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EC number: 200-143-0 | CAS number: 52-51-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28 Aug 2000 - 8 Nov 2000
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2001
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.5 (Acute Toxicity: Eye Irritation / Corrosion)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Bronopol
- EC Number:
- 200-143-0
- EC Name:
- Bronopol
- Cas Number:
- 52-51-7
- Molecular formula:
- C3H6BrNO4
- IUPAC Name:
- 2-bromo-2-nitropropane-1,3-diol
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- Himalayan
- Details on test animals or tissues and environmental conditions:
- Stock/Breeder: LPT Laboratory of Pharmacology and Toxicology KG, branch Lohndorf, D-24601 Lohndorf/Post Wankendorf
Sex: male
Age: 8.5-9mos
Weight: 2.7 kg
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- no
- Amount / concentration applied:
- 100 mg
- Duration of treatment / exposure:
- single exposure
- Observation period (in vivo):
- Examined at 60 min, 1, 24, 48, 72 hours, daily from day 4-21
- Number of animals or in vitro replicates:
- 1
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #1
- Time point:
- other: 1 h
- Score:
- 4
- Max. score:
- 4
- Reversibility:
- other: further assessment not possible
- Irritation parameter:
- iris score
- Basis:
- animal #1
- Time point:
- other: 1 h
- Score:
- 2
- Max. score:
- 2
- Reversibility:
- other: further assessment not possible
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 1.3
- Max. score:
- 2
- Reversibility:
- not reversible
- Irritation parameter:
- chemosis score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 3.6
- Max. score:
- 4
- Reversibility:
- not reversible
- Irritant / corrosive response data:
- Cornea: Grade 4 opacity was observed at 1 hour after application. Further assessment was not possible: whitish deposits (probably pus) were seen from 72 hours and eyelids sticking together from day 5 after application onwards. The cornea was completely destroyed by day 18 after treatment.
Iris: Grade 2 irritation of the iris was observed at 1 hour after application. Further assessment of the iris was not possible.
Conjunctiva:
Redness: Grade 1-2 conjunctival redness was seen during the complete observation period except for days 5-17, where no assessment was possible.
Chemosis: Grade 3-4 conjunctival chemosis was seen between 1 hour and 18 days after application. Afterwards grade 2 chemosis was observed. - Other effects:
- Systemic intolerance reactions were not observed.
Any other information on results incl. tables
|
Cornea |
Iris |
Conjunctiva |
|
redness |
chemosis |
|||
score (average of animals investigated) |
0 to 4 |
0 to 2 |
0 to 3 |
0 to4 |
60 min |
4 |
2 |
2 |
3 |
24 h |
n.p. |
n.p. |
1 |
3 |
48 h |
n.p. |
n.p. |
1 |
3 |
72 h |
n.p. |
n.p. |
2 |
4 |
Average 24h, 48h, 72h |
n.p. |
n.p. |
1.3 |
3.3 |
Day 21 |
Cornea destroyed |
- |
1 |
2 |
Area effected |
Not reported |
|||
Maximum average score (including area affected, max 110) |
- |
- |
- |
- |
Reversibility* |
n |
n |
n |
n |
average time for reversion |
- |
- |
- |
- |
* c : completely reversible |
- |
- |
- |
- |
n.p. = assessment not possible
Applicant's summary and conclusion
- Interpretation of results:
- Category 1 (irreversible effects on the eye) based on GHS criteria
- Conclusions:
- Reactions in the cornea, iris and the conjunctiva revealed severe irritation of the eye. The cornea was completely destroyed by day 18 after treatment. Eye reactions were not reversible within 21 days after application.
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