[N,N,N',N',N'',N''-hexaethyl-29H,31H-phthalocyaninetrimethylaminato(2-)-N29,N30,N31,N32]copper

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions (incomplete documentation)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1976

Report date:

1976

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

yes

Remarks:

(application volume exceeded 20 mL/kg bw in the highest dose group, 2 dose groups, 7 day observation period)

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

[N,N,N',N',N'',N''-hexaethyl-29H,31H-phthalocyaninetrimethylaminato(2-)-N29,N30,N31,N32]copper

EC Number:

249-125-4

EC Name:

[N,N,N',N',N'',N''-hexaethyl-29H,31H-phthalocyaninetrimethylaminato(2-)-N29,N30,N31,N32]copper

Cas Number:

28654-73-1

Molecular formula:

C47 H49 Cu N11

IUPAC Name:

Copper,[N,N,N',N',N'',N''-hexaethyl-29H,31H-phthalocyanine-C,C,C-trimethanaminato(2-)-κN29,κN30,κN31,κN32]-

Details on test material:

Please refer to confidential details on test material

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Gassner
- Weight range at study initiation: 185 -250 g (males); 164 - 196 g (females)

ENVIRONMENTAL CONDITIONS: not reported

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

CMC (carboxymethyl cellulose)

Remarks:

aqueous suspension in 0.5% CMC

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 35% (10000 mg/kg bw), 15% (1000 mg/kg bw)

MAXIMUM DOSE VOLUME APPLIED: 28.6 mL/kg bw

Doses:

1000 mg/kg bw (6.7 mL/kg bw) and 10000 mg/kg bw (28.6 mL/kg bw)

No. of animals per sex per dose:

5 rats

Control animals:

no

Details on study design:

- Duration of observation period following administration: 7 days
- Frequency of observations after application: day 0 (after 2-3 hours) and once daily on working days
- Frequency of weighing: before application, on day 2 - 3 and at termination
- Necropsy of survivors performed: yes

Statistics:

not performed

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 10 000 mg/kg bw

Based on:

test mat.

Remarks on result:

other: 1/10 animal died on day 2 of observation

Mortality:

10000 mg/kg bw: 1 animal (female) died on day 2 of observation
1000 mg/kg bw: no mortality

Clinical signs:

other: 10000 mg/kg bw: On day 1, blue colouring of faeces and slight apathy were observed. Within 2 days, the symptoms were fully reversed. 1000 mg/kg bw: On day 1, blue colouring of faeces was observed. On day 2, faeces colour was normal.

Gross pathology:

10000 mg/kg bw: animal that died: substance residue was found in the thorax. No abnormalities were found in the other animals.
1000 mg/kg bw: no abnormalities were found.