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EC number: 272-911-3 | CAS number: 68920-03-6 A complex combination obtained by steam distillation of C16-18 and C18-unsatd. glycerides followed by condensation of the steam.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From March 10, 2010 to April 28, 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.6800 (Modified Activated Sludge, Respiration Inhibition Test for Sparingly Soluble Chemicals)
- Deviations:
- no
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Soybean oil, deodorizer distillate
- EC Number:
- 272-211-8
- EC Name:
- Soybean oil, deodorizer distillate
- IUPAC Name:
- 272-211-8
- Reference substance name:
- 68783-88-0
- Cas Number:
- 68783-88-0
- IUPAC Name:
- 68783-88-0
- Reference substance name:
- 'Soybean oil, deodorizer distillates'
- IUPAC Name:
- 'Soybean oil, deodorizer distillates'
- Reference substance name:
- Fatty acids and unsaponifiable matter of vegetable origin
- IUPAC Name:
- Fatty acids and unsaponifiable matter of vegetable origin
- Details on test material:
- - Name of test material (as cited in study report): 'Soybean oil, deodorizer distillates'
- Substance type: Distillates
- Physical state: Liquid, pasty / Brown
- Purity test date: October 27, 2009
- Expiration date of the lot/batch: April 27, 2010
- Storage condition of test material: In original container, at room temperature (15 - 25°C), in the dark
- Other:
- Solubility: Insoluble in water
Constituent 1
Constituent 2
Constituent 3
Constituent 4
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- Not applicable
Test solutions
- Vehicle:
- no
- Details on test solutions:
- TEST MATERIAL TREATMENT: To obtain a homogenous sample, the test material was heated at 60°C in a water bath for approximately 4 h. An aliquot
of the test material was filled into a bottle and was allowed to cool down to room temperature under agitation or stirring before the test was started.
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances):
the test material was directly dosed into test flask and tap water was added and the mixture was stirred for about 24 h at 21°C in the dark/diffuse light before adding the inoculum.
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Source: Municipal sewage treatment plant, Dermstadt, Germany. This STP predominantly receives domestic waste
- Preparation of inoculum for exposure: The sludge was washed by centrifugation and the supernatant liquid phase was decanted. The solid material was resuspended in tap water and again centrifuged. To this mixture, 50 mL synthetic sewage feed/L was added daily, starting 2 d prior to use, and the sludge was kept at room temperature under continuous aeration until use. The dry wt of the activated sludge was determined, and diluted to 3.8 g/L with tap water immediately before use. The pH of the activated sludge was 7.5.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- None
Test conditions
- Hardness:
- Not reported
- Test temperature:
- 21°C during dissolution phase (24 h)
19-21°C during pre-incubation (3 h)
17-19 °C during evaluation period - pH:
- At start of exposure (0 h): 7.4-7.5
At end of exposure (3 h): 8.3 - Dissolved oxygen:
- At start of exposure (0 h): 7.8-8 mg O2/L
At end of exposure (3 h): 8.1-8.5 - Salinity:
- Not reported
- Nominal and measured concentrations:
- Nominal concentration: 0, 10, 32, 100, 320, and 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass flasks and karlsruher flasks
- Type (delete if not applicable): Closed
- Material, size, headspace, fill volume: Glass flasks of 1 L volume and Karlsruher flasks of 250 mL volume
- Aeration: Compressed air (0.8L/min)
- No. of vessels per concentration (replicates): One
- No. of vessels per control (replicates): Two (Control received tap water, synthetic sewage and inoculum without addition of the test material)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Laboratory tap water
- Synthetic sewage composition:
16 g Peptone
11 g Meat extract
3 g Urea
0.7 g NaCI
0.4 g CaCl2.2H2O
0.2 g MgSO4.7H2O
2.8 g K2HPO4
filled up to 1 L with deionised water
OTHER TEST CONDITIONS: The pH and the oxygen concentrations were determined at the start and at the end of the incubation period in all treatments. The water temperature was measured in one control medium at the start and the end of the incubation period.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Well-mixed sample of each test medium was poured into Karlsruher flask after 3 h of incubation time for the measurement of the respiratory rate. The oxygen concentration was measured with an oxygen electrode and was recorded for about 10 min. The oxygen consumption was determined from the most linear part of the respiration curve.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.2
- Range finding study: Range finding study was not conducted. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol (lot# 93297LJ and 98.9% purity) was used at a concentration of 3.2, 10, and 32 mg/L.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: If a deviation of ≤15% between test material treated activated sludge and control is considered not be an effect
- Details on results:
- - The respiration rates of the activated sludge treated with the test material at conc. between 10 to 1000 mg/L differed by less than 10% from control. - Based on the measured inhibition rates, the 3 h EC10, EC20 and EC50 could not be quantified because up to the highest nominal test concentration of 1000 mg/L less than 10% inhibition was noted (after 3 h incubation time). No dose related response was found. The maximum deviation in respiration rates was 7.7% at the conc. of 100 and 320 mg/L test material. Thus the 3h EC10, EC20 and EC50 are higher than 1000 mg/L under the test conditions.
