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EC number: 203-185-8 | CAS number: 104-21-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2000-07-16 and 2000-07-17
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- 1997
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- p-methoxybenzyl acetate
- EC Number:
- 203-185-8
- EC Name:
- p-methoxybenzyl acetate
- Cas Number:
- 104-21-2
- Molecular formula:
- C10H12O3
- IUPAC Name:
- 4-methoxybenzyl acetate
Constituent 1
Method
- Target gene:
- Salmonella typhimurium: histidine
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Metabolic activation:
- with and without
- Metabolic activation system:
- Liver homogenate (S9) from Aroclor 1254 pretreated male rats
- Test concentrations with justification for top dose:
- 0, 15, 50, 150, 500, 1500, 5000 µg/plate
- Vehicle / solvent:
- - Vehicle/solvent used: DMSO
Controlsopen allclose all
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- 2-nitrofluorene
- sodium azide
- mitomycin C
- Remarks:
- without metabolic activation
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene (TA 98, TA 100, TA 102, TA 1535, TA 1537)
- Remarks:
- with metabolic activation
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Exposure duration: 48 to 72 h
NUMBER OF REPLICATIONS: two independent experiments with three replications
DETERMINATION OF CYTOTOXICITY
- Method: Number of revertant colonies - Evaluation criteria:
- The test substance is evaluated as mutagenic if it significantly or dose dependently increases the mutation frequency of the tester strains.
- Statistics:
- For estimation of the statistical significance of the difference between the mean number of revertants in the negative controls and the plates at each dosage level a X2-test was used.
Results and discussion
Test results
- Key result
- Species / strain:
- S. typhimurium, other: TA1537, TA1535, TA98, TA100 and TA102
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Bacteriotoxic towards the strains TA 1537 and TA 102 at 5000 µg/plate without S9 mix.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Precipitation: No precipitation was observed.
ADDITIONAL INFORMATION ON CYTOTOXICITY:
Bacteriotoxic towards the strains TA1537 and TA102 at 5000μg/plate in the absence of S9-mix.
Any other information on results incl. tables
Experiment 1:
Dose (µg/plate) |
Mean number of revertant colonies/3 replicate plates (± S.D.) with different strains of Salmonella typhimurium |
||||
|
TA98 |
TA100 |
TA102 |
TA1535 |
TA1537 |
|
|
||||
|
Results without S9 |
||||
Untreated |
30± 5 |
122 ± 9 |
279 ± 25 |
15 ± 1 |
8 ± 2 |
DMSO (50µL) |
25 ± 5 |
134 ± 5 |
246 ± 12 |
15 ± 3 |
9 ± 6 |
15 |
|
|
252 ± 13 |
|
|
50 |
24 ± 6 |
123 ± 6 |
243 ± 16 |
15 ± 4 |
7 ± 3 |
150 |
19 ± 2 |
123 ± 6 |
241 ± 16 |
15 ± 3 |
5 ± 3 |
500 |
30 ± 6 |
139 ± 1 |
215 ± 11 |
17 ± 3 |
9 ± 4 |
1500 |
15 ± 6 |
134 ± 12 |
197 ± 19 |
12 ± 4 |
8 ± 3 |
5000 |
21 ± 6 |
147 ± 5 |
108 ± 5 |
13 ± 4 |
4 ± 3 |
NaN3 (0.7) |
|
483 ± 15 |
|
428 ± 20 |
|
2-NF (2.5) |
368 ± 24 |
|
|
|
|
9-AA (50) |
|
|
|
|
187 ± 13 |
Mitomycin (0.15) |
|
|
727 ± 7 |
|
|
|
|
|
|
|
|
|
Results with S9 |
||||
Untreated |
26 ± 7 |
149 ± 6 |
267 ± 10 |
9 ± 1 |
17 ± 6 |
DMSO (50µL) |
21 ± 4 |
131 ± 8 |
