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EC number: 246-680-4 | CAS number: 25155-30-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- (Q)SAR
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification
- Justification for type of information:
- QSAR prediction: US EPA accepted QSAR method for chemicals properties assessment.
- Qualifier:
- according to guideline
- Guideline:
- other: QSAR model
- Principles of method if other than guideline:
- EPI Suite v 4.0/ECOSARv1.00
- GLP compliance:
- no
- Remarks:
- not applicable. QSAR model
- Analytical monitoring:
- not required
- Vehicle:
- no
- Test organisms (species):
- other: Daphnid species
- Test type:
- other: QSAR model
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- not applicable. QSAR model
- Test temperature:
- not applicable. QSAR model
- pH:
- not applicable. QSAR model
- Dissolved oxygen:
- not applicable. QSAR model
- Salinity:
- not applicable. QSAR model
- Nominal and measured concentrations:
- not applicable. QSAR model
- Details on test conditions:
- 100% Benzenesulfonic acid, dodecyl-, sodium salt
SMILES : O([Na])S(=O)(=O)c1ccc(cc1)CCCCCCCCCCCC
CHEM : Benzenesulfonic acid, dodecyl-, sodium salt
CAS Num: 25155-30-0
MOL FOR: C18 H29 O3 S1 Na1
MOL WT : 348.48
Log Kow: 3.00 (KowWin estimate)
Wat Sol: 800 mg/L (experimental database) - Reference substance (positive control):
- not required
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 23.265 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- The ECOSAR QSAR model predicts that LC50 for fresh water species Daphnid would to be 23.265 mg/l therefore that the substance is slightly toxic to Daphnid.
- Validity criteria fulfilled:
- yes
- Remarks:
- US EPA accepted QSAR method for chemicals properties assessment.
- Conclusions:
- The ECOSAR QSAR model predicts that LC50 for fresh water species Daphnid would to be 23.265 mg/l therefore that the substance is slightly toxic to Daphnid.
- Executive summary:
The ECOSAR QSAR model predicts that LC50 for fresh water species Daphnid would to be 23.265 mg/l therefore that the substance is slightly toxic to Daphnid.
Daphnid , 48-hr LC50 = 23.265 mg/L (ppm)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- (Q)SAR
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification
- Justification for type of information:
- QSAR prediction: US EPA accepted QSAR method for chemicals properties assessment.
- Qualifier:
- according to guideline
- Guideline:
- other: QSAR model
- Principles of method if other than guideline:
- EPI Suite v 4.0/ECOSARv1.00
- GLP compliance:
- no
- Remarks:
- not applicable. QSAR model
- Analytical monitoring:
- not required
- Vehicle:
- no
- Test organisms (species):
- other: Mysid shrimp
- Details on test organisms:
- Scientific name: Americamysis bahia
Common name: Opossum shrimp, Mysid shrimp, Mysids - Test type:
- other: QSAR model
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- not applicable. QSAR model
- Test temperature:
- not applicable. QSAR model
- pH:
- not applicable. QSAR model
- Dissolved oxygen:
- not applicable. QSAR model
- Salinity:
- not applicable. QSAR model
- Nominal and measured concentrations:
- not applicable. QSAR model
- Details on test conditions:
- 100% Benzenesulfonic acid, dodecyl-, sodium salt
SMILES : O([Na])S(=O)(=O)c1ccc(cc1)CCCCCCCCCCCC
CHEM : Benzenesulfonic acid, dodecyl-, sodium salt
CAS Num: 25155-30-0
MOL FOR: C18 H29 O3 S1 Na1
MOL WT : 348.48
Log Kow: 3.00 (KowWin estimate)
Wat Sol: 800 mg/L (experimental database) - Reference substance (positive control):
- not required
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 17.429 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- The ECOSAR QSAR model predicts that LC50 for fresh water species cited would to be 17.429 mg/l therefore that the substance is slightly toxic to Mysid shrimp.
- Validity criteria fulfilled:
- yes
- Remarks:
- US EPA accepted QSAR method for chemicals properties assessment.
- Conclusions:
- The ECOSAR QSAR model predicts that LC50 for fresh water species cited would to be 17.429 mg/l therefore that the substance is slightly toxic to Mysid shrimp.
