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EC number: 445-760-8 | CAS number: 122886-55-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 22 October 2002 - 24 October 2002
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- The test was conducted according to OECD Guideline 202 and in compliance with GLP. The stock solutions were prepared by agitation for 24 hours and then filtered twice (porosity of 4-7 µm and then of 0.45 µm). The filtered solution was then further used to prepare the test solutions. The measured concentrations of test substance in these solutions were below the limit of quantification (0.5 mg/L) at all measurement times. Based on the fact that the substance is highly insoluble in water (water solubility 4 µg/L) and has a high adsorptive potential (Koc of one constituent 4.8 and of the two other constituents> 5.6), it can only be concluded that the LC50 > maximum solubility of the test substance in medium
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 1000 mg/L
- Sampling method: samples taken at the begining of the test, at T24 and T48 h.
- Sample storage conditions before analysis: at -20 °C - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:stirring the test item in water for 24 h
- Evidence of undissolved material (e.g. precipitate, surface film, etc): the solution was filtered through a filter with 4-7 µm porosity, followed by filtration through 0.45 µm - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain: Straus-clone 5
- Source: Centra Technique du Bois et de l'Ameublement, Paris, France
- Age at study initiation (mean and range, SD): 6-24 h old
- Feeding during test: none
ACCLIMATION
- Acclimation period: from birth until use in the test
- Acclimation conditions (same as test or not): yes
- Type and amount of food: algal Scenedesmus subspicatus, 0.1-0.2 mg C/Daphnia/day
- Feeding frequency: daily
- Health during acclimation (any mortality observed):not reported - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- control t0 and t 48h: 289 and 272 mg/L
- Test temperature:
- control: 19.8 °C
limit test: 19.7 °C - pH:
- control t0 and t48h: 7.83 and 8.34
limit test: t0 and t 48h: 8.07 and 8.33 - Dissolved oxygen:
- control t0 and t48h: 9 and 8.2 mg/L
limit test: t0 and t 48h: 8.1 and 8.4 mg/L - Nominal and measured concentrations:
- Limit test nominal: 0 and 1000 mg/L
Limit test measured: < 0.5 mg/L (< LOQ)
Range finding test nominal: 1; 10 and 100 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: beaker
- Type: not reported
- Material, size, headspace, fill volume: not reported
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates):4
-Loading: one animal/10 mL culture water
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted M4
- Total organic carbon: not reported
- Particulate matter: not reported
- Metals: not reported
- Pesticides: not reported
- Chlorine: not reported
- Ca/mg ratio: 4:1
- Conductivity: not reported
- Culture medium different from test medium: no
- Intervals of water quality measurement: at the begining and end of the test
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light and 8 h dark
- Light intensity: not reported
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : EL50 (mobility)
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10 (range finding)
- Range finding study yes
- Test concentrations: 1, 10 and 100 mg/L (range finding)
-Limit test: 1000 mg/L - Reference substance (positive control):
- not specified
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: The LC50 was > maximum soluble concentration of test substance in medium.
- Details on results:
- - Behavioural abnormalities: no
- Observations on body length and weight: not reported
- Mortality of control: no
- Other adverse effects control: no
- Abnormal responses: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: not reported
- Effect concentrations exceeding solubility of substance in test medium: no
- analytics: measured concentrations < 0.5 mg/L (< LOQ)
The authors concluded that the 96h LC50 was > 1000 mg/L (initial loading rate). The French Authorities reported the 96h LC50 as > 4.38 µg/L (water solubility limit). - Validity criteria fulfilled:
- yes
- Conclusions:
- No effects were observed in the limit test, conducted with the filtrate of a 1000 mg/L loading rate. The measured concentrations of test substance in these solutions were below the limit of quantification (0.5 mg/L) at all measurement times. Based on the fact that the substance is highly insoluble in water (4 µg/L) and has a high adsorptive potential (Koc of one constituent 4.8 and of the two other constituents> 5.6), it can only be concluded that the EC50 > maximum solubility of the test substance in medium.
The Competent Authority of France concluded in 2003 that the EC50 for Daphnia magna should be >4.38 µg/L (the water solubility limit). - Executive summary:
The acute toxicity of KY-EU to Daphnia magna was examined in a static system according to OECD guideline 202 and GLP.
Daphnids were exposed to a WAF that was prepared at a loading rate of 1000 mg/L by stirring for 24h, followed by filtration through a filter with 4-7 µm porosity, and a filter with 0.45 µm porosity, subsequently. Samples were taken at the beginning of the test, at t = 24 and t = 48 h and stored at -20°C until analysis. Samples were analysed with HPLC-UV. The analysis was based on only one component out of 3 of KY-EU.
No effects of the test item to daphnia were observed.
The measured concentrations of test substance in these solutions were below the limit of quantification (0.5 mg/L) at all measurement times. Based on the fact that the substance is highly insoluble in water (4 µg/L) and has a high adsorptive potential (Koc of one constituent 4.8 and of the two other constituents> 5.6), it can only be concluded that the EC50 > maximum solubility of the test substance in medium.
Reference
Description of key information
No effects were observed in the limit test, conducted with the filtrate of a 1000 mg/L loading rate. The measured concentrations of test substance in these solutions were below the limit of quantification (0.5 mg/L) at all measurement times. Based on the fact that the substance is highly insoluble in water (4 µg/L) and has a high adsorptive potential (Koc of one constituent 4.8 and of the two other constituents> 5.6), it can only be concluded that the EC50 > maximum solubility of the test substance in medium.
Key value for chemical safety assessment
Additional information
Daphnids were exposed to a WAF that was prepared at a loading rate of 1000 mg/L by stirring for 24h, followed by filtration through a filter with 4-7 µm porosity, and a filter with 0.45 µm porosity, subsequently. Samples were taken at the begining of the test, at t = 24 and t = 48 h and stored at -20°C until analysis. Samples were analysed with HPLC-UV. The analysis was based on only one component out of 3 of KY-EU.
No effects of the test item to daphnia were observed.
The measured concentrations of test substance in these solutions were below the limit of quantification (0.5 mg/L) at all measurement times. Based on the fact that the substance is highly insoluble in water (4 µg/L) and has a high adsorptive potential (Koc of one constituent 4.8 and of the two other constituents> 5.6), it can only be concluded that the EC50 > maximum solubility of the test substance in medium.
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