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EC number: 229-662-0 | CAS number: 6642-31-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Hydrolysis
Administrative data
Link to relevant study record(s)
- Endpoint:
- hydrolysis
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2002-07-01 - 2002-08-22
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- under GLP
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 111 (Hydrolysis as a Function of pH)
- Version / remarks:
- OECD Guideline for Testing of Chemicals No. 111, "Hydrolysis as a Function of pH"; adopted May 12, 1981. (only at pH 4.0, pH 7.0 and pH 9.0)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.7 (Degradation: Abiotic Degradation: Hydrolysis as a Function of pH)
- Version / remarks:
- EEC Directive 92/69, Section C.7, "Abiotic Degradation: Hydrolysis as a Function of pH", L383 A, December 1992.
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: At room temperature (at about 20 °C) - Radiolabelling:
- no
- Analytical monitoring:
- yes
- Details on sampling:
- After incubation 20 µl aliquots of the test solutions at each pH value were analysed without dilution by measuring the UV/VIS signal of 1,3-Dimethyl-4-aminouracil after HPLC separation of the injected sample solution.
- Buffers:
- - pH: 4, 7, 9
- Type and final molarity of buffer:
Buffer pH 4, Biphthalate, Baker, Art. No. 5657
Buffer pH 7, Phosphate, Baker, Art. No. 5656
Buffer pH 9, Borate, Baker, Art. No. 7145
The buffer solutions were sterilized for 25 minutes in an autoclave prior to first use. Nitrogen was passed through the buffer solutions for 3 minutes except when freshly sterilized. - Details on test conditions:
- TEST SYSTEM
- Sterilisation method: All glassware, which must be inert in the pH range applied, was rinsed with sterile buffer. The hydrolysis was carried out in flasks, which were stoppered or sealed with an inert material (e. g. PTFE).
TEST DESIGN
Method
The study was conducted as described in the guidelines.
The test item was dissolved in the buffer solutions and incubated at 50 °C with the water bath kept constant at 土 0.1 °C, usually. The concentration of the test item was determined as a function of time at each pH.
Performance of the Test
A preliminary test was performed at 50 °C ±0.1 °C at each of pH 4.0, pH 7.0 and pH 9.0. Aliquots of each test solution were analysed in time intervals using the analytical method as described later in this report. Analysis was performed before incubation, after 2.4 hours, 24 hours, 48 hours and after 120 hours.
Preparation of the Test Solutions
pH 4.0
A 10.95 mg sample of 1,3-Dimethyl-4-aminouracil was dissolved in 100 ml buffer solution (pH 4.0) to prepare a test solution of 109.5 µg/ml 1,3-Dimethyl-4-aminouracil. This mixture was ultrasonificated for 3 minutes and submitted to a 0.45 µm filtration. Two aliquots of this test solution of approximately 50 ml each were transferred into 50 ml Erlenmeyer flasks in order to perform a duplicate test.
pH 7.0
A 13.48 mg sample of 1,3-Dimethyl-4-aminouracil was dissolved in 100 ml buffer solution (pH 7.0) to prepare a test solution of 134.8 µg/ml 1,3-Dimethyl-4-aminouracil. This mixture was ultrasonificated for 3 minutes and submitted to a 0.45 µm filtration. Two aliquots of this test solution of approximately 50 ml each were transferred into 50 ml Erlenmeyer flasks in order to perform a duplicate test.
pH 9.0
A 11.17 mg sample of 1,3-Dimethyl-4-aminouracil was dissolved in 100 ml buffer solution (pH 9.0) to prepare a test solution of 111.7 µg/ml 1,3-Dimethyl-4-aminouracil. This mixture was ultrasonificated for 3 minutes and submitted to a 0.45 µm filtration. Two aliquots of this test solution of approximately 50 ml each were transferred into 50 ml Erlenmeyer flasks in order to perform a duplicate test. - Duration:
- 5 d
- pH:
- 4
- Temp.:
- 50 °C
- Initial conc. measured:
- >= 111.6 - <= 116.5 mg/L
- Remarks:
- duplicate determination
- Duration:
- 5 d
- pH:
- 7
- Temp.:
- 50 °C
- Initial conc. measured:
- >= 131.3 - <= 140.1 mg/L
- Remarks:
- duplicate determination
- Duration:
- 5 d
- pH:
- 9
- Temp.:
- 50 °C
- Initial conc. measured:
- >= 140.8 - <= 141.5 mg/L
- Remarks:
- duplicate determination
- Number of replicates:
- duplicate determination
- Positive controls:
- no
- Negative controls:
- no
- Preliminary study:
- The results of pH 4.0, pH 7.0 and pH 9.0 showed no significant degradation of 1,3-Dimethyl-4-aminouracil at 50 °C after 5 days.
- Transformation products:
- no
- Key result
- pH:
- 4
- Temp.:
- 50 °C
- Remarks on result:
- hydrolytically stable based on preliminary test
- Key result
- pH:
- 7
- Temp.:
- 50 °C
- Remarks on result:
- hydrolytically stable based on preliminary test
- Key result
- pH:
- 9
- Temp.:
- 50 °C
- Remarks on result:
- hydrolytically stable based on preliminary test
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The study was conducted according to OECD 111 under GLP and is well documented. Hence, the results can be considered as sufficiently reliable to asses the susceptibility towards hydrolysis of 1,3-Dimethyl-4-aminouracil. The results of pH 4.0, pH 7.0 and pH 9.0 showed no significant degradation of 1,3-Dimethyl-4-aminouracil at 50 °C. The degradation of 1,3-Dimethyl-4-aminouracil was less than 10 % after 5 days. According to the EEC Directive 92/69, Section C.7, it can be concluded, that the estimated half-life time is higher than one year under representative environmental conditions (25°C). Therefore, 1,3-Dimethyl-4-aminouracil was considered to be hydrolytically stable, and no further testing was necessary.
- Executive summary:
The hydrolysis determination of 1,3-Dimethyl-4-aminouracil under GLP at different pH values was based on the OECD Guideline No. 111,"Hydrolysis as a Function of pH"; adopted May 12, 1981 and on the EEC Directive 92/69, Section C.7, "Abiotic Degradation: Hydrolysis as a Function of pH",L383 A, December 1992.
The results of pH 4.0, pH 7.0 and pH 9.0 showed no significant degradation of 1,3-Dimethyl-4-aminouracil at 50 °C. The degradation of 1,3-Dimethyl-4-aminouracil was less than 10 % after 5 days. These results were summarised in the table below.
pH
Hydrolysis after 5 days, 50 °C [%]
4.0
< 10
7.0
< 10
9.0
< 10
According to the EEC Directive 92/69, Section C.7 it can be concluded, that the estimated half-life time is higher than one year under representative environmental conditions (25°C). Therefore, 1,3-Dimethyl-4-aminouracil was considered to be hydrolytically stable, and no further testing was necessary.
Reference
Description of key information
Hydrolysis: hydrolytically stable, at pH 4.0, pH 7.0 and pH 9.0 at 50°C no significant degradation observed after 5 days (OECD 111, GLP)
Key value for chemical safety assessment
Additional information
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