- Executive summary:
The ECOSAR QSAR model predicts that LC50 for fresh water species cited would to be 17.429 mg/l therefore that the substance is slightly toxic to Mysid shrimp.
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- other: published data
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- Dodecylbenzene sulfonic acids (CAS# 27176-87-0 , EC Number; 248-289-4) ) is a very close analogue of Sodium dodecylbenzenesulfonate (CAS No. 25155-30-0, EC Number; 246-680-4) ) and the dissociated acid it readily dissociates in water and release the dodecylbenzene sulfonic anion in solution
- Qualifier:
- according to guideline
- Guideline:
- other: DIN 38412 Part 11.
- Principles of method if other than guideline:
- All parameters were maintained in accordance with acceptable DIN guidelines.
- GLP compliance:
- not specified
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- SPECIES #
(NAME): 5 Daphnia magna (Water Flea)
AGE: NR NR
LIFE STAGE: not reported, unknown
COMMENT: NR - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 24 h
- Hardness:
- NR
- Test temperature:
- 20 °C
- pH:
- 8.4
- Dissolved oxygen:
- 9.8 mg/l
- Salinity:
- NR
- Nominal and measured concentrations:
- TYPE VALUE RANGES STANDARD CONC (ug/L) IONCIC FRACTION
F 12000 NR TO NR 12000 ug/L NR - Details on test conditions:
- TEST CONDITIONS
STUDY TYPE:
MEDIA: FW (Fresh water)
LOCATION: LAB (Laboratory, indoor)
CONTROL: I (insufficient control)
EXPOSURE TYPE: S ( static)
APPLICATION FREQ.: 1 X
EXPOSURE DUR.: 24 hour(s)
STAND DUR. (D): 1 day(s
TEST CONCENTRATION
CHEM ANAL. METHOD: unmeasured
UNIT OF MEASURE: ug/L micrograms per liter
TYPE VALUE RANGES STANDARD CONC (ug/L) IONCIC FRACTION
F 12000 NR TO NR 12000 ug/L NR
WATER QUALITY VALUE RANGE (UNIT)
TEMPERATURE: 20 NR TO NR C
pH: 8.4 NR TO NR
HARDNESS: NR NR TO NR mg/L CaCO3
SALINITY: NR NR TO NR
ALKALINITY: NR NR TO NR mg/L CaCO3
CONDUCTIVITY: 325 NR TO NR μS/cm
DISSOLVED OXYGEN: 9.8 NR TO NR mg/l
ORGANIC CARBON (NR): NR NR TO NR NR - Reference substance (positive control):
- no
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 12 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC0
- Effect conc.:
- 6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC100
- Effect conc.:
- 14 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- A 24 h EC50 of 12 mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna.
- Validity criteria fulfilled:
- yes
- Conclusions:
- A 24 h EC50 of 12 mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna.
- Executive summary:
A 24 h EC50 of 12 mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna.
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- other: published data
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- Dodecylbenzene sulfonic acids (CAS# 27176-87-0 , EC Number; 248-289-4) ) is a very close analogue of Sodium dodecylbenzenesulfonate (CAS No. 25155-30-0, EC Number; 246-680-4) ) and the dissociated acid it readily dissociates in water and release the dodecylbenzene sulfonic anion in solution
- Qualifier:
- according to guideline
- Guideline:
- other: Directive 84/449/EEC, C.2 "Acute toxicity for Daphnia"
- Principles of method if other than guideline:
- River Po waters were collected at the end of the drainage basin, extracted by means of XAD-2 resins and tested for toxicity on aquatic organisms (Daphnia magna and Selenastrum capricornutum) and for mutagenicity with a modified Ames test. The extracts were analyzed by HRGC/FID and selective detectors for the determination of the most common classes of toxic compounds.
