Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 500-241-6 | CAS number: 69011-36-5 1 - 2.5 moles ethoxylated
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2019-07-03 to 2019-07-24
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Version / remarks:
- 2012
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Staatliches Gewerbeaufsichtsamt, Hildesheim, Germany
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: nominal loading rates 20.5, 51.2, 128, 320, 800 µg/L and the control (at least 3 times during the test in the fresh media at the start of an exposure interval (0 hours) and in the old media at the end of an exposure interval (48 or 72 hours)
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: water accommodated fraction (WAF) preparation according to OECD guidance document No. 23 (2018): One WAF of 1000 µg/L was prepared 24 ± 1 hour prior test start nd each renewal. Appropriate amount of the test item was applied by pipette into a glass flask filled with an appropriate amount of dilution water. Dispersion was shaken for 24 hours. After separation phase of at least 1 hour standing the WAF was removed by siphoning from the middle of the glass flask. Undissolved particles were removed by membrane filtration (membrane filter 0.45 µm, RC, Macherey-Nagel). The filter was saturated in order to avoid adsorption during the filtration. The first 25 mL of the filtrate were discarded. The filtration was interrupted for 15 minutes to allow adsorption and saturation of the filter material with dissolved test item. Thereafter, the filtration was continued. The next 25 mL were discarded. Five loadings of the test item were prepared from the Water Soluble Fraction ith the nominal loading of 1000 µg/L by dilution with dilution water to 20.5, 51.2, 128, 320, 800 µg/L.
- Differential loading: no
- Controls: dilution water only
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): No Tyndall effect was observed. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Age at study initiation: < 24 h
- Method of breeding: bred in g lass vessels (2 - 3 L capacity) with approximately 1.8 L culture medium, at 20 ± 2°C, in an incubator, 16 hours illumination; light intensity of max. 1500 lux, fed at least 5 times per week ad libitum with a mix of unicellular green algae
- Source: obtained from continuous laboratory cultures at Noack Laboratorien GmbH, Sarsted, Germany, orignally received from Institut für Wasser-, Boden- und Lufthygiene (WaBoLu), Berlin, Germany
- Feeding during test
- Food type: green algae, e.g. Pseudokirchneriella subcapitata and Desmodesmus subspicatus
- Amount: max. 0.2 mg C/daphnia and day
- Frequency: daily
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES, INCLUDING CULTURING CONDITIONS: juvenile daphnids were removed from the culture vessels at the latest 24 hours before the start of the exposure and discarded. The juveniles born within this period of max. 24 hours preceding the exposure were used for the test. No first brood progeny was used for the test. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Hardness:
- 214 - 288 mg CaCO3/L
- Test temperature:
- 20.5 - 21.8°C
- pH:
- 6.60 - 7.78
- Dissolved oxygen:
- 6.74 - 9.71
- Nominal and measured concentrations:
- Nominal loading rates: 0 (control), 20.5, 51.2, 128, 320, 800 µg/L
Time weighted mean measured: < LOQ (control), 20.9, 33.5, 84.8, 218, 542 µg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers (5 (ID) x 8 (H) cm), 100 mL capacity
- Type: loosely covered with watch glasses
- Fill volume: 50 mL
- Aeration: no
- Renewal rate of test solution: 3 times per week (i.e. on Monday, Wednesday and Friday)
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4, according to OECD 211, Annex 2
- Culture medium different from test medium: no
- Intervals of water quality measurement: once per week, at test start and at the end of exposure intervals (48 or 72 hours): pH-values, dissolved oxygen concentration, total hardness and water temperature
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16/8 hours light/dark cycle
- Light intensity: Max. 1500 lux
EFFECT PARAMETERS MEASURED: daily: mortality, number of neonates, number of aborted eggs or dead offspring, abnormalities; at test end: total length excluding the anal spine of each survived parental daphnid and the mean dry weight of the survived parental daphnids of all loading rates and the control
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.5
- Range finding study
- Test concentrations: 0, 100, 1000 µg/L
- Results used to determine the conditions for the definitive study: 0% mortality at 100 µg/L, 100% mortality at 1000µg/L - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate (24 h hours acute Immobilisation Test performed once per month)
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 218 µg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- EC10
- Effect conc.:
- 278 µg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: 95% confidence limits: 218 - 542 µg/L
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 218 µg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- - Other biological observations:
one male was identified at the loading rate 320 µg/L (replicate 8)
- Mortality of control: 0% - Results with reference substance (positive control):
- EC50: 1.78 mg/L (95% CI: 1.56 - 1.99 mg/L)
- Reported statistics and error estimates:
- Differences of reproduction were determined in comparison to the control using statistical standard procedures as normality test (Shapiro-Wilk’s test), variance homogeneity test (Levene’s test), Williams multiple sequential t-test for reproduction and step-down Cochran-Armitage test for adult mortality. Prior to running the multiple loading rate test, a normality test and a variance homogeneity test were performed. P-values of the normality and the variance homogeneity test were 0.01. The α-value (acceptable probability of incorrectly concluding that there is a difference) was α = 0.01 for the normality test and the variance homogeneity test.
