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EC number: 204-909-5 | CAS number: 128-80-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Data is from experimental lab report
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- other: European Economic Community (EEC), EEC directive 92/69, Part C: Methods for the determination of ecotoxicity, Publication No. L383, December 1992, C.2. "Acute Toxicity for Daphnia".
- Principles of method if other than guideline:
- The purpose of the toxicity test was to evaluate the influence of test material on the mobility of Daphnia magna.
- GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Details on sampling:
- No data available
- Vehicle:
- not specified
- Details on test solutions:
- test material was added to test medium at a nominal concentration of 100 mg/I. The mixture was treated with ultrasonic waves for 5 minutes.
Afterwards magnetic stirring was applied for a total period of three days in order to achieve maximum saturation of the test medium with the test substance. After stirring the 100 mg/I solution appeared to be a black turbid dispersion with a floating layer. One part of this 100 mg/I solution was filtered (Schleicher & Schuell 604 filter) in order to remove the larger (i.e. > circa 5 //m) undissolved test substance particles. The filtering resulted in a clear greyish coloured solution. The other part of the 100 mg/I solution was not filtered and used as such. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Age of parental stock : < 24 hours
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- observations were made at 24 and 48 h
- Hardness:
- ISO-medium, hardness: 218 mg CaC03/l
- Test temperature:
- Between 20.1 °C and 20.8°C
- pH:
- described in table no.
- Dissolved oxygen:
- described in table no.
- Nominal and measured concentrations:
- 1.filtered solution prepared at a nominal concentration of 100 mg/I.
2.unfiltered solution prepared at a nominal concentration of 100 mg/l.
3.Blank-control medium - Details on test conditions:
- Loading: 10 Daphnia per vessel containing 80 ml test solution
Number of replicates: 2 replicates for each concentration - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Under the conditions of the present study test material did not induce acute immobilisation of Daphnia magna at a nominal concentration of 100 mg/l after 48 hours of exposure (NOEC).
- Executive summary:
Test material was used as a test material to evaluate its influence on the mobility of Daphnia magna. test material was added to test medium at a nominal concentration of 100 mg/I. The mixture was treated with ultrasonic waves for 5 minutes. Afterwards magnetic stirring was applied for a total period of three days in order to achieve maximum saturation of the test medium with the test substance. After stirring the 100 mg/I solution appeared to be a black turbid dispersion with a floating layer. One part of this 100 mg/I solution was filtered in order to remove the larger undissolved test substance particles. The filtering resulted in a clear greyish coloured solution. The other part of the 100 mg/I solution was not filtered and used as such. Under the conditions of the present study test material did not induce acute immobilisation of Daphnia magna at a nominal concentration of 100 mg/l after 48 hours of exposure (NOEC).Based on the NOEC value it can be concluded that test material has no hazardous effect on aquatic invertebrate and can not be classified as per CLP criteria.
Reference
MACROLEX GREEN5B (mg/I) |
Vessel number |
Number of Daphnia exposed |
Response after 24h |
Response after 48 h
|
|||
Number
|
% |
Number |
% |
||||
Blank- control |
A B |
83) 10 |
0(1) 0 |
0 0 |
0 0 |
0 0 |
|
1001) |
A#) B#) |
10 10 |
0*) 0*) |
0 0 |
0*) 0*) |
0 0 |
|
1002) |
A B |
10 10 |
01) 01) |
0 0 |
01) 01) |
0 0 |
#):precipitate and floating layer observed prior to the start of the test.
‘) :precipitate and floating layer observed
I ):precipitate observed.
1 ):unfiltered solution.
2) : filtered solution.
3) : inadvertently 8 instead of 10 Daphnia were exposed in replicate A of the blank-control
Description of key information
Short-term toxicity to aquatic invertebrates:
Test material was used as a test material to evaluate its influence on the mobility of Daphnia magna. test material was added to test medium at a nominal concentration of 100 mg/I. The mixture was treated with ultrasonic waves for 5 minutes. Afterwards magnetic stirring was applied for a total period of three days in order to achieve maximum saturation of the test medium with the test substance. After stirring the 100 mg/I solution appeared to be a black turbid dispersion with a floating layer. One part of this 100 mg/I solution was filtered in order to remove the larger undissolved test substance particles. The filtering resulted in a clear greyish coloured solution. The other part of the 100 mg/I solution was not filtered and used as such. Under the conditions of the present study test material did not induce acute immobilisation of Daphnia magna at a nominal concentration of 100 mg/l after 48 hours of exposure (NOEC).Based on the NOEC value it can be concluded that test material has no hazardous effect on aquatic invertebrate and can not be classified as per CLP criteria.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
Short-term toxicity to aquatic invertebrates:
To evaluate toxicity of aquatic invertebrates, various experimental studies were performed.Based on the experimental data aquired from various sources following is the discription:
Test material was used as a test material to evaluate its influence on the mobility of Daphnia magna. test material was added to test medium at a nominal concentration of 100 mg/I. The mixture was treated with ultrasonic waves for 5 minutes. Afterwards magnetic stirring was applied for a total period of three days in order to achieve maximum saturation of the test medium with the test substance. After stirring the 100 mg/I solution appeared to be a black turbid dispersion with a floating layer. One part of this 100 mg/I solution was filtered in order to remove the larger undissolved test substance particles. The filtering resulted in a clear greyish coloured solution. The other part of the 100 mg/I solution was not filtered and used as such. Under the conditions of the present study test material did not induce acute immobilisation of Daphnia magna at a nominal concentration of 100 mg/l after 48 hours of exposure (NOEC).Based on the NOEC value it can be concluded that test material has no hazardous effect on aquatic invertebrate and can not be classified as per CLP criteria.
The above study was supported by another study whose aim of the study was to assess the short term toxicity of test chemical to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs.The stock solution 10.0 g/l was prepared by dissolving dark violet powder in acetone. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample in reconstituted water. It was not possible to test higher substance concentration due to formation of precipitate.0,0,2,3,4,6,10 mg/l concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0.The median effective concentration (EC50) for the test substance,in Daphnia magna was determined to be 19.7 mg/L with 95% CI:13.4 -28.9 on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, substance is likely to be hazardous to aquatic invertebrate and can be classified as aquatic acute/ chronic 3 category as per the CLP classification criteria.
Even though varied results for short term toxicity of aquatic invertebrate were available but, based on first experimental purchased study and maximum notifiers of ECHA , we can consider that the test chemical is non toxic and cannot be classified for aquatic invertebrates.
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