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- SPECIES #
(NAME): 5 Daphnia magna (Water Flea)
AGE: NR NR
LIFE STAGE: not reported, unknown
COMMENT: NR - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- NR
- Test temperature:
- 20 °C
- Salinity:
- NR
- Nominal and measured concentrations:
- TYPE VALUE RANGES STANDARD CONC (ug/L) IONCIC FRACTION
F 5880 NR TO NR 5880 ug/L NR - Details on test conditions:
- TEST CONDITIONS
STUDY TYPE:
MEDIA: FW (Fresh water)
LOCATION: LAB (Laboratory, indoor)
CONTROL: I (insufficient control)
EXPOSURE TYPE: S ( static)
APPLICATION FREQ.: 1 X
EXPOSURE DUR.: 48 hour(s)
STAND DUR. (D): 2 day(s
TEST CONCENTRATION
CHEM ANAL. METHOD: unmeasured
UNIT OF MEASURE: ug/L micrograms per liter
TYPE VALUE RANGES STANDARD CONC (ug/L) IONCIC FRACTION
F 5880 NR TO NR 5880 ug/L NR
- Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 5.88 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- A 48 h EC50 of 5.88 mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna.
- Validity criteria fulfilled:
- yes
- Conclusions:
- A 48 h EC50 of 5.88 mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna.
- Executive summary:
A 48 h EC50 of 5.88 mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna.
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- other: published data
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- C12 LAS; Linear Alkylbenzene Sulfonate (LAS)) is a very close analogue of Sodium dodecylbenzenesulfonate
- Qualifier:
- according to guideline
- Guideline:
- other: EPA-660/3-75-009 1975. Method for acute toxicity tests with fish, macroinvertebrates and amphibians.
- Principles of method if other than guideline:
- Acute toxicity tests were conducted on high molecular weight LAS, individual pure homologues, non-linear LAS components (dialkyl tetralin or indane sulfonates (DTIS), and model biodegradation intermediates (sulfophenyl undecane, SØU) in order to determine whether biodegradation decreases toxicity. In 250 mL beakers with 200 mL of well water of approximately 250 mg/L hardness, ten Daphnia, less than 18 hours old, were placed in each of the three beakers. No food was added for the duration of the test.
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- In 250 mL beakers with 200 mL of well water of approximately 250 mg/L hardness, ten Daphnia, less than 18 hours old, were placed in each of the three replicate beakers per concentration. Five concentrations were tested.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnia magna (Water fleas); <18 hr old
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- open system
- Hardness:
- 250 mg/L
- Test temperature:
- not reported
- pH:
- not reported
- Details on test conditions:
- This test were performed in 250 mL beakers with 200 mL of well water of approximately 250 mg/L hardness. Ten daphnia (<18 hr old) were placed in each of 3 replicate beakers.No food was added.
- Reference substance (positive control):
- not required
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 10.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: C12 -LAS
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 3.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: C12 -LAS
- Details on results:
- A 48 h LC50 of 3.5mg/L has been determined for the effects of the substance C12 -LAS on mortality of the Daphnia magna.
- Validity criteria fulfilled:
- yes
- Conclusions:
- A 48 h LC50 of 3.5mg/L has been determined for the effects of the substance C12 -LAS on mortality of the Daphnia magna.
- Executive summary:
The toxicity of high molecular weight LAS, individual LAS homologues, and nonlinear LAS components (DTIS) was measured in a series of aquatic toxicity tests with Daphnia magna. Results show that biodegradation of LAS influences the toxicity, with the remaining LAS becoming less toxic. The 48 -hour LC50 of high molecular weight LAS to Daphnia magna is 2.4 mg/L. A 48 h LC50 of 3.5 mg/L has been determined for the effects of the substance C12 -LAS on mortality of the Daphnia magna.
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- other: published data
- Adequacy of study:
- weight of evidence
- Study period:
- May 8, 2000
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Species: Daphnia magna, <24 h old.
No.of daphnids: 5/replicate, 4 replicates/treatment. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- hardness 168 mg/l (CaCO3)
- Test temperature:
- 20±2°C
- pH:
- pH 8.4-8.5;
- Dissolved oxygen:
- O2 91-97%;
- Nominal and measured concentrations:
- Concentration Nominal: 1.56, 3.13, 6.25, 12.5, 25, 50 and 100 mg/L
(no vehicle), untreated control - Details on test conditions:
- Test conditions :Static; at 20±2°C in 225 mL crystallising dishes (covered), containing 100 mL of reconstituted water of hardness 168 mg/l (CaCO3) and pH 8.3, 16 h light.