147 ± 4 |
10 ± 2 |
16 ± 2 |
15 |
|
|
243 ± 8 |
|
10 ± 5 |
50 |
21 ± 6 |
133 ± 3 |
247 ± 12 |
12 ± 3 |
17 ± 4 |
150 |
35 ± 5 |
132 ± 9 |
251 ± 10 |
14 ± 2 |
11 ± 3 |
500 |
19 ± 3 |
131 ± 2 |
235 ± 10 |
13 ± 4 |
9 ± 5 |
1500 |
29 ± 4 |
133 ± 6 |
249 ± 14 |
10 ± 3 |
14 ± 4 |
5000 |
19 ± 2 |
140 ± 8 |
230 ± 2 |
9 ± 3 |
|
2-AA (0.7) |
1081 ± 29 |
|
|
|
|
2-AA (0.8) |
|
2394 ± 162 |
|
|
|
2-AA (1.0) |
|
|
|
523 ± 29 |
|
2-AA (1.5) |
|
|
1253 ± 45 |
|
210 ± 17 |
2-NF: 2-nitrofluorene; 9-AA: 9-aminoacridine; 2-AA: 2-aminoanthracene
Experiment 2:
Dose (µg/plate) |
Mean number of revertant colonies/3 replicate plates (± S.D.) with different strains of Salmonella typhimurium |
||||
|
TA98 |
TA100 |
TA102 |
TA1535 |
TA1537 |
|
|
||||
|
Results without S9 |
||||
Untreated |
16 ± 2 |
115 ± 11 |
249 ± 6 |
16 ± 3 |
8 ± 4 |
DMSO (50µL) |
14 ± 5 |
123 ± 4 |
237 ± 11 |
14 ± 3 |
9 ± 4 |
15 |
|
|
223 ± 9 |
|
|
50 |
14 ± 5 |
106 ± 9 |
240 ± 16 |
12 ± 4 |
9 ± 4 |
150 |
17 ± 2 |
116 ± 7 |
234 ± 9 |
12 ± 2 |
9 ± 4 |
500 |
13 ± 2 |
112 ± 11 |
198 ± 11 |
18 ± 4 |
6 ± 3 |
1500 |
14 ± 1 |
120 ± 10 |
195 ± 2 |
19 ± 4 |
9 ± 2 |
5000 |
10 ± 4 |
134 ± 4 |
111 ± 12 |
14 ± 3 |
5 ± 6 |
NaN3 (0.7) |
|
334 ± 24 |
|
180 ± 15 |
|
2-NF (2.5) |
339 ± 41 |
|
|
|
|
9-AA (50) |
|
|
|
|
331 ± 38 |
Mitomycin (0.15) |
|
|
1106 ± 112 |
|
|
|
|
|
|
|
|
|
Results with S9 |
||||
Untreated |
25 ± 6 |
129 ± 11 |
255 ± 6 |
12 ± 3 |
19 ± 5 |
DMSO (50µL) |
21 ± 2 |
120 ± 5 |
245 ± 8 |
10 ± 3 |
14 ± 6 |
50 |
20 ± 0 |
109 ± 1 |
223 ± 13 |
8 ± 2 |
13 ± 7 |
150 |
20 ± 3 |
109 ± 8 |
245 ± 7 |
13 ± 2 |
11 ± 2 |
500 |
24 ± 5 |
114 ± 3 |
230 ± 15 |
11 ± 5 |
11 ± 4 |
1500 |
21 ± 7 |
125 ± 9 |
204 ± 3 |
11 ± 3 |
13 ± 4 |
5000 |
21 ± 3 |
113 ± 13 |
193 ± 9 |
6 ± 2 |
14 ± 4 |
2-AA (0.7) |
1207 ± 57 |
1665 ± 51 |
|
|
|
2-AA (1.0) |
|
|
|
313 ± 8 |
|
2-AA (1.5) |
|
|
1265 ± 25 |
|
282 ± 23 |
2-NF: 2-nitrofluorene; 9-AA: 9-aminoacridine; 2-AA: 2-aminoanthracene
Applicant's summary and conclusion
- Conclusions:
- The test substance was not mutagenic to Salmonella typhimurium strains TA1535, TA1537, TA98, TA100, and TA102 in the presence and absence of a metabolizing system.
- Executive summary:
An in vitro reverse mutation assay study (Ames) was conducted according to OECD Guideline 471 in Salmonella typhimurium (TA98, TA100, TA102, TA1535 and TA1537). Experiments were performed as a plate incorporation assay. All tests were conducted in triplicate and concentrations between 15 μg/plate and 5000 μg/plate were tested. Strains were exposed to the test substance with and without metabolic activation with rat liver S9 mix. Cytotoxicity was observed without S9 in the strains TA 1537 and TA 102 at 5000 μg/plate. No substantial increase in revertant colony numbers of any of the tester strains was detected following treatment with the test substance at any dose level, neither in the presence nor absence of metabolic activation (S9 mix). There was also no tendency of higher mutation rates with increasing concentrations in the range below the generally acknowledged border of biological relevance. Therefore it was concluded that the test substance did not induce gene mutations and it was considered to be non-mutagenic.
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