- Validity criteria fulfilled:
- yes
- Remarks:
- For further details, refer to "Any other information on results incl. tables"
Reference
Table 1: Measured Concentrations of the Test Item during the Definitive Test (Days 0, 2, 9 and 12)
Sampling date |
Day 0 Fresh medium 0 hours |
Day 2 Old medium 48 hours |
Day 9 Fresh medium 0 hours |
Day 12 Old medium 72 hours |
||||
Nominal test item loading rate [µg/L] |
Test item |
|||||||
Meas. conc. [µg/L] |
% |
Meas. conc. [µg/L] |
% |
Meas. conc. [µg/L] |
% |
Meas. conc. [µg/L] |
% |
|
800 |
683 |
85 |
503 |
63 |
868 |
109 |
253 |
32 |
320 |
285 |
89 |
131 |
41 |
367 |
115 |
104 |
32 |
128 |
134 |
104 |
56.5 |
44 |
162 |
127 |
34.7 |
27 |
51.2 |
58.9 |
115 |
23.6 |
46 |
54.0 |
105 |
< LOQ |
- |
20.5 |
34.9 |
170 |
< LOQ |
- |
37.9 |
185 |
< LOQ |
- |
Control |
< LOQ |
< LOQ |
< LOQ |
< LOQ |
Meas.conc.= measured concentration of the testitem, dilution factors taken into account
% = percent of the nominal concentration of the test item
LOQ = limit of quantification (25.0µg/L)
Table 2: Effects on Reproduction for all Introduced Parents (based on the nominal loadings of the water accommodated fractions of the test item [µg/L])
Nominal loading rate of the test item [µg/L] |
Mean number of offspring per introduced parental daphnid |
||
Mean |
SD |
CV |
|
800 |
21.9 |
30.5 |
139 |
320 |
94.3 |
22.7 |
24.0 |
128 |
93.0 |
25.7 |
27.6 |
51.2 |
104 |
15.0 |
14.4 |
20.5 |
97.1 |
13.0 |
13.4 |
Control |
91.3 |
8.72 |
9.5 |
Table 3: Mortality [%] of the Adult Daphnids after 7, 14 and 21 Days of Exposure (n = 10)
Nominal loading rate of the test item [µg/L] |
Adult Mortality [%] |
||
7 days |
14 days |
21 days |
|
800 |
50 |
50 |
50 |
320 |
0 |
0 |
0 |
128 |
0 |
0 |
0 |
51.2 |
0 |
0 |
0 |
20.5 |
0 |
0 |
10 |
Control |
0 |
0 |
0 |
Table 4: Validity criteria for OECD 211.
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
The mortality of the parent animals in the control (female Daphnia) does not exceed 20% at the end of the test. |
0% |
yes |
The mean number of living offspring produced per parent animalsurvivingat the end of the test is ≥ 60 in the control. |
91.3 |
60 |
Description of key information
NOEC (21 d): 218 µg/L (TWA, OECD 211)
Key value for chemical safety assessment
Additional information
The chronic toxicity of Isotridecanol, ethoxylated, < 2.5 EO to aquatic invertebrates was studied in a tests following OECD 211. The semi-static test was set up with nominal loading rates of 20.5, 51.2, 128, 320, 800 µg/L (WAF). The test medium was renewed three times a week. An analytical dose verification by LC-MS/MS measurements in fresh (0 h) and aged media (48 or 72 h) was performed. The Time-weighted mean measured concentrations were 20.9, 33.5, 84.8, 218, 542 µg/L. The mortality, number of neonates, number of aborted eggs or dead offspring, abnormalities were recorded daily. Furthermore at test end, the total length excluding the anal spine of each survived parental Daphnids, and the mean dry weight of the survived parental Daphnids of all loading rates and the control were determined. Mortality of adult Daphnids was observed at the highest test concentration leading to a significantly reduced number of offspring at this concentration. Based on the cumulative offspring per introduced parent a NOEC (21 d) of 218 µg/L (TWA) was recorded.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.