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EC number: 695-187-4 | CAS number: 166524-75-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 8 November 1993 to 13 November 1993
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Study conducted to GLP in compliance with agreed protocols, with no or minor deviations from standard test guidelines and/or minor methodological deficiencies, which do not affect the quality of the relevant results. No analytical determination of test substance concentration.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: 40 CFR Part 797 Subpart B
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 5-ethoxy-2-({5-ethoxy-7-fluoro-[1,2,4]triazolo[1,5-c]pyrimidin-2-yl}disulfanyl)-7-fluoro-[1,2,4]triazolo[1,5-c]pyrimidine
- EC Number:
- 695-187-4
- Cas Number:
- 166524-75-0
- Molecular formula:
- C14H12F2N8O2S2
- IUPAC Name:
- 5-ethoxy-2-({5-ethoxy-7-fluoro-[1,2,4]triazolo[1,5-c]pyrimidin-2-yl}disulfanyl)-7-fluoro-[1,2,4]triazolo[1,5-c]pyrimidine
- Test material form:
- other: solid (unspecified)
- Details on test material:
- - Appearance: light beige solid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The test material was used as received; it was added directly to the test medium.
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Strain: 1648
Stock cultures of this organism were maintained axenically by weekly transfer into sterile algal assay medium (AAM).
- Culturing media and conditions: same as test
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 5 d
Test conditions
- Test temperature:
- 24.1 - 24.7 °C
- pH:
- 7.2 - 7.5 (without algae); 7.6 - 8.4 (with algae)
- Nominal and measured concentrations:
- 0, 15.75, 31.5, 63, 126, 252 and 504 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: sterilised 250 mL borosilicate Erlenmeyer flasks
- Type: closed with Shimadzu closures
- Fill volume: 100 mL (algal test medium)
- Aeration: flasks were continuously shaken at 100 rpm
- Initial cell density: approximately 10 000 cells/mL. Each test flask was dosed with 1 mL algal inoculum.
- No. of vessels per concentration (replicates): three. A counting blank was included with each test concentration (contained growth medium and the appropriate amount of test material but no algae)
- No. of vessels per control (replicates): six
GROWTH MEDIUM
- Standard medium used: yes (Algal Assay Medium) The culture medium used for the definitive test was the same as that used to maintain the stock culture except that the medium used in the toxicity tests did not contain the chelant, Na2EDTA.2H2O
- Preparation of the Algal Assay Medium:
Each stock solution was filtered through a 0.45 µm membrane filter, then stored at 4 °C; the solutions were brought up to room temperature before using. 1 mL of each stock solution A, B1, B2, B3 and D, plus 10 mL of C were added to 900 mL of dilution water. The mixture was then made up to 1 L with additional water.
A: 12.75 g NaNO3, 6.08 g MgCl2.6H2O and 2.20 g CaCl2.2H2O was added to 500 mL dilution water
B1: 7.35 g MgSO4.7H2O dissolved in 500 mL dilution water
B2: 7.5 g NaHCO3 dissolved in 500 mL dilution water
B3: 0.522 g K2PO4 dissolved in 500 mL dilution water
C: 5 mL of number 1 (below) and 5 mL of number 3 (below) diluted to 500 mL with sterile dilution water.
1: 1.86 g H3BO3, 4.17 g MnCl2.4H2O, 0.0327 g ZnCl2 and 0.0726 g NaMoO4.2H2O dissolved in 1000 mL dilution water
2: 2.86 g CoCl2.6H2O and 0.22 g CuCl2.2H2O dissolved in 1000 mL dilution water
3: 2.5 mL of number 2 was made up to 500 mL
D: 0.30 g Na2EDTA.2H2O and 0.16 g FeCl3.6H2O dissolved in 1000 mL dilution water
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Photoperiod: continuous
- Light intensity and quality: 3800 - 5050 lux
EFFECT PARAMETERS MEASURED
The algal densities of the inoculum and test cultures were determined by electron particle counting using a Coulter Multisizer. Total cell counts were determined at approximately 24, 48, 72, 96 and 120 hours. Cells were cumulatively counted from a lower threshold equivalent spherical diameter of approximately 2.7 µm to a higher threshold equivalent spherical diameter of approximately 8.6 µm. The background particle count (growth medium and test material) was concurrently subtracted from that of the sample dilution (growth medium, test material and algae) to obtain cell density. A blank correction for the isotonic saline solution used in preparing the dilutions was also compensated for in the calculations.
PHYSICAL ANALYSIS
Extra flasks without algae were added to the test for each nominal concentration to monitor pH at the initiation of the test and every day thereafter. At the end of the test a final pH was taken in a replicate test flask at each test concentration with algae. The temperature of a representative test solution was continuously monitored during the test. The light intensity was checked on a daily basis with readings taken corresponding to the positions of the test flasks in the incubator.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: test concentrations were set in a geometric series with a progression factor of 2.0
- Range finding study: yes
- Test concentrations: 0.1, 1.0, 10, 100 and 1000 mg/L
- Results used to determine the conditions for the definitive study: yes; the results indicated essentially no effect at any concentration in comparison to the controls. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 259 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 0.0 - 534.8 mg/L (95 % CL)
- Duration:
- 120 h
- Dose descriptor:
- EC50
- Effect conc.:
- 364 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 175.4 - 552.7 mg/L (95 % CL)
- Duration:
- 120 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 126 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- During the first three days of the study, the data for the 252 mg/L concentration showed no algal growth. This was probably due to the fact that the test material was insoluble at concentrations above 126 mg/L. At the 252 mg/L concentration, the precipitate particle size appeared to be in the same range as the algal cell size and therefore produced a large background count. As the algal growth exceeded the background count, the effects of the test material particle size was reversed and a positive algal growth rate was observed.
