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Diss Factsheets
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EC number: - | CAS number: 1474044-66-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 29 April - 03 May, 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): Bio-Terge PAS 7S-DRY
- Analytical purity: 100 % (UVCB)
- Lot/batch No.: PG12122011 (dried from industrial lot 7476775)
- Expiration date of the lot/batch: 12 December 2013
- Storage condition of test material: Controlled Room Temperature (15-25°C, below 70 RH%) - Analytical monitoring:
- yes
- Details on sampling:
- Test concentrations were analytically determined at the start and at the end of the test.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
Prior to treatment, a test solution of 100 mg/L was prepared by diluting an appropriate amount of test item in aquarium water. As only limit test was carried out, further dilution of stock solution was not performed. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebrafish
- Source: Ágnes Németh (self-employed), Veszprém, Hungary
- Length at study initiation: 2.7 – 3.4 cm
- Method of breeding: Fish were bred in a well-known fish farm, under disease- and parasite-controlled conditions.
- Feeding during test: No
ACCLIMATION
- Acclimation period: >12 days
- Health during acclimation (any mortality observed): No mortality for