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Diss Factsheets
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EC number: 204-445-3 | CAS number: 121-03-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 437 (Bovine Corneal Opacity and Permeability Test Method for Identifying Ocular Corrosives and Severe Irritants)
- Version / remarks:
- 2009
- Principles of method if other than guideline:
- Bovine Corneal Opacity and Permeability Test (BCOP-Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 4-nitrotoluene-2-sulphonic acid
- EC Number:
- 204-445-3
- EC Name:
- 4-nitrotoluene-2-sulphonic acid
- Cas Number:
- 121-03-9
- Molecular formula:
- C7H7NO5S
- IUPAC Name:
- 2-methyl-5-nitrobenzenesulfonic acid
Constituent 1
Test system
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 750 µl of a 20% test-substance preparation - Details on study design:
- Corneal opacity was measured quantitatively as the amount of light transmission through the cornea. Permeability was measured quantitatively as the amount of sodium fluorescein dye that passes across the full thickness of the cornea. Both measurements were used to calculate an In Vitro Irritancy Score (IVIS) of the test substance, which was used for prediction of serious eye damage.
Each treatment group (test substance, NC and PC) consisted of 3 corneas.
Before application the medium in the anterior chamber was removed using a syringe.
750 µL of the 20% (w/v) test-substance preparation (non-surfactant) was applied into the anterior chamber using a pipette.
Control tissues were concurrently applied into the anterior chamber with 750 µL of highly de-ionized water (negative control, NC) or with 750 µL of 20% (w/v) solution of Imidazole in highly de-ionized water (positive control, PC) using a pipette.
The corneas were incubated in a horizontal position at about 32 °C for approximately 4 hours (non-surfactant solids). The test substance, NC and PC were then removed from the anterior chamber using a syringe and the epithelium was washed at least 3 times with Eagle’s MEM (containing phenol red) and once with Eagle’s MEM (without phenol red). Both chambers were then refilled with fresh Eagle’s MEM (without phenol red).
Results and discussion
In vitro
Resultsopen allclose all
- Irritation parameter:
- in vitro irritation score
- Value:
- 143.6
- Vehicle controls validity:
- not examined
- Negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Irritation parameter:
- cornea opacity score
- Value:
- 143.4
- Vehicle controls validity:
- not examined
- Negative controls validity:
- not specified
- Positive controls validity:
- not specified
Any other information on results incl. tables
The BCOP test showed the following results: The mean opacity change of the test-substance treated corneas was 143.4. The mean OD490 of the test-substance treated corneas determined as permeability parameter was 0.013. The In Vitro Irritancy Score (IVIS) of the test-substance treated corneas was calculated to be 143.6.
Based on the observed results and applying the evaluation criteria it was concluded, that 4-Nitrotoluene-2-sulphonic acid causes serious eye damage in the Bovine Corneal Opacity and Permeability Test (BCOP Test) under the test conditions chosen.
Applicant's summary and conclusion
- Interpretation of results:
- other: classified in Category 1 according to the CLP Regulation EC No. 1272/2008
- Conclusions:
- The substance is an eye irritant.
- Executive summary:
The corneal damage potential of the substance was assessed according to OECD guideline 437.
Corneal opacity was measured quantitatively as the amount of light transmission through the cornea. Permeability was measured quantitatively as the amount of sodium fluorescein dye that passes across the full thickness of the cornea. The mean Opacity change of the test-substance treated corneas was 143.4. The mean OD490 of the test-substance treated corneas determined as permeability parameter was 0.013. The In Vitro Irritancy Score (IVIS) of the test-substance treated corneas was calculated to be 143.6.
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