Phys. meas.: At 0 and 48 h in one replicate for all concentrations; overall ranges for pH 8.4-8.5; O2 91-97%; temperature (0, 24 and 48 h) 20-21°C.
Observations: Immobility/mortality at 24 and 48 h. - Reference substance (positive control):
- not required
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: (95% CI 2.2-2.7 mg/L)
- Details on results:
- The 48-h EC50 calculated using trimmed SPK was 2.5 mg/L (95% CI 2.2-2.7 mg/L) ⇔ 2.2 mg a.i./L (95% CI 2.0-2.4 mg a.i./L).
- Validity criteria fulfilled:
- yes
- Conclusions:
- A 48 h EC50 of 2.5mg/L has been determined for the effects of this substance on Immobility of the Daphnia magna.
- Executive summary:
A 48 h EC50 of 2.5 mg/L has been determined for the effects of this substance on Immobility of the Daphnia magna.
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- other: published data
- Adequacy of study:
- weight of evidence
- Study period:
- May 8, 2000
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Species: Daphnia magna, <24 h old.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 20±1 degree C
- pH:
- pH 7.8-8.0
- Dissolved oxygen:
- dissolved oxygen 7.7-8.0;
- Nominal and measured concentrations:
- 0.4, 0.7, 1.2, 2.0, 3.5, 6.0, and 10 mg/L
- Details on test conditions:
- Seven concentrations and a control were tested (0.4, 0.7, 1.2, 2.0, 3.5, 6.0, and 10 mg/L). Physicochemical parameters were maintained within acceptable ranges (temperature 20±1 degree C; dissolved oxygen 7.7-8.0; pH 7.8-8.0). At 48 hours, 0/20 daphnids were immobile in the 0.4 through 3.5 mg/L concentrations, 14/20 in 6.0 mg/L, and 20/20 in 10 mg/L. Graphical interpolation was used to calculate the EC50 value.
- Reference substance (positive control):
- not required
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 5.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: (95% 3.5-6.0 mg/L)
- Details on results:
- A 48 h EC50 of 5.2 mg/L has been determined for the effects of this substance on Immobility of the Daphnia magna.
- Validity criteria fulfilled:
- yes
- Conclusions:
- A 48 h EC50 of 5.2 mg/L has been determined for the effects of this substance on Immobility of the Daphnia magna.
- Executive summary:
A 48 h EC50 of 5.2 mg/L has been determined for the effects of this substance on Immobility of the Daphnia magna.
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- other: published data
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The effects of four concentrations of dodecyl benzene sulfonate at four salinity levels on adult Tisbe bulbisetosa were studied in laboratory experiments. The DBS solutions were analysed by the methylene method to control their stability with time.
The stock solution has been prepared by dissolving 100 mg of pure DBS to 100 mL of distilled water. Test solutions for this test have been obtained both by diluting 3, 6, 9, 12 mL of the stock solution in filtered seawater at a determined level of salinity to 1000 mL, and by diluting the fixed amount stock solution in seawater of 9, 12, 18, 36‰ salinity. - GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- The stock solution has been prepared by dissolving 100 mg of pure DBS to 100 mL of distilled water. Test solutions for this test have been obtained both by diluting 3, 6, 9, 12 mL of the stock solution in filtered seawater at a determined level of salinity to 1000 mL, and by diluting the fixed amount stock solution in seawater of 9, 12, 18, 36‰ salinity.
- Test organisms (species):
- other aquatic crustacea: Tisbe bulbisetosa
- Details on test organisms:
- Tisbe bulbisetosa : adult female
- Test type:
- not specified
- Water media type:
- saltwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Nominal and measured concentrations:
- This study is the combined effects of four concentrations (0, 3, 6, 9, 12 ppm) of DBS at four salinity (9, 12, 18, 360) on adult Tisbe bulbisetosa.
