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EC number: 229-352-5 | CAS number: 6485-40-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 06-09-1999 to 10-09-1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- other company data
- Title:
- Unnamed
- Year:
- 1 999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Guideline No. 405 (Acute Eye Irritation/Corrosion) and EEC Guideline Method B.5
- GLP compliance:
- yes
Test material
- Reference substance name:
- L-p-mentha-1(6),8-dien-2-one
- EC Number:
- 229-352-5
- EC Name:
- L-p-mentha-1(6),8-dien-2-one
- Cas Number:
- 6485-40-1
- Molecular formula:
- C10H14O
- IUPAC Name:
- (5R)-2-methyl-5-(prop-1-en-2-yl)cyclohex-2-en-1-one
- Test material form:
- other: Colourless to slight yellowish, liquid
- Details on test material:
- - Name of test material (as cited in study report): CARVONE L
- Physical state: Colourless to slight yellowish, liquid
- Analytical purity: 99,4%
- Lot/batch No.: 50458154
- Expiration date of the lot/batch: At least 1 year from receipt (26.08.1999)
- Storage condition of test material: Cool and dry
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- other: New Zealand White (SPF albino)
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Source: Charles River, Deutschland GmbH, Stolzenseeweg 32-36, 88353 Kißleg, Germany
- Weight at study initiation: 1.7 - 2.0 kg
- Housing: Caged individually in PPO cages (floor area: 2576 cm2) with perforated floor.
- Diet (e.g. ad libitum): Pelleted complete rabbit diet "Altromin 2123" from Altromin, D-32791 Lage, Lippe, was available ad libitum
- Water (e.g. ad libitum): Domestic quality drinking water (acidified with hydrochloric acid to pH 2.5 in order to prevent microbial growth) ad libitum
- Acclimation period: 7 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20°C ± 3°C
- Humidity (%): 55% ± 15%
- Air changes (per hr): 10 times/hour
- Photoperiod (hrs dark / hrs light): 12 hrs dark/12 hrs light
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- not required
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.1 mL - Duration of treatment / exposure:
- 24 hrs
- Observation period (in vivo):
- 1 hr, 24 hr, 48 hr and 72 hr.
- Number of animals or in vitro replicates:
- 4 females
- Details on study design:
- REMOVAL OF TEST SUBSTANCE
- Washing (if done): No
SCORING SYSTEM: Method of Draize/Grading of Ocular Lesions in OECD Guideline 405 with additions noted below in Tables 1 and 2.
TOOL USED TO ASSESS SCORE: The eyes were examined and the grade of ocular reaction was recorded 1 hr and 24 hrs later. After the first 24 hr reading, fluorescein was instilled. After rinsing with 20 ml 0.9% sodium chloride solution the eyes were examincd again using UV-light to detect possible
corneal damage.
Only the left eye was treated. The right eye remained untreated and served as control.
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- cornea opacity score
- Remarks:
- - opacity
- Basis:
- mean
- Remarks:
- all animals
- Time point:
- 24/48/72 h
- Score:
- 0
- Irritation parameter:
- iris score
- Basis:
- mean
- Remarks:
- all animals
- Time point:
- 24/48/72 h
- Score:
- 0
- Irritation parameter:
- conjunctivae score
- Remarks:
- - redness
- Basis:
- mean
- Remarks:
- all animals
- Time point:
- 24/48/72 h
- Score:
- 0.4
- Irritation parameter:
- chemosis score
- Basis:
- mean
- Remarks:
- all animals
- Time point:
- 24/48/72 h
- Score:
- 0
- Irritant / corrosive response data:
- Individual animal data is presented below (Table 3)
Any other information on results incl. tables
Table 3: Scores for ocular lesions (all animals)
Rabbit No./ Weight per kg | 1h | 24h | 24h | 48h | 72h | Individual mean score* | |
F | |||||||
1627 / 2.0 | Cornea opacity, degree | 0 | 0 | 0 | 0 | 0 | 0 |
Cornea opacity, area | 0 | 0 | 0 | 0 | 0 | ||
Iris | 1 | 0 | - | 0 | 0 | 0 | |
Conjunctiva: Redness | 2 | 1 | - | 0 | 0 | 0.33 | |
Chemosis | 1 | 0 | - | 0 | 0 | 0 | |
Discharge | 0 | 0 | - | 0 | 0 | ||
1628 / 1.9 | Cornea opacity, degree | 0 | 0 | 0 | 0 | 0 | 0 |
Cornea opacity, area | 0 | 0 | 0 | 0 | 0 | ||
Iris | 0 | 0 | - | 0 | 0 | 0 | |
Conjunctiva: Redness | 2 | 1 | - | 0 | 0 | 0.33 | |
Chemosis | 1 | 0 | - | 0 | 0 | 0 | |
Discharge | 1 | 0 | - | 0 | 0 | ||
1629 / 1.7 | Cornea opacity, degree | 0 | 0 | 0 | 0 | 0 | 0 |
Cornea opacity, area | 0 | 0 | 0 | 0 | 0 | ||
Iris | 0 | 0 | - | 0 | 0 | 0 | |
Conjunctiva: Redness | 2 | 1 | - | 0 | 0 | 0.33 | |
Chemosis | 1 | 0 | - | 0 | 0 | 0 | |
Discharge | 1 | 0 | - | 0 | 0 | ||
1630/ 1.8 | Cornea opacity, degree | 0 | 0 | 0 | 0 | 0 | 0 |
Cornea opacity, area | 0 | 0 | 0 | 0 | 0 | ||
Iris | 1 | 0 | - | 0 | 0 | 0 | |
Conjunctiva: Redness | 2 | 1 | - | 1 | 0 | 0.67 | |
Chemosis | 1 | 0 | - | 0 | 0 | 0 | |
Discharge | 0 | 0 | - | 0 | 0 |
F: Reading after instillation of Fluorescein
* Only the scores from the readings after 24, 48 and 72 hours are included in the calculation of the individual mean scores
Table 4: Summary of mean values from Table 3
Mean Value | |
cornea opacity | 0 |
iris lesion | 0 |
redness of conjunctiva | 0.4 |
oedema of conjunctiva (chemosis) | 0 |
Applicant's summary and conclusion
- Interpretation of results:
- not irritating
- Conclusions:
- The substance L-carvone was not irritating in the eyes of New Zealand White rabbits.
- Executive summary:
In a primary eye irritation study (01628) 0.1mL of L-carvone (undiluted) was instilled into the conjunctival sac of the left eye of young adult New Zealand White (SPF albino) rabbits (4/females) for 24 hours. The eye was not washed after instillation of the test article. Animals then were observed for 1 hr, 24 hr, 48 hr and 72 hrs after dosing. Irritation was scored by the method of Draize/Table: Grading of Ocular lesions (OECD 405) with additions noted in Tables 1 and 2.
The following mean values, based on the results from the 24, 48 and 72 hour readings,were calculated - cornea opacity: 0; iris lesion: 0; oedema of conjunctiva (chemosis): 0; redness of conjunctiva: 0.4.
In this study, L-carvone is not an eye irritant based on the results of this OECD guideline/GLP study.
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