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EC number: 939-290-7 | CAS number: 68607-20-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- July - September 1995
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- other: ISO 6341 (second edition; 1989)
- GLP compliance:
- not specified
- Analytical monitoring:
- no
- Details on sampling:
- 2 replicates of 10 animals/dose.
- Vehicle:
- yes
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Strain: Daphnia magna Straus
-Souce: Elf Atochom S.A.
- Age at study initiation: <24 hours
- Feeding during study: none
- Control: Potassium dichloride - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Test temperature:
- room temperature range from 19 to 21°C
- pH:
- 7.7
- Dissolved oxygen:
- 8.6
- Nominal and measured concentrations:
- Nominal concentration: 0; 0.006; 0.0095; 0.0153; 0.0244; 0.0391; 0.0625; 0.1 mg/L
- Details on test conditions:
- Test medium (25 mL of each solution):
- 11.76 g of Cacl2, 2H2O per liter of ultrapure water
- 4.93 g of MgSO4, 7H2O per liter of ultrapure water
- 2.59 g of NaHCO3 per liter of ultrapure water
- 0.23 g of KCl per liter of ultrapure water
Medium saturated in oxygen.
The nominal concentration of the eluate was 100 mg/L. By diluting this eluate with the medium the following nominal concentrations were prepared: 0; 0.006; 0.0095; 0.0153; 0.0244; 0.0391; 0.0625; 0.1 mg/L. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichlorate
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 0.094 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: equivalent to 0.080 mg a.i./L
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 0.059 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: equivalent to 0.050 mg a.i./L
- Details on results:
- OXYGEN CONTENT
At each concentration, the dissolved oxygen content, measured at the end of the test, was greater than OR equal to 2 mg/L
IMMOBILIZATION OF WITNESSES
Immobilization after 48 hours in the control series is 5%. The clause requiring a mortality lower OR equal to 10% at the end of the test has therefore been respected.
SENSITIVITY OF BIOLOGICAL REAGENT
The last control of the sensitivity of daphnia to potassium dichromate was carried out on 6/9/95. The EC (I) 50 - 24 h of this reference substance, evaluated at 0.94 mg/L, was between 0.9 mg/L and 2.4 mg/L which is the acceptable range (ISO 6341 standard). - Validity criteria fulfilled:
- yes
- Conclusions:
- Under the study conditions, the 48 h EC50 of the test substance to Daphnia was determined to be 0.059 mg/L (equivalent to 0.050 mg a.i./L).
- Executive summary:
A study was conducted to determine the short-term toxicity of the test substance, C16 -18 ADBAC (85% active in isopropanol) to aquatic invertebrates, according to ISO 6341 Method. Daphnia magna (2 replicates of 10 animals/dose) were exposed to the test substance at nominal concentrations of 0, 0.006, 0.0095, 0.0153, 0.0244, 0.0391, 0.0625 and 0.1 mg/L for 48 hours under static conditions at 20°C. Animals were observed for immobilisation after 24 and 48 h. Potassium dichlorate was used as the positive control. No analytical determination of the test substance was performed. At each concentration, the dissolved oxygen content, measured at the end of the test, was greater than or equal to 2 mg/L. Immobilization after 48 hours in the control series was 5%. The last control of the sensitivity of daphnia to potassium dichromate was carried out on 6/9/95. The EC50 (24 h) of the reference substance was determined to be 0.94 mg/L (between 0.9 mg/L and 2.4 mg/L which is the acceptable range of ISO 6341 standard). Under the study conditions, the 48 h EC50 of the test substance to Daphnia was determined to be 0.059 mg/L (equivalent to 0.050 mg a.i./L) nominal (Thiébaud, 1995).
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Study period:
- From May 09, 2003 to May 16, 2003
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods
- Justification for type of information:
- Refer to the Quaternary ammonium salts (QAS) category or section 13 of IUCLID for details on the category justification.
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: ISO/CD14669: "Determination of Acute Lethal Toxicity to Marine Copepods"
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: PARCOM ring test Protocol: "Acute Toxicity to the Marine Capepod Acartia tansa"
- Deviations:
- no
- GLP compliance:
- yes
- Remarks:
- GLP number: 41849
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Preparation and application of test solution:
- Method: The test substance was soluble in water, so the sample preparation method for water soluble test substances was followed.
The test substance was dissolved in filtered and aerated seawater to prepare a stock solution with concentration corresponding to the highest test concentration. The stock solution was mixed in a 1L Erlenmeyer flask on a magnetic stirrer (~100 rpm) for 2-4h after covering the top to minimise the volatilization. Required concentrations were prepared form the stock solution.
