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EC number: 273-761-1 | CAS number: 69012-64-2 Amorphous silicon dioxide particles from the volatilization and vaporization of furnace feed materials in the manufacture of ferrosilicon and silicon.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro cytogenicity / chromosome aberration study in mammalian cells
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 974
- Report date:
- 1974
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- no guideline available
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 473 (In Vitro Mammalian Chromosome Aberration Test)
- Deviations:
- yes
- Remarks:
- without external metabolic activation system
- Principles of method if other than guideline:
- Method: Culture technique, determination of cytotoxicity and test for chromosomal aberrations decribed and similar to current guideline (OECD 473)
- GLP compliance:
- no
- Type of assay:
- in vitro mammalian chromosome aberration test
Test material
- Reference substance name:
- 112926-00-8
- EC Number:
- 601-214-2
- Cas Number:
- 112926-00-8
- IUPAC Name:
- 112926-00-8
- Details on test material:
- Syloid 244 (named FDA 71-48): CAS-Name: Silica gel, cryst.-free; CAS-No. 112926-00-8
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- other: Human embryonic lung cells (WI-38)
- Metabolic activation:
- without
- Test concentrations with justification for top dose:
- 0.1, 1.0, and 10 µg/ml (note: mcg/ml is stated: Tab. Test I, p. 83)
Dose selection: 10 µg/ml was the highest level free of observable cell clumping (see Report p. 6/7). - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: water, 0.85 % saline
- Justification for choice of solvent/vehicle: suspension
Controls
- Negative solvent / vehicle controls:
- yes
- Remarks:
- saline
- Positive controls:
- yes
- Positive control substance:
- triethylenemelamine
- Remarks:
- Migrated to IUCLID6: 0.1 µg/ml
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in suspension, 5 x10^5 cells/mL
Mutations were quantified by counting anaphase aberrations.
DURATION
- Incubation temperature: 37 °C
- Exposure duration: 24 - 48 h
Fixation: absolute methanol:glacial acetic acid (3:1) for 30 min after centrifugation
STAIN (for cytogenetic assays): acetic acid-orcein stain (2.0 %)
NUMBER OF REPLICATIONS: 3/dose level
NUMBER OF CELLS EVALUATED: 100/dose level
DETERMINATION OF CYTOTOXICITY
- Method: mitotic index; other: "any cytopathic effect" at 24, 48, and 72 h after exposure to dose levels logarithmatically spaced
OTHER EXAMINATIONS:
- Determination of polyploidy: yes
Results and discussion
Test results
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Remarks:
- "clumping" of cells as criterion for maximum dose selection
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Remarks on result:
- other: other: Human embryonic lung cells (Wi-38)
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Anaphase aberrations (from Report Table p. 83)
Mitotic index1) |
No. of cells |
% cells with acentric frag. |
% cells with bridges |
% multipolar cells |
% cells other aberr.2) |
% cells with aberr. |
|
Syloid |
|||||||
0.1 µg/mL |
3 |
100 |
3 |
0 |
0 |
0 |
3 |
1.0 µg/mL |
4 |
100 |
4 |
1 |
0 |
0 |
5 |
10.0 µg/mL |
4 |
100 |
0 |
0 |
0 |
0 |
0 |
Saline |
3 |
100 |
1 |
0 |
0 |
0 |
1 |
TEM (0.1 µg/mL) |
2 |
100 |
13 |
4 |
1 |
3 (pp) |
21 |
1) % cells in mitosis: 200 cells observed/dose level
2) Cells with polyploidy, pulverisation (pp), or greater than 10 aberrations
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
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