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Diss Factsheets
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EC number: 203-561-1 | CAS number: 108-21-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Additional information
Isopropyl acetate was not mutagenic in a standard Ames assay (TA97, TA98, TA 100, TA 1535 and TA 1537) when tested to a maximum concentration of 10 mg/plate in the presence and absence of metabolic activation (Zeiger, et al., 1992). Zimmermann et al (1989) reported that isopropyl acetate was a weak inducer of chomosomal malsegregation in Saccharomyces cerevesiae, and potentiated this effect when administered in combination with a potent inducer, propionitrile. Similar results have been reported in this assay for many aprotic polar solvents. The relevance of these findings to human health are not understood.
The frequency of chromosome aberrations was evaluated in Chinese Hamster Ovary cells exposed to ethyl acetate (a substance very similar to isopropyl acetate) at concentrations up to 1500 ug/ml in a well reported study that was similar to guideline. Results were negative with and without metabolic activation up to the highest dose tested of 15mg/ml.
An in vivo mouse micronucleus test was conducted with the in vivo hydrolysis product, isopropanol, by intraperitoneal injection (Kapp, et al., 1993). Isopropyl alcohol was injected once to male and female ICR mice (17 per sex per dose) at doses up to 2,500 mg/kg bw body weight. The incidence of mortality was 0, 0, and 15 % among animals treated with isopropyl alcohol at doses of 350, 1173, and 2500 mg/kg bw. Bone marrow cells were collected from treated mice (5/sex/dose) at 24, 48, and 72 hours after treatment. Isopropyl alcohol did not produce an increase in the incidence of micronuclei at any dose level. There was no effect on the P/N ratio in among mice treated with isopropyl alcohol.
Isopropanol was not mutagenic in the Chinese Hamster Ovary HGPRT locus gene mutation assay in vitro (with and without energy-supplmented S-9 mix) at sub-cytotoxic concentrations up to 5.0 mg/ml.
The evidence from data generated on isopropyl acetate itself, close structural analogues such as ethyl acetate and on the rapidly produced in vivo metabolite isopropanol indicate that isopropyl acetate is very unlikely to exhibit genotoxic or mutagenic properties.
Short description of key information:
Isopropyl acetate is not mutagenic in the Ames assay. Like many aprotic polar solvents, isopropyl acetate has been reported to be a weak inducer of chromosomal malsegregation in Saccharomyces cerevesiae. The relevance of this finding to human health assessment is unclear.
The in vivo hydrolysis product, isopropanol, was negative in an in vivo mouse micronucleus study.
Endpoint Conclusion: No adverse effect observed (negative)
Justification for classification or non-classification
Based on the available experimental data, no classification is appropriate.
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