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EC number: 500-101-4 | CAS number: 38294-64-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: This study was conducted under GLPs and followed OECD guidelines. There were no deviations.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- not required
- Vehicle:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Activated sludge collected from the Cambridge Wastewater Treatment Facility, Cambridge, Maryland on 23 July 2012 was utilized as the inoculum for the test. The Cambridge facility receives wastes from predominately domestic sources. The sludge was sieved using a 2 mm screen and allowed to
settle for approximately 30 minutes. The supernatant above the settled solids was removed and the total suspended solids (TSS) concentration of the settled sludge was determined. Total suspended solids in the settled sludge were adjusted to a nominal concentration of approximately 4000 mg/L by dilution with municipal water. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Post exposure observation period:
- None
- Test temperature:
- 19.7 - 20.3 C
- pH:
- 8.0
- Nominal and measured concentrations:
- Nominal concentrations: 10, 100, 1000 mg/L
- Details on test conditions:
- Temperatures measured throughout the period of sludge maintenance and during the test ranged from 19.7 – 20.3°C. The initial total suspended solids (TSS) concentration of the activated sludge after removal of the supernatant layer measured 22,956 mg/L. The measured TSS concentration of the sludge on the day of testing was 4187 mg/L. The measured pH of the sludge on the day of testing was 8.0.
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- The respiration rates in the two controls were 93.6 and 102.9 mg O2/L/hr. Both controls had respiration rates above 33.5 mg O2/L/hr, which is the low limit based on the validity criteria for mixtures with 1.67 g dry weight of suspended solids per liter. The coefficient of variation of the two control respiration rates was 6.7%, and was within the 30% limit established for the test. The validity of the test was further supported by the results from the 3,5-dichlorophenol reference group, which resulted in an EC50 value of 22.4 mg/L, with 95 percent confidence limits of 15 mg/L and 50 mg/L. The EC50 for the reference substance was within the 2 to 25 mg/L range considered acceptable for the test.
- Results with reference substance (positive control):
- The 3,5-dichlorophenol reference group resulted in an EC50 value of 22.4 mg/L, with 95 percent confidence limits of 15 mg/L and 50 mg/L.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 value for BADGE IPD (#33) is greater than 1000 mg/L, the highest concentration tested.
- Executive summary:
The effect of BADGE IPD (#33) on activated sludge microorganisms maintained in an aerobic environment was assessed by the Activated Sludge Respiration Inhibition Test Method (OECD Guideline 209). The test contained control, reference and treatment groups. The control replicates were used to determine the background respiration rate of the sludge and were not dosed with the test or reference substance. The reference group was dosed with 3,5-dichlorophenol, a known inhibitor of respiration, at concentrations of 3, 15 and 50 mg/L. The treatment group was dosed with BADGE IPD (#33) at concentrations of 10, 100, and 1000 mg/L. The 1000 mg/L treatment was tested in triplicate. An abiotic control was dosed with the test substance at a concentration of 1000 mg/L to discriminate between abiotic uptake by the test substance and microbial respiration.
The respiration rates in the two controls were 93.6 and 102.9 mg O2/L/hr. Both controls had respiration rates above 33.5 mg O2/L/hr, which is the low limit based on the validity criteria for mixtures with 1.67 g dry weight of suspended solids per liter. The coefficient of variation of the two control respiration rates was 6.7%, and was within the 30% limit established for the test. The validity of the test
was further supported by the results from the 3,5-dichlorophenol reference group, which resulted in an EC50 value of 22.4 mg/L, with 95 percent confidence limits of 15 mg/L and 50 mg/L. The EC50 for the reference substance was within the 2 to 25 mg/L range considered acceptable for the test. An inhibitory dose response effect was observed for the treatment groups; however, the EC50 value
for BADGE IPD (#33) is greater than 1000 mg/L, the highest concentration tested. The abiotic treatment mixture dosed with 1000 mg/L of BADGE IPD (#33) had a respiration rate of –0.6 mg O2/L/hr showing there was no chemical uptake of oxygen by the test substance.
Reference
Description of key information
The effect of BADGE IPD (#33) on activated sludge microorganisms maintained in an aerobic environment was assessed by the Activated Sludge Respiration Inhibition Test Method (OECD Guideline 209).
Key value for chemical safety assessment
Additional information
The effect of BADGE IPD (#33) on activated sludge microorganisms maintained in an aerobic environment was assessed by the Activated Sludge Respiration Inhibition Test Method (OECD Guideline 209). The test contained control, reference and treatment groups. The control replicates were used to determine the background respiration rate of the sludge and were not dosed with the test or reference substance. The reference group was dosed with 3,5-dichlorophenol, a known inhibitor of respiration, at concentrations of 3, 15 and 50 mg/L. The treatment group was dosed with BADGE IPD (#33) at concentrations of 10, 100, and 1000 mg/L. The 1000 mg/L treatment was tested in triplicate. An abiotic control was dosed with the test substance at a concentration of 1000 mg/L to discriminate between abiotic uptake by the test substance and microbial respiration.
The respiration rates in the two controls were 93.6 and 102.9 mg O2/L/hr. Both controls had respiration rates above 33.5 mg O2/L/hr, which is the low limit based on the validity criteria for mixtures with 1.67 g dry weight of suspended solids per liter. The coefficient of variation of the two control respiration rates was 6.7%, and was within the 30% limit established for the test. The validity of the test
was further supported by the results from the 3,5-dichlorophenol reference group, which resulted in an EC50 value of 22.4 mg/L, with 95 percent confidence limits of 15 mg/L and 50 mg/L. The EC50 for the reference substance was within the 2 to 25 mg/L range considered acceptable for the test. An inhibitory dose response effect was observed for the treatment groups; however, the EC50 value for BADGE IPD (#33) is greater than 1000 mg/L, the highest concentration tested. The abiotic treatment mixture dosed with 1000 mg/L of BADGE IPD (#33) had a respiration rate of –0.6 mg O2/L/hr showing there was no chemical uptake of oxygen by the test substance.
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