The 72 and 120 hour EC50 values (and their 95 % confidence intervals) for algal growth were 259 (0.0 - 534.8) and 364 (175.4 - 552.7) mg/L, respectively, and for percent inhibition were 310 (5.1 - ∞) and 140 (18.1 - 1080.3) mg/L, respectively. The 120 hour NOEC for algal growth was 126 mg/L. - Reported statistics and error estimates:
- The EC10, EC50 and EC90 values for algal growth were numerically derived based on calculated regression equations for each 24 hour period. The no observed effect concentrations (NOECs) for algal growth were calculated using analysis of variance and the Dunnett's test. The percent inhibition values were calculated by regression based on the comparison of the areas under the growth curves between the control group and each exposure concentration.
Any other information on results incl. tables
Table 1: Mean Values for Growth for Each Replicate Flask
Nominal Conc. (mg/L) |
Day 1 |
Day 2 |
Day 3 |
Day 4 |
Day 5 |
0 (control) |
21840 |
67615 |
371400 |
918800 |
2009333 |
20007 |
72099 |
298333 |
895733 |
2274933 |
|
25927 |
72099 |
285867 |
946133 |
2540933 |
|
23733 |
67691 |
285400 |
822200 |
2178533 |
|
24933 |
72960 |
318267 |
894800 |
2236533 |
|
21647 |
80231 |
357933 |
779467 |
2109200 |
|
15.8 |
40787 |
89072 |
306067 |
826400 |
2254533 |
37320 |
88971 |
284000 |
855200 |
2099467 |
|
29927 |
96596 |
285800 |
910933 |
2052667 |
|
31.5 |
26627 |
79065 |
274067 |
839733 |
2154933 |
25007 |
80180 |
247000 |
855600 |
1870267 |
|
29953 |
144957 |
327400 |
1161200 |
2693467 |
|
63 |
39100 |
60749 |
433000 |
1076533 |
3102400 |
30133 |
75721 |
308800 |
749733 |
2152933 |
|
32993 |
89908 |
293000 |
809333 |
2113333 |
|
126 |
53640 |
50971 |
336333 |
784400 |
1899600 |
45373 |
50236 |
283067 |
720400 |
1955200 |
|
40393 |
75164 |
330267 |
789200 |
2306667 |
|
252 |
0 |
0 |
43067 |
604000 |
1543333 |
0 |
0 |
5067 |
669467 |
1546400 |
|
0 |
0 |
0 |
788133 |
1701200 |
|
504 |
61613 |
95481 |
24867 |
120933 |
583467 |
48553 |
0* |
0 |
94133 |
457867 |
|
73807 |
60065 |
146467 |
216667 |
511600 |
* All negative numbers in the data were reset to zero for statistical purposes
Table 2: Results Based on Growth Rate
Time point |
Results |
EC10 (mg/L) |
EC50 (mg/L) |
EC90 (mg/L) |
NOEC (mg/L) |
24 hours |
Total cell count (cells/mL) |
0 |
0 |
0 |
126 |
95% CL |
(0.0 - 745.1) |
(0.0 - 616.4) |
(0.0 - 529.1) |
||
48 hours |
Total cell count (cells/mL) |
82 |
316 |
1089 |
126 |
95% CL |
(3.2 - 2085.7) |
(11.3 - 8820.3) |
(29.9 - ∞) |
||
72 hours |
Total cell count (cells/mL) |
53 |
259 |
458 |
126 |
95% CL |
(0.0 - 326.1) |
(0.0 - 534.8) |
(163.9 - 751.1) |
||
96 hours |
Total cell count (cells/mL) |
101 |
346 |
573 |
126 |
95% CL |
(0.0 - 265.2) |
(175.6 - 515.8) |
(385.8 - 760.3) |
||
120 hours |
Total cell count (cells/mL) |
103 |
364 |
606 |
126 |
95% CL |
(0.0 - 284.3) |
(175.4 - 552.7) |
(395.8 - 815.8) |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of this study, the 72 and 120 hour EC50 values (and their 95 % confidence intervals) for algal growth were 259 (0.0 - 534.8) and 364 (175.4 - 552.7) mg/L, respectively, and for percent inhibition were 310 (5.1 - ∞) and 140 (18.1 - 1080.3) mg/L, respectively. The 120 hour NOEC for algal growth was 126 mg/L.
- Executive summary:
The toxicity of the test material to aquatic algae was investigated in a study which was conducted under GLP conditions and in accordance with the standardised guidelines OECD 201 and 40 CFR Part 797 Subpart B.
During the study, the freshwater green alga Selenastrum capricornutum Printz was exposed to the test material, at nominal concentrations of 0 (control), 15.75, 31.5, 63, 126, 252 and 504 mg/L, for 5 days. The EC50 values, together with the NOEC, were based on nominal concentrations.
Under the conditions of this study, the 72 and 120 hour EC50 values (and their 95 % confidence intervals) for algal growth were 259 (0.0 - 534.8) and 364 (175.4 - 552.7) mg/L, respectively, and for percent inhibition were 310 (5.1 - ∞) and 140 (18.1 - 1080.3) mg/L, respectively. The 120 hour NOEC for algal growth was 126 mg/L. Results are based on nominal test concentrations.
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