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Diss Factsheets
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EC number: 205-187-4 | CAS number: 135-37-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1971
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Meets generally accepted scientific standards, well documented and acceptable for assessment (pre-OECD /pre-GLP study)
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 971
- Report date:
- 1971
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Rats were exposed for 4 hours to Na3NTA (different concentration).
Animals were observed 14 days after treatment for clinical signs.
Body weight was measured 15 days after treatment and compared to control animals. - GLP compliance:
- no
- Remarks:
- pre-GLP study
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Trisodium nitrilotriacetate
- EC Number:
- 225-768-6
- EC Name:
- Trisodium nitrilotriacetate
- Cas Number:
- 5064-31-3
- Molecular formula:
- C6H9NO6.3Na
- IUPAC Name:
- trisodium 2,2',2''-nitrilotriacetate
- Details on test material:
- - Substance type: pure substance
- Physical state: solid, finely divided powder, pale yellow in colour
- Analytical purity: no data
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Test animals: Sprague Dawley albino rats
- Source: no data
- Weight at study initiation: 225-271 g
Administration / exposure
- Route of administration:
- inhalation: aerosol
- Type of inhalation exposure:
- not specified
- Vehicle:
- air
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure chamber volume: 38 liter glass inhalation chamber
- Method of holding animals in test chamber: no data
- System of generating particulates/aerosols: cyclone dust generator or pulse-puff aerosol generator
- Method of particle size determination: Monsanto Cascade Impactor
TEST ATMOSPHERE
- Brief description of analytical method used: gravimetrical determination of test material concentrations.
- Samples taken from breathing zone: yes, samples of the chamber atmosphere were passed through MSA (Mine safety appliances) glass fiber filters. These filters were subsequently weighed, and the chamber concentration was determined by dividing the total weight of the test material collected on the filter by the volume of air sampled.
TEST ATMOSPHERE (if not tabulated)
Na3NTA (non-micronized) 70 % of the particles smaller that 10 µm, mass median diameter: 6.9 µm.
Na3NTA (micronized) 90 % of the particles smaller that 10 µm, mass median diameter: 4.3 µm. - Analytical verification of test atmosphere concentrations:
- yes
- Remarks:
- gravimetrical measurment
- Duration of exposure:
- ca. 4 h
- Concentrations:
- 3.30, 3.60 and 5.02 mg/l non-micronised NTA
5.033 mg/l micronised NTA - No. of animals per sex per dose:
- 10 male albino rats per dose
- Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes
- Other examinations performed:
- Clinical observation, during exposure and during post exposure period, salivation, slow labored respiration, partially closed eyes, hypoactivity
- Body weight, recorded on days 1, 2, 8, 15
- Gross findings
- No histopathology - Statistics:
- No details described in the study
Results and discussion
Effect levels
- Sex:
- male
- Dose descriptor:
- LC0
- Effect level:
- 5 mg/L air
- Remarks on result:
- other: no deaths at 5.0 mg XTW-448 / l air
- Mortality:
- No mortalities in rats exposed to any of the above mentioned aerosol concentrations neither non-micronized nor micronized
No effect level could be determined up to 5 mg/l air Na3NTA. - Clinical signs:
- other: Group 1 (3.30 mg/l) During the exposure, all rats cleaned themselves in the initial phases. Subsequently, during the first hour of exposure and throughout the remainder of the four hours, the rats exhibited salivation, slow labored respiration, gasping, p
- Body weight:
- In every instance, rats exposed to non-micronized Na3NTA lost weight after the first exposure, but had recorded an average weight increase by Days 8 and 15. Those rats exposed to micronized Na3NTA recorded a substantial average weight gain by Day 15. For details see table 1.
- Gross pathology:
- Necropsy findings: No consistent gross alterations were seen in any of the groups of rats exposed to Na3NTA at varying atmospheric concentrations for a period of four hours.
Generally, all tissues appeared normal when compared to those tissues from the control animals.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Single acute Inhalation exposures of male albino rats to various levels of Na3NTA for four hours did not result in mortalities.
The highest concentration (5 mg/l air) was the maximum level attainable under dynamic exposure conditions utilizing a cyclone dust generator or a pulse-puff generator.
There was no difference in the toxicity of the non-micronized or micronized test material.
Clinical observations of rats exposed to various concentrations of the test material generally included salivation, slow labored respiration, partially
closed eyes and hypoactivity. All animals recovered after the exposures, and appeared normal and healthy during the two-week observation period.
Necropsies of rats from each of the exposure groups did not reveal abnormalities which were grossly different from the control group. - Executive summary:
In an acute inhalation toxicity study groups of 10 male Sprague Dawley albino rats per dose were exposed by inhalation route to Na3NTA for 4 hours at concentrations of 3.3, 3.6, 5.0 mg/l (non-micronised NTA) and 5.0 mg/l (micronised NTA). Animals then were observed for 14 days.
No mortality was observed.
Clinical observations disappered within the observation period of 14 days after treatment.
Body weights were normal compared to the control on day 15 after treatment.
Necropsies revealed no differences to control.
The 4-h LC50-value was greater than the highest measured concentration of 5.0 mg Na3NTA/l air.
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