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EC number: 701-004-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
- Report date:
- 2016
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Hydrocarbons, C4-20, arom.-rich, alkene-manufg. byproduct
- EC Number:
- 310-089-0
- EC Name:
- Hydrocarbons, C4-20, arom.-rich, alkene-manufg. byproduct
- Cas Number:
- 102242-57-9
- IUPAC Name:
- 102242-57-9
- Details on test material:
- - Name of test material (as cited in study report): Hydrocarbons, C4-20, arom.-rich, alkene-manufg. byproduct
- Substance type: organic
- Physical state: liquid
- Lot/batch No.: P0160-2015
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: P0160-2015
- Expiration date of the lot/batch: 22 Jan 2017
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: stored in a freezer ( <-18°C).
- Stability under test conditions: stable
Sampling and analysis
- Analytical monitoring:
- no
- Remarks:
- Analyses of the test substance preparations were not conducted, since a reliable method for analyses in the required concentration range could not be developed.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
-The test substance is a mixture of sparingly water soluble components. Therefore test solutions were prepared separately for each test concentration as a water accommodated fraction (WAF) using a liquid-liquid saturator technique. For each test solution, a glass aspirator bottle (2-L) with a bottom side-outlet attached to a stopcock was filled with 2-L test media. Prior to use the test substance was homogenized by shaking the container. Then test substance (considering density = 0.8018 g/cm³) was pipetted carefully on the water surface. The bottle was closed and the solution was stirred on a magnetic plate for about 2 days. The stirring was slow (approx. 100 rpm) so that no vortex forms. The undissolved test substance remained on the surface and the required volume of the WAF (1.4-L per test solution) was removed from the bottom port. The WAFs were free of undissolved test substance, confirmed by using a laser light and observing the Tyndall effect.
All stock test solutions appeared colorless and clear.
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Selenastrum capricornutum and currently renamed as Raphidocelis subcapitata KORSHIKOV
- Source (laboratory, culture collection): Collection of algal cultures in University of Göttingen /Germany
- Method of cultivation: A stock algal culture is maintained continuously at the test facility. Before the exposure an inoculum culture is prepared from the stock culture and incubated for 4 days at 21 – 24 °C (max. temperature difference 2 °C). After this time, the inoculum culture is in exponential growth phase and can be used to initiate the test (study day 0).
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 23.3 – 23.5 °C
- pH:
- 7.9 - 9.8
- Nominal and measured concentrations:
- loading rate: 0 (control), 6.25, 12.5, 25, 50, 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flasks (nominal volume 250 mL) closed with glass plugs
- Type: closed
- Material, size, headspace, fill volume: approx. 300 mL (Erlenmeyer flasks were completely filled)
- Initial cells density: 0.3 x 104 cells/mL
- Control end cells density:
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
1 additional uninoculated (without algae) replicate per test group for background fluorescence correction.
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: (OECD medium) was prepared according to OECD Guideline
OTHER TEST CONDITIONS
- Sterile test conditions: An inoculum culture in exponential growth phase was prepared with an aliquot of the stock algal culture added to sterile test media to provide an initial cell density of 0.3 x 104 cells/mL.
- Light intensity and quality: Light intensity was adjusted to the lower end of the acceptable range, to control pH changes due to excessive algal growth in the closed test vessels
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Algal growth measured as in vivo chlorophyll-a fluorescence (pulsed excitation with light flashes having a wavelength of 430 nm)
TEST CONCENTRATIONS
- Test concentrations: The test concentrations were selected on the basis of a range finding test.
- Results used to determine the conditions for the definitive study: EfluorescenceC50 = between 10 and 100 mg/L (40 mg/L estimated); ErC50 = between 10 and 100 mg/L (33 mg/L estimated); - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- 21.6 mg/L
- Conc. based on:
- other: based on loading rate
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EL10
- Effect conc.:
- 14.7 mg/L
- Conc. based on:
- other: based on loading rate
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 25 mg/L
- Conc. based on:
- other: based on loading rate
- Basis for effect:
- growth rate
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
EC50 values of the reference substance potassium dichromate should be in the range: ErC50 = 0.92 – 1.46 mg/L after 72 hours for Pseudokirchneriella subcapitata.
- The ErC50 (72 h) of potassium dichromate was 0.951 mg/L (Date of the last control experiment: 18 Jan 2016 , project number:60E0063/04E013)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In a 72-hour algal growth study, cultures of Pseudokirchneriella subcapitata were exposed to Hydrocarbons, C4-20, arom.-rich, alkene-manufg. byproduct at loading rate of 0 (control), 6.25, 12.5, 25, 50, 100 mg/L under static conditions for in accordance with the OECD 201 guideline. The water pH, temperature and lighting intensity were all maintained within acceptable guideline specifications.
The 72 hour ErC50 (ErL50) value determined in this alga toxicity study was 21.6 mg/L based on the loading rate.
The concentration of test substance in test media could not be verified analytically, therefore the effect concentrations are based on the loading rate used to prepare the test substance WAF solutions. According to guidance in OECD 23, for tests with chemicals that cannot be quantified by analytical methods at the concentrations causing effects, the effect concentration can be expressed based on the nominal concentrations or in the case of water accommodated fractions on the loading rate.acceptable guideline specifications.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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