours. The test substance was slightly soluble in water. Therefore, the stock solution was prepared by dissolving 1gm of the test substance in 1 liters of potable water (passed through reverse osmosis system) and kept it for 30min of sonication. After the completion of the sonication process, the sample was run through HPLC system for getting the actual water solubility of the test substance. The actual solubility obtained was 48.89 mg/L. From this stock solution further test concentrations were prepared for achieving test concentrations of 0.8 mg/L, 1.76 mg/L, 3.87mg/L, 8.5mg/L, 18.72mg/L, respectively.
Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203.
The fishes were moving slowly as compared to control. No mortalities were observed in the control aquaria.
LC0 (96 hours) (highest loading at which no mortality was observed) = 8.51mg/L
LC50 (96 hours) Experimental = >8.51mg/L & <18.72mg/L
LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 18.72mg/L
LC50 (through probit analysis) = 10.06mg/L
After rounding-off the numerical values, the LC 50 was determine to be 10 mg/L.
Based on the LC50 value (10 mg/l), chemical 2,4,6-trichloroaniline, consider to be toxic and classified as aquatic chronic 2 as per the CLP classification criteria.
Reference
Description of key information
Study was conducted to access the effect of test chemical 2,4,6-trichloroaniline on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The nominal concentration selected for the experiment were 0.8 mg/L, 1.76 mg/L, 3.87mg/L, 8.5mg/L, 18.72mg/L, respectively and Zebra FishDanio reriowere exposed to these concentration for 96 hours. The test substance was slightly soluble in water. Therefore, the stock solution was prepared by dissolving 1gm of the test substance in 1 liters of potable water (passed through reverse osmosis system) and kept it for 30min of sonication. After the completion of the sonication process, the sample was run through HPLC system for getting the actual water solubility of the test substance. The actual solubility obtained was 48.89 mg/L. From this stock solution further test concentrations were prepared for achieving test concentrations of 0.8 mg/L, 1.76 mg/L, 3.87mg/L, 8.5mg/L, 18.72mg/L, respectively.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. The fishes were moving slowly as compared to control. No mortalities were observed in the control aquaria.
LC0 (96 hours) (highest loading at which no mortality was observed) = 8.51mg/L
LC50 (96 hours) Experimental = >8.51mg/L & <18.72mg/L
LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 18.72mg/L
LC50 (through probit analysis) = 10.06mg/L
After rounding-off the numerical values, the LC 50 was determine to be 10 mg/L.
Based on the LC50 value (10 mg/l), chemical 2,4,6-trichloroaniline, consider to be toxic and classified as aquatic chronic 2 as per the CLP classification criteria.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 10 mg/L
Additional information
Based on the experimental data from various database for the target chemical study have been reviewed to determine the mode of action of2,4,6-trichloroaniline (CAS no. 634-93-5)on the mortality rate and behavior of fish. The studies are as mentioned below:
In the first key study from experimental report 2015, study was conducted to access the effect of test chemical 2,4,6-trichloroaniline on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The nominal concentration selected for the experiment were 0.8 mg/L, 1.76 mg/L, 3.87mg/L, 8.5mg/L, 18.72mg/L, respectively and Zebra Fish Danio rerio were exposed to these concentration for 96 hours. The test substance was slightly soluble in water. Therefore, the stock solution was prepared by dissolving 1gm of the test substance in 1 liters of potable water (passed through reverse osmosis system) and kept it for 30min of sonication. After the completion of the sonication process, the sample was run through HPLC system for getting the actual water solubility of the test substance. The actual solubility obtained was 48.89 mg/L. From this stock solution further test concentrations were prepared for achieving test concentrations of 0.8 mg/L, 1.76 mg/L, 3.87mg/L, 8.5mg/L, 18.72mg/L, respectively. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. The fishes were moving slowly as compared to control. No mortalities were observed in the control aquaria.
LC0 (96 hours) (highest loading at which no mortality was observed) = 8.51mg/L
LC50 (96 hours) Experimental = >8.51mg/L & <18.72mg/L
LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 18.72mg/L
LC50 (through probit analysis) = 10.06mg/L
After rounding-off the numerical values, the LC 50 was determine to be 10 mg/L. Based on the LC50 value (10 mg/l), chemical 2,4,6-trichloroaniline, consider to be toxic and classified as aquatic chronic 2 as per the CLP classification criteria.
First key study was supported by the second study from authoritative database 2018. Study was conducted to determine the effect of test chemical on the mortality of fish for 96 hrs. Test performed according to the OECD Guideline 203 (Fish, Acute Toxicity Test). The LC50 (96 h) value of the test substance 2,4,6-trichloroaniline to fish is determine to be 5.3 mg/L. Thus considering the CLP Criteria for aquatic classification of the substance, it is concluded that 2,4,6-trichloroaniline exhibits short term toxicity to fish and is therefore classified as Aquatic Chronic category 2.
Similarly short term toxicity of test chemical was studied on the mortality rate of fish. Three gallon glass aquaria each holding 10 L of water were used. 10, 1, 0.1 mg/L concentrations prepared. 2 vessels for each concentration were used. The LC50 value of the test substance 2,4,6-trichloroaniline to Pimephales promelas is determine to be 1-10 mg/L on the basis of mortality. Thus considering the CLP Criteria for aquatic classification of the substance, it is concluded that 2,4,6-trichloroaniline exhibits short term toxicity to fish and is therefore classified as Aquatic Chronic category 2.
In the fourth supporting study from secondary sources 2018, short term toxicity of test chemical was studied on the mortality rate of fish. After the exposure of chemical with fish mortality were observed. On the basis of mortality observed, the LC50 value for the test substance 2,4,6-trichloroaniline to Leuciscus idus is observed to be 2.3 mg/L. Based on the LC50, chemical was consider as toxic and classified as aquatic chronic 2 as per the CLP classification criteria.
Based on the overall studies from experimental report 2015, authoritative databases and secondary sources 2018, it was concluded that the chemical 2,4,6-trichloroaniline was toxic and consider to be classified as aquatic chronic 2 as per the CLP classification criteria.
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