- Reference substance (positive control):
- not required
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 4.58 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: (18‰ salinity) = 4.58 ppm(95% confidence interval: 4.00 to 5.24)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 5.12 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: (36‰ salinity) = 5.12 ppm(95% confidence interval: 4.27 to 6.13)-
- Details on results:
- - 48 hr-LC50 (36‰ salinity) = 5.12 ppm(95% confidence interval: 4.27 to 6.13)- 48 hr-LC50 (18‰ salinity) = 4.58 ppm(95% confidence interval: 4.00 to 5.24)
A 48 h LC50 of 4.58 mg/L (18‰ salinity) and 5.1 mg/L (36‰ salinity) has been determined for the effects of the substance Dodecylbenzenesulfonate (DBS) on mortality of the Tisbe bulbisetosa. - Validity criteria fulfilled:
- yes
- Conclusions:
- A 48 h LC50 of 4.58 mg/L (18‰ salinity) and 5.1 mg/L (36‰ salinity) has been determined for the effects of the substance Dodecylbenzenesulfonate (DBS) on mortality of the Tisbe bulbisetosa.
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- other: published data
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- according to guideline
- Guideline:
- other: EPA-660/3-75-009
- Principles of method if other than guideline:
- Environmnetal Protection Agency (1975) ; EPA-660/3-75-009, Method for acute toxicity tests with fish, macroinertebrates and amphibians
- GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Vehicle:
- no
- Details on test solutions:
- In 250 mL beakers with 200 mL of well water of approximately 250 mg/L hardness, ten Daphnia, less than 18 hours old, were placed in each of the three replicate beakers per concentration. Five concentrations were tested.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnia magna <24 hr old
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 165 mg/L (as CaCO3)
- Test temperature:
- 21-23 deg C
- pH:
- 8.1-8.9
- Dissolved oxygen:
- 8.4 ppm
- Nominal and measured concentrations:
- - five test concentrations and control (nominal)
- Details on test conditions:
- - Dilution water was a reconstituted water.(average Hardness (as CaCO3): 165 mg/L, Alkalinity (as CaCO3): 120 +/- 10 mg/L, Dissolved oxygen 5.3mg/L (>60% saturation), temperature : 22 +/- 1 deg C, pH : 7.9-8.4)- Number of replicates; 3 replicates- light intensity (12hr photoperiod at 40-70 L/ft^2)- The 250 mL glass test chambers contained 200ml of test water and five test Daphnia.- five concentrations and control
- Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 1.8 - 5.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Remarks on result:
- other: C12 -LAS
- Details on results:
- A 48 h LC50 of 1.8 - 5.6 mg/L has been determined for the effects of the substance C12 -LAS on mortality of the Daphnia magna.
- Validity criteria fulfilled:
- yes
- Conclusions:
- A 48 h LC50 of 1.8 - 5.6 mg/L has been determined for the effects of the substance C12 -LAS on mortality of the Daphnia magna.
- Executive summary:
A 48 h LC50 of 1.8 - 5.6 mg/L has been determined for the effects of the substance C12 -LAS on mortality of the Daphnia magna.
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- SPECIES #
(NAME): 5 Daphnia magna
(Water Flea)
AGE: <24 h
LIFE STAGE: neonate
COMMENT: FEMALE,CLONE A STRAIN - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- NR
- Test temperature:
- 21 °C
- pH:
- NR
- Dissolved oxygen:
- NR
- Salinity:
- NR
- Nominal and measured concentrations:
- CHEM ANAL. METHOD: unmeasured
UNIT OF MEASURE: mg/L miligram per liter
TYPE VALUE RANGE STANDARD CONC (ug/L) ION
F 6.3 NR TO NR 6300 ug/L NR
F 7.5 NR TO NR 7500 ug/L NR
F 9.5 NR TO NR 9500 ug/L NR - Details on test conditions:
- TEST CONDITIONS
STUDY TYPE:
MEDIA: FW (Fresh water)
LOCATION: LAB (Laboratory, indoor)
CONTROL: C (sufficient control)
EXPOSURE TYPE: S ( static)
APPLICATION FREQ.: 1 X
EXPOSURE DUR.: 48 hour(s)
STAND DUR. (D): 2 day(s
TEST CONCENTRATION
CHEM ANAL. METHOD: unmeasured
UNIT OF MEASURE: mg/L miligram per liter
TYPE VALUE RANGE STANDARD CONC (ug/L) ION
F 6.3 NR TO NR 6300 ug/L NR
F 7.5 NR TO NR 7500 ug/L NR
F 9.5 NR TO NR 9500 ug/L NR
WATER QUALITY VALUE RANGE (UNIT)
TEMPERATURE: 21 NR TO NR C
pH: NR NR TO NR
HARDNESS: NR NR TO NR mg/L CaCO3
SALINITY: NR NR TO NR
ALKALINITY: NR NR TO NR mg/L CaCO3
CONDUCTIVITY: NR NR TO NR μS/cm
DISSOLVED OXYGEN: NR NR TO NR mg/l
ORGANIC CARBON (NR): NR NR TO NR NR - Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 6.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 7.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 9.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- A 48 h EC50 of 6.3 mg/L has been determined for the effects of the test substance on mortality of the Daphnia magna.