- Controls: 4
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): water - Test organisms (species):
- other: Acartia tonsa
- Details on test organisms:
- Test organism
- Common name: Calanoid copepod
- Source: Eggs of test organisms were obtained from University of Copenhagen, Laboratory of marine biology, Helsingør, Denmark
- Date of arrival of eggs: January 22, 2003
- Eggs were stored in refrigerator. Synchronious culture was used. The animals were cultivated in small batches.
- Age at study initiation: 14d - Test type:
- not specified
- Water media type:
- saltwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 20.0 - 21.1°C
- Dissolved oxygen:
- 7.2 mg/L
- Nominal and measured concentrations:
- - Based on the results from the Skeletonema costatum range finding study, the following test concentrations (nominal whole product) were used for the definitive test: 0.05; 0.2; 0.8; and 3.2 mg/L.
- Reference substance (3,5-dichlorophenol) concentrations (nominal): 1.0 mg/L. - Details on test conditions:
- Test system
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
Effect parameters measured: Mortality
Test concentrations:
- Range finding study: Dose levels selection was based on Skeletonema costatum range finding study
- Test concentrations: 0.05; 0.2; 0.8; and 3.2 mg/L. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 0.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: Calculated
- Remarks:
- equivalent to 0.16 mg a.i./L
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC10
- Effect conc.:
- ca. 0.37 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: Calculated
- Remarks:
- equivalent to 0.296 mg a.i./L
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- ca. 0.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: Calculated
- Remarks:
- equivalent to 0.32 mg a.i./L
- Key result
- Duration:
- 48 h
- Dose descriptor:
- other: LC90
- Effect conc.:
- ca. 0.43 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: Calculated
- Remarks:
- equivalent to 0.34 mg a.i./L
- Details on results:
- - Mortality of control: 0
- Mortality with test substance: Refer to Table 1 - Results with reference substance (positive control):
- - Reference substance study dates: May 14, 2003 to the May 16, 2003
- Results with reference substance valid? Yes
- Mortality: 55% after 48 h - Reported statistics and error estimates:
- The LC50-value and 95% confidence intervals were calculated based from a Logistic regression model using JMP Program, version 3, 1998.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the results of the read across study, the 48 h NOEC, LC10, LC50 and LC90 values were considered to be 0.2, 0.37, 0.40 and 0.43 mg/L (equivalent to 0.16, 0.296, 0.32 and 0.34 mg a.i./L), respectively.
- Executive summary:
A study was conducted to determine the short-term toxicity of the read across substance, C12-14 ADBAC (60 -100% active) to aquatic invertebrates, according to ISO/CD14669 and PARCOM ring test Protocol, in compliance with GLP. Acartia tonsa were exposed for 48 h at nominal concentrations of 0, 0.05, 0.2, 0.8 and 3.2 mg/L, equivalent to 0, 0.04, 0.16, 0.64 and 2.56 mg a.i./L considering 80% as mean active. Analytical dose verification was not performed. The test was performed on 14 d old organisms under static conditions. The percent lethality was recorded after 24 and 48 h and the LC50-value along with 95% confidence intervals were calculated based from a Logistic regression model using JMP Program. To ensure reproducibility, a single concentration 1 mg/L of reference substance 3,5-dichlorophenol was included in the study, which indicated 55% mortality after 48 h. All validity criteria were fulfilled. Under the study conditions, the 48 h NOEC, LC10, LC50 and LC90 values were considered to be 0.2, 0.37, 0.40 and 0.43 mg/L (equivalent to 0.16, 0.296, 0.32 and 0.34 mg a.i./L), respectively (Haug, 2003). Based on the results of the read across study, similar effect levels can be expected for the test substance.
Referenceopen allclose all
For result tables and graph, kindly refer to the attached background material section of the IUCLID.
