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EC number: 247-368-0 | CAS number: 25956-17-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental test result performed according to the guideline.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 100mg/L,respectively.
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Anytime Pet,Jaripatka, Nagpur
- Length at study initiation (length definition, mean, range and SD): average 1.8 cm
- Weight at study initiation (mean and range, SD): average 0.082 g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 16 hours light and 8 hours dark
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 155.5 mg of CaCO3
- Test temperature:
- 24.1 °C
- pH:
- 7.1
- Dissolved oxygen:
- 7.0 mg/L
- Nominal and measured concentrations:
- 100 mg/l
- Details on test conditions:
- TEST SYSTEM:
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes
OTHER TEST CONDITIONS:
- Photoperiod:16 hours light and 8 hours dark
- Withdrawal of food: 1 day before and during exposure - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: No mortality were observed
- Sublethal observations / clinical signs:
Mortality and symptoms:
Nominal conc. (mg/l)
Number of fish
Mortality (cumulated) after
24h
48h
72h
96h
0 (control)
8
0
0
0
0
100
8
0
0
0
0
Visible Symptoms:
Nominal conc. (mg/l)
Number of fish
Symptoms after
24h
48h
72h
96h
0 (control)
8
NS8
NS8
NS8
NS8
100
8
NS8
NS8
NS8
NS8
Explanation of symptoms:
A: apathy
N: Narcotic-like state
C: Swimming near water surface
M: Slow movements compared to control
NS: No abnormal symptoms
S: Surfacing behaviour
L: Loss of equilibrium
SM: Shimming movement
H1: Hyperactivity
H2: Hypo activity
B: Bending behavior
F: Fin rot
IS: Invert Swimming
SB: Swimming behaviour
RF: Respiratory Function
P: Pigmentation
pH and oxygen content:
Nominal conc. (mg/l)
pH readings after
24h
48h
72h
96h
0 (control)
6.8
7.4
6.7
7.3
100
6.4
6.3
6.1
6.8
Nominal conc. (mg/l)
Oxygen content (mg/l) after
24h
48h
72h
96h
0 (control)
4.6
6.9
6.7
6.2
100
6.5
5.03
4.4
6.7
Test temperature
Nominal conc. (mg/l)
Temperature (°C) after
24h
48h
72h
96h
0 (control)
35
36
35
35
100
35
35
34
34
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on nominal concentration, experimental median lethal Concentrations [LC-50 (96 h)] for test material on Zebra Fish (Danio rerio) was determine to be >100 mg/l.
- Executive summary:
Study was conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test) to assess the effect of test chemical on the mortality of fish Danio rerio. The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 100mg/L, respectively and Zebra Fish Danio rerio were exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to 100 mg/l nominal test concentrations, LC50 was determine to be > 100 mg/l. Thus, based on the LC50, it was considered that the chemical was not toxic and cannot be classified as per the CLP classification criteria.
Reference
Description of key information
Based on nominal concentration, experimental median lethal Concentrations [LC-50 (96 h)] for test material on Zebra Fish (Danio rerio) was determine to be >100 mg/l.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
Based on the experimental data for the target as well as read-across analogues which are extracted by using mechanistic approach and functionally and structurally similar to the target chemical, toxicity of test chemical were determined. The studies are summarized as below:
Study was conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test) to assess the effect of test chemical on the mortality of fish Danio rerio. The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 100mg/L, respectively and Zebra Fish Danio rerio were exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to 100 mg/l nominal test concentrations, LC50 was determine to be > 100 mg/l. Thus, based on the LC50, it was considered that the chemical was not toxic and cannot be classified as per the CLP classification criteria.
In a supportive evidence, a 96 h study was conducted to determine the effect of test chemical on fish Danio rerio, at a test concentrations of 6.25mg/L, 12.5mg/L, 25mg/L, 50mg/L, 100mg/L. Test was performed according to OECD Guideline 203 (Fish, Acute Toxicity Test). Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. Zebra Fish (Danio rerio) were exposed to this concentration for 96 hours. After 96 hours of exposure of test chemical at of 100mg/L, no mortality was observed. Therefore the LC50 was determined to be >100mg/L. Based on the LC50, the test chemical can be considered as non-toxic and can be concluded as not classified as per the CLP classification criteria.
Further to support first and second study again, A Fish Acute Toxicity test was performed to evaluate the toxic nature of test substance. Test was performed according to OECD Guideline 203. Potable water (passed through reverse osmosis system) was used for the preparation of test solution The test was performed on the limit concentration i.e nominal concentration selected for the experiment were 100 mg/L and test fish were exposed to these concentration for 96 hours.
Effect on the symptoms and the normal activity was checked in the interval of 24rs. pH, tempterature and dissolved oxygen was also checked. After 96hrs of exposure, the lethal concentration LC50 was determined to be >100 mg/L
Thus based on the LC50 it can be concluded that the chemical was nontoxic and was considered to be not classified as per the CLP classification criteria.
Hence from the above data, the test chemical was considered as nontoxic and was not classified as per the CLP classification criteria.
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