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EC number: 269-505-3 | CAS number: 68259-02-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- Principles of method if other than guideline:
- Guidelines followed
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- None
- Analytical monitoring:
- yes
- Details on sampling:
- The concentration of the test substance at the beginning and at the end of the test was analysed. All test medium samples for the analytical determinations were taken after mixing of the test solutions.
- Vehicle:
- no
- Details on test solutions:
- Test concentrations: Based on a pretest, the test concentrations were chosen as 58, 100 and 180 mg/L nominal.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- The daphnids were bred in the laboratories of the Textile Dyes Division, Product Ecology TF under standardized conditions. For the test, animals with age of less than 24 h were used. The quality of the daphnids will be checked at least every 3 months by determining the EC50 values (24 h) for potassium dichromate (range: 0.8-1.5 mg/L). For each concentration, 20 Daphnia magna were used.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- None
- Post exposure observation period:
- No data
- Hardness:
- No data
- Test temperature:
- 21.2 °C
- pH:
- 8.0
- Dissolved oxygen:
- 97-98 %
- Salinity:
- No data
- Nominal and measured concentrations:
- Nominal: 0, 58, 100 and 180 mg/L
Measured: 0, 63, 107.9 and 193.7 mg/L - Details on test conditions:
- Experimental conditions: 48 h at 21 °C, 16 h light/8 h dark
The daphnids were exposed to the test substance in 250 mL beakers containing 100 - 200 mL test medium with 10 animals per beaker. All tests are running in duplicate. During exposure the animals were kept at 21±1°C with 16 h illumination. - Reference substance (positive control):
- yes
- Remarks:
- (potassium dichromate)
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 183.1 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 63 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Details on results:
- The reported results are related to the analytical concentrations of the test substance. During test duration the test substance concentrations in the analysed test media were in the range of 106.3 - 109.3 % of the nominal values. Therefore under the test conditions the test substance was sufficiently stable. However, in all test concentrations a sedimentation of the test substance after 48 h was observed. In the control no immobilization of the daphnids was observed after 48 h. Similarly no immolization was observed at 58 mg/L test concentration, while 10 and 50% immobilization was observed at 100 and 180 mg/L concentrations respectively. Based on these findings, the EC50 value was calculated to be 183.1 mg/L. The EC100 value was clearly higher than 193.7 mg/L.
- Results with reference substance (positive control):
- No data
- Reported statistics and error estimates:
- No data
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48 h EC50 of the test substance to Daphnia magna for immobilisation was 183.1 mg/L (measured concentration).
- Executive summary:
The acute toxicity of the test substance (of 100 % purity) to Daphnia magna was determined in a 48-h static test according to OECD Guideline 202 and EU Method C.2.
Daphnia magna were exposed to nominal concentrations of the test substance (0, 58, 100 and 180 mg/L) for 48-h in static test conditions at 21 °C. During test duration the test substance concentrations in the analyzed test media were in the range of 106.3 - 109.3 % of the nominal values. Therefore, under test conditions the test substance was sufficiently stable.
In the control no immobilization of the daphnids was observed after 48 h. Similarly, no immobilization was observed at 58 mg/L test concentration, while 10 and 50 % immobilization was observed at 100 and 180 mg/L concentrations respectively. Based on these findings, the EC50 value was calculated to be 183.1 mg/L. The EC100 value was clearly higher than 193.7 mg/L.
Reference
Analytical results of the test substance:
Concentration start |
Concentration end |
||||
Nominal concentration mg/L |
mg/L |
% of nominal |
mg/L |
% of nominal |
Average concentration mg/L |
58 |
62.5 |
107.8 |
63.4 |
109.3 |
63.0 |
100 |
106.5 |
106.5 |
109.3 |
109.3 |
107.9 |
180 |
191.4 |
106.3 |
196.0 |
108.9 |
193.7 |
Description of key information
FAT 40171/D had no acute toxic effects on Daphnia magna with a 48-hour EC50 of 183.1 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 183.1 mg/L
Additional information
The acute toxicity of Reactive Black 039 (of 100 % purity) to Daphnia magna was determined in a 48 h static test according to OECD Guideline 202 and EU Method C.2.
Daphnia magna were exposed to nominal concentrations of the test substance (0, 58, 100 and 180 mg/L) for 48 h in static test conditions at 21 °C. During test duration the test substance concentrations in the analyzed test media were in the range of 106.3 - 109.3 % of the nominal values. Therefore, under test conditions the test substance was sufficiently stable.
In the control no immobilization of the daphnids was observed after 48 h. Similarly no immolization was observed at 58 mg/L test concentration, while 10 and 50 % immobilization was observed at 100 and 180 mg/L concentrations respectively. Based on these findings, the EC50 value was calculated to be 183.1 mg/L. The EC100 value was clearly higher than 193.7 mg/L.
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