Registration Dossier
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EC number: 231-609-1 | CAS number: 7651-02-7
Toxicity to soil microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- REPORTING FORMAT FOR THE ANALOGUE APPROACH
1. HYPOTHESIS FOR THE ANALOGUE APPROACH
This read-across is based on the hypothesis that source and target substances have similar toxicological properties because
• they are manufactured from similar or identical precursors under similar conditions
• they share structural similarities with common functional groups: quaternary amines, amides, and saturated fatty acid chains with comparable length
• the metabolism pathway leads to comparable products (amine backbone and long chain fatty acids) and non-common products predicted to have no toxicological effects (long chain fatty acids).
Therefore, read-across from the existing ecotoxicity (sediment and soil) and environmental fate studies on the source substance is considered as an appropriate adaptation to the standard information requirements of the REACH Regulation for the target substance, in accordance with the provisions of Annex XI, 1.5 of the REACH Regulation.
For further details, please refer to attached read-across justification.
2. SOURCE AND TARGET CHEMICAL(S) (INCLUDING INFORMATION ON PURITY AND IMPURITIES)
Please refer to attached read-across justification.
3. ANALOGUE APPROACH JUSTIFICATION
Please refer to attached read-across justification.
4. DATA MATRIX
Please refer to attached read-across justification. - Reason / purpose for cross-reference:
- read-across source
- Duration:
- 28 d
- Dose descriptor:
- EC50
- Effect conc.:
- 76 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- nitrate formation rate
- Remarks on result:
- other: (66.0 - 84.3)
- Duration:
- 28 d
- Dose descriptor:
- EC10
- Effect conc.:
- ca. 15 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- nitrate formation rate
- Remarks on result:
- other: ca. 12-38
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 10 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- nitrate formation rate
- Remarks on result:
- other: n.a.
- Conclusions:
- After 7 days of exposure the EC25-value for inhibition of the nitrate-N formation rate in soil was 22.0 mg/kg soil dry weight. After 7 days the inhibition of nitrate transformation was below 50 % at all test item concentrations resulting in an EC50-value > 500 mg/kg soil dry weight. The NOEC for nitrate transformation in soil on day 7 was determined to be 12.8 mg/kg soil dry weight.
After 14 days of exposure the EC25-value for inhibition of the nitrate-N formation rate in soil was < 32 mg/kg soil dry weight. The EC50 for inhibition of the nitrate transformation was determined to be at 176 mg/kg soil dry weight. The NOEC for nitrate transformation in soil on day 14 was set at 12.8 mg/kg soil dry weight.
After 28 days of exposure the following EC-values for inhibition of the nitrate-N formation rate were determined: EC12.5: 16.6 mg/kg soil dry weight, EC25: 49.5 mg/kg soil dry weight, EC50: 76.0 mg/kg soil dry weight. The NOEC for nitrate transformation in soil on day 28 was set < 12.8 mg/kg soil dry weight.
Reference
Description of key information
Key value for chemical safety assessment
- Long-term EC10 or NOEC for soil microorganisms:
- 12.8 mg/kg soil dw
Additional information
The toxicity to soil microorganisms od Quaternary ammonium compounds, C20-22-alkyltrimethyl, chlorides was tested according to OECD guideline 216 over a period of 28 d.
After 7 days of exposure the EC25-value for inhibition of the nitrate-N formation rate in soil was 22.0 mg/kg soil dry weight. After 7 days the inhibition of nitrate transformation was below 50 % at all test item concentrations resulting in an EC50-value > 500 mg/kg soil dry weight. The NOEC for nitrate transformation in soil on day 7 was determined to be 12.8 mg/kg soil dry weight.
After 14 days of exposure the EC25-value for inhibition of the nitrate-N formation rate in soil was 32 mg/kg soil dry weight. The EC50 for inhibition of the nitrate transformation was determined to be at 176 mg/kg soil dry weight. The NOEC for nitrate transformation in soil on day 14 was set at 12.8 mg/kg soil dry weight.
After 28 days of exposure the following EC-values for inhibition of the nitrate-N formation rate were determined: EC12.5: 16.6 mg/kg soil dry weight, EC25: 49.5 mg/kg soil dry weight, EC50: 76.0 mg/kg soil dry weight. The NOEC for nitrate transformation in soil on day 28 was set 12.8 mg/kg soil dry weight.
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