- If a deviation of ≤15% between test material treated activated sludge and control is considered not be an effect, the NOEC of test material will be established above 1000 mg/L (nominal). - Results with reference substance (positive control):
- - Results with reference substance valid: Yes. The 3 h EC50 was 6.5 mg/L and is thus in the range of 5-30 mg/L for the used activated sludge batch.
- Other: The respiration rate of the activated sludge was moderately inhibited by 33.3% at the lowest nominal conc. of 3.2 mg/L. At the nominal conc. of 10 and 32 mg test material/L, the respiration rate was inhibited by 61.5 and 84.6% respectively. - Reported statistics and error estimates:
- The 3 h EC50 including the 95% confidence limits of the reference material was calculated by probit analysis.
Any other information on results incl. tables
Control results: The respiration rates of the two controls did not differ by more than 15%. The coefficient of variation was 1.8%. Hence, the validity criterion was fulfilled.
Table 1: Influence of test material on oxygen consumption of activated sludge
Test concentration (mg/L) | Oxygen consumption (mg O2/L min) | Inhibition % | pH | Oxygen concentration (mg O2/L) | ||
Start* | End* | Start* | End* | |||
1000 | 0.4 | -2.6 | 7.4 | 8.3 | 7.8 | 8.1 |
320 | 0.36 | 7.7 | 7.5 | 8.3 | 7.8 | 8.5 |
100 | 0.36 | 7.7 | 7.5 | 8.3 | 8 | 8.4 |
32 | 0.38 | 2.6 | 7.5 | 8.3 | 8 | 8.4 |
10 | 0.38 | 2.6 | 7.5 | 8.3 | 8 | 8.5 |
Positive control | ||||||
32 | 0.06 | 84.6 | 7.5 | 8.3 | 8 | 9.1 |
10 | 0.15 | 61.5 | 7.5 | 8.3 | 8 | 9.0 |
3.2 | 0.26 | 33.3 | 7.5 | 8.3 | 7.9 | 8.7 |
*start and end of 3 h aeration time
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Less than 10% inhibition was noted up to the highest test conc. (1000 mg/L). Hence, under the test conditions, 3h EC50 of test material was found to >1000 mg/L (nominal) in the activated sludge respiration inhibition test.
- Executive summary:
A study was conducted to assess the effect of the test substance 'Soybean oil, deodorizer distillates' on the respiration of activated sewage sludge according to OECD Guideline No. 209, EU method C.11 and US EPA Guideline OPPTS 850.6800.
Activated sewage sludge was exposed to the test material at concentrations of 0, 10, 32, 100, 320, and 1000 mg/L for a period of 3 h at a temperature of 19-21°C with the addition of a synthetic sewage as a respiratory substrate. The rate of respiration was determined after 3 h contact time and compared to data for the control and a reference substance, 3,5-dichlorophenol.
Less than 10% inhibition was noted up to the highest nominal test conc. (1000 mg/L). No dose related response was found. The maximum deviation in respiration rates was 7.7% at the conc. of 100 and 320 mg/L test material. Thus the 3h EC10, EC20 and EC50 are higher than 1000 mg/L. The 3 h EC50 of reference substance was 6.5 mg/L and is thus in the range of 5-30 mg/L. The respiration rates of the two controls did not differ by more than 15%.(Met the validity criteria of the test)
Hence, under the test conditions, 3h EC50 of test material was found to >1000 mg/L (nominal) in the activated sludge respiration inhibition test.
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