Test substance:
Results of the control and test substance groups are given below under tables:
Table 1. Test substance concentrations and mortality after 24 and 48h
Test concentration (mg/L) |
Parallel |
Number of organisms per vessel |
Mortality (24 h) |
Mortality (48 h) |
Control |
1 |
5 |
0 |
0 |
|
2 |
5 |
0 |
0 |
|
3 |
5 |
0 |
0 |
|
4 |
5 |
0 |
0 |
0.05 |
1 |
5 |
0 |
0 |
|
2 |
5 |
0 |
0 |
|
3 |
5 |
0 |
0 |
|
4 |
5 |
0 |
0 |
0.2 |
1 |
5 |
0 |
0 |
|
2 |
5 |
0 |
0 |
|
3 |
5 |
0 |
0 |
|
4 |
5 |
0 |
0 |
0.8 |
1 |
5 |
5 |
5 |
|
2 |
5 |
5 |
5 |
|
3 |
5 |
5 |
5 |
|
4 |
5 |
5 |
5 |
3.2 |
1 |
5 |
5 |
5 |
|
2 |
5 |
5 |
5 |
|
3 |
5 |
5 |
5 |
|
4 |
5 |
5 |
5 |
Table 2. Calculation of LC10, LC50and LC90-values after 24 and 48h (The 95% confidence range could not be calculated)
24 and 48h |
|
Effect level |
mean (log) |
LC10 |
-0.43 |
LC50 |
-0.40 |
LC90 |
-0.37 |
Effect level |
mean (inverse log) |
LC10 |
0.37 |
LC50 |
0.40 |
LC90 |
0.43 |
Summary of the results:
The LC50-value for the test substance was found to be 0.40 mg/L after 24 and 48 h. The 95% confidence range could not be calculated.
The NOEC-, LC10- and LC90-values after 24 and 48h were 0.2, 0.37 and 0.43 mg/L, respectively.
Reference substance:
The lethality with 1.0 mg/L 3,5-dichlorophenol was 55% after 48 h.
Description of key information
Based on the available information, the freshwater 48 h EC50 value of the test substance for effects on mobility ofDaphnia magnawas determined to be 0.05 mg a.i./L (nominal). Further, based on the results of the read across study with C12-14 ADBAC, the marine water 48 h EC50 value of the test substance for acute toxicity in Acartia tonsais considered to be 0.32 mg a.i. /L (nominal). These respective EC50 values in fresh and marine water have been considered further for hazard/risk assessment.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 0.5 mg/L
Marine water invertebrates
Marine water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 0.32 mg/L
Additional information
Freshwater
A study was conducted to determine the short-term toxicity of the test substance, C16 -18 ADBAC (85% active in isopropanol) to aquatic invertebrates, according to ISO 6341 Method. Daphnia magna (2 replicates of 10 animals/dose) were exposed to the test substance at nominal concentrations of 0, 0.006, 0.0095, 0.0153, 0.0244, 0.0391, 0.0625 and 0.1 mg/L for 48 hours under static conditions at 20°C. Animals were observed for immobilisation after 24 and 48 h. Potassium dichlorate was used as the positive control. No analytical determination of the test substance was performed. At each concentration, the dissolved oxygen content, measured at the end of the test, was greater than or equal to 2 mg/L. Immobilization after 48 hours in the control series was 5%. The last control of the sensitivity of daphnia to potassium dichromate was carried out on 6/9/95. The EC50 (24 h) of the reference substance was determined to be 0.94 mg/L (between 0.9 mg/L and 2.4 mg/L which is the acceptable range of ISO 6341 standard). Under the study conditions, the 48 h EC50 of the test substance to Daphnia was determined to be 0.059 mg/L (equivalent to 0.050 mg a.i./L; nominal) (Thiébaud, 1995).
Marinewater
A study was conducted to determine the short-term toxicity of the read across substance, C12-14 ADBAC (60 -100% active) to aquatic invertebrates, according to ISO/CD14669 and PARCOM ring test Protocol, in compliance with GLP. Acartia tonsa were exposed for 48 h at nominal concentrations of 0, 0.05, 0.2, 0.8 and 3.2 mg/L, equivalent to 0, 0.04, 0.16, 0.64 and 2.56 mg a.i./L considering 80% as mean active. Analytical dose verification was not performed. The test was performed on 14 d old organisms under static conditions. The percent lethality was recorded after 24 and 48 h and the LC50-value along with 95% confidence intervals were calculated based from a Logistic regression model using JMP Program. To ensure reproducibility, a single concentration 1 mg/L of reference substance 3,5-dichlorophenol was included in the study, which indicated 55% mortality after 48 h. All validity criteria were fulfilled. Under the study conditions, the 48 h NOEC, LC10, LC50 and LC90 values were considered to be 0.2, 0.37, 0.40 and 0.43 mg/L (equivalent to 0.16, 0.296, 0.32 and 0.34 mg a.i./L), respectively (Haug, 2003). Based on the results of the read across study, similar effect levels can be expected for the test substance.
Based on the available information, the freshwater 48 h EC50 value of the test substance for effects on mobility of Daphnia magna was determined to be 0.05 mg a.i./L (nominal). Further, based on the results of the read across study with C12-14 ADBAC, the marine water 48 h EC50 value of the test substance for acute toxicity in Acartia tonsa is considered to be 0.32 mg a.i. /L (nominal).
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