- Validity criteria fulfilled:
- yes
- Conclusions:
- A 48 h EC50 of 6.3 mg/L has been determined for the effects of the test substance on mortality of the Daphnia magna.
- Executive summary:
A 48 h EC50 of 6.3 mg/L has been determined for the effects of the test substance on mortality of the Daphnia magna.
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- SPECIES #
(NAME): 5 Daphnia magna
(Water Flea)
AGE: NR
LIFE STAGE: not reported, unknown
COMMENT: NR - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 24 h
- Hardness:
- NR
- Test temperature:
- NR
- pH:
- NR
- Dissolved oxygen:
- NR
- Salinity:
- NR
- Nominal and measured concentrations:
- CHEM ANAL. METHOD: unmeasured
UNIT OF MEASURE: ug/L micrograms per liter
TYPE VALUE RANGE STANDARD CONC (ug/L) ION
F 12000 NR TO NR 12000 ug/L NR
F 17000 NR TO NR 17000 ug/L NR - Details on test conditions:
- TEST CONDITIONS
STUDY TYPE:
MEDIA: FW (Fresh water)
LOCATION: LAB (Laboratory, indoor)
CONTROL: I (insufficient control)
EXPOSURE TYPE: S ( static)
APPLICATION FREQ.: 1 X
EXPOSURE DUR.: 24 hour(s)
STAND DUR. (D): 1 day(s
TEST CONCENTRATION
CHEM ANAL. METHOD: unmeasured
UNIT OF MEASURE: ug/L micrograms per liter
TYPE VALUE RANGE STANDARD CONC (ug/L) ION
F 12000 NR TO NR 12000 ug/L NR
F 17000 NR TO NR 17000 ug/L NR
WATER QUALITY VALUE RANGE (UNIT)
TEMPERATURE: 21 NR TO NR C
pH: NR NR TO NR
HARDNESS: NR NR TO NR mg/L CaCO3
SALINITY: NR NR TO NR
ALKALINITY: NR NR TO NR mg/L CaCO3
CONDUCTIVITY: NR NR TO NR μS/cm
DISSOLVED OXYGEN: NR NR TO NR mg/l
ORGANIC CARBON (NR): NR NR TO NR NR - Reference substance (positive control):
- no
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 12 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 17 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- A 24 h EC50 of 12mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna.
- Validity criteria fulfilled:
- yes
- Conclusions:
- A 24 h EC50 of 12mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna.
- Executive summary:
A 24 h EC50 of 12 mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna.
Referenceopen allclose all
Overview of QSARs estimations on short-term aquatic toxicity of
Sodium dodecylbenzenesulfonate/Benzenesulfonic acid, dodecyl-, sodium salt
to Daphnid
Method
|
Results |
Remarks |
Reference |
QSAR estimate |
Daphnid , 48-hr LC50 =23.265 mg/L (ppm)
Daphnid, 30-day, ChV (chronic value) = 3.159 mg/L (ppm)
|
Reliable with restrictions, QSAR estimated data |
ECOSAR Program (v1.00) |
Using the EPIWIN ECOSAR QSAR model aLC50 for fresh water Daphnid of 23.265 mg/l was established.
Daphnid , 48-hr LC50 =23.265 mg/L (ppm)
Daphnid, 30-day, ChV (chronic value) = 3.159 mg/L (ppm)
Overview of QSARs estimations on short-term aquatic toxicity of
Sodium dodecylbenzenesulfonate/Benzenesulfonic acid, dodecyl-, sodium salt
to Mysid shrimp
Method
|
Results |
Remarks |
Reference |
QSAR estimate |
Mysid Shrimp, 96 -hr, LC50= 17.429 mg/L (ppm) Mysid Shrimp (SW), 30-day, ChV (chronic value) = 1.121 mg/L (ppm)
|
Reliable with restrictions, QSAR estimated data |
ECOSAR Program (v1.00) |
Mysid Shrimp, 96 -hr, LC50= 17.429 mg/L (ppm)
Mysid Shrimp (SW), 30-day, ChV (chronic value) = 1.121 mg/L (ppm)
Results:The following table shows the acute toxicity of original materials (LC50, mg/L)..
Daphnia magna |
|||
|
Average chain length |
24 hour |
48 hour |
High molecular weight LAS |
13.3 |
2.6 ± 0.1 |
2.3 ± 0.1 |
Individual homologues LAS |
|
|
|
C10 |
10 |
53.1 ± 0.4 |
12.3 ± 2.6 |
C11 |
11 |
15.8 ± 3.0 |
5.7 ± 0.6 |
C12 |
12 |
10.7 ± 1.6 |
3.5 ± 1.0 |
C13 |
13 |
2.7 ± 0.4 |
2.0 ± 0.3 |
C14 |
14 |
1.2 ± 0.2 |
0.7 ± 0.2 |
Nonlinear LAS components (DTIS) |
|
|
|
C10 |
10 |
106.0 ± 27.0 |
98.0 ± 21.3 |
C12 |
12 |
55.1 ± 9.1 |
34.1 ± 5.1 |
C14 |
14 |
12.4 ± 1.4 |
10.0 ± 1.0 |
Model biodegradation intermediates |
|
|
|
C4 (SØ Butyrate) |
4 |
~12,000 |
~6,000 |
C5 (SØ Valerate) |
5 |
~12,000 |
~5,000 |
C11 (SØU) |
11 |
355 ± 150* |
208 ± 85* |
*Subsequent repurification of this sample yielded a product with the same isomeric composition but with LC50values over 1000 mg/L for daphnids (Swisher et al., 1976).
Description of key information
A 48 h EC50 of 6.3 mg/L has been determined for the effects of the test substance on mortality of the Daphnia magna.
A 24 h EC50 of 12 mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna.
A 48 h LC50 of 3.5mg/L has been determined for the effects of the substance C12 -LAS on mortality of the Daphnia magna.
The ECOSAR QSAR model predicts that LC50 for fresh water species Daphnid would to be 23.265 mg/l therefore that the substance is slightly toxic to Daphnid.
The ECOSAR QSAR model predicts that LC50 for fresh water species cited would to be 17.429 mg/l therefore that the substance is slightly toxic to Mysid shrimp.
A 48 h LC50 of 4.58 mg/L (18‰ salinity) and 5.1 mg/L (36‰ salinity) has been determined for the effects of the substance Dodecylbenzenesulfonate (DBS) on mortality of the Tisbe bulbisetosa.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 6.3 mg/L
Marine water invertebrates
Marine water invertebrates
- Effect concentration:
- 4.58 mg/L
Additional information
A 48 h EC50 of 6.3 mg/L has been determined for the effects of the test substance on mortality of the Daphnia magna.
A 24 h EC50 of 12 mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna.
A 48 h LC50 of 3.5mg/L has been determined for the effects of the substance C12 -LAS on mortality of the Daphnia magna.
The ECOSAR QSAR model predicts that LC50 for fresh water species Daphnid would to be 23.265 mg/l therefore that the substance is slightly toxic to Daphnid.
The ECOSAR QSAR model predicts that LC50 for fresh water species cited would to be 17.429 mg/l therefore that the substance is slightly toxic to Mysid shrimp.
A 48 h LC50 of 4.58 mg/L (18‰ salinity) and 5.1 mg/L (36‰ salinity) has been determined for the effects of the substance Dodecylbenzenesulfonate (DBS) on mortality of the Tisbe bulbisetosa.
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