Methyl chloroformate

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: limited documentation and some deviations to OECD 405. The study is, however suffient for substance evaluation.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1975

Report date:

1975

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Deviations:

yes

Remarks:

only 50 µl applied

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Methyl chloroformate

EC Number:

201-187-3

EC Name:

Methyl chloroformate

Cas Number:

79-22-1

Molecular formula:

C2H3ClO2

IUPAC Name:

methyl chloroformate

Details on test material:

- Name of test material (as cited in study report): Chlorameisensäure methyl ester (chloroformic acid-methyl ester)
- Physical state: liquid
- no further data

Test animals / tissue source

Species:

rabbit

Strain:

Vienna White

Details on test animals or tissues and environmental conditions:

TEST ANIMALS
- Source: Gaukler, Germany
- Weight at study initiation: 2.79 and 2.80 kg

ENVIRONMENTAL CONDITIONS
no data

Test system

Vehicle:

unchanged (no vehicle)

Controls:

not required

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 50 µl

Duration of treatment / exposure:

eyes remained unwashed

Observation period (in vivo):

8 days

Number of animals or in vitro replicates:

2

Details on study design:

REMOVAL OF TEST SUBSTANCE
- Washing (if done): no washing done

SCORING SYSTEM: descriptive scoring systen comparable to the Draize score.
TOOL USED TO ASSESS SCORE: fluorescein to proof absebnce of any effect

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

cornea opacity score

Basis:

mean

Time point:

other: 27-48 h

Score:

2

Max. score:

4

Reversibility:

not reversible

Results 2

Irritation parameter:

iris score

Basis:

mean

Time point:

other: 24-48

Score:

0.5

Max. score:

2

Reversibility:

not reversible

Results 3

Irritation parameter:

chemosis score

Basis:

mean

Time point:

other: 24-48 h

Score:

1.5

Max. score:

4

Reversibility:

not reversible

Results 4

Irritation parameter:

conjunctivae score

Basis:

mean

Time point:

other: 24-48 h

Score:

1

Max. score:

4

Reversibility:

not reversible

Irritant / corrosive response data:

irreversible demage to the eyes

Any other information on results incl. tables

Descriptive scores of the raw data have been converted to Draize numerical scores.

Animal	Reading	Opacity	Iritis	Redness	Chemosis	Comment
1	1 h	2	0	1	0
2	1 h	2	0	1	0
1	24 h	2	0	1	3
2	24 h	1	0	1	0
1	48 h	2	2	1	3	suppuration
2	48 h	3	0	1	0	suppuration, corrossion
1	8 d	3	2	2	3	staphyloma/suppuration
2	8 d	3	0	2	3	staphyloma/suppuration
mean	24 - 48 h	2.0	0.5	1.0	1.5

 

Applicant's summary and conclusion

Conclusions:

risk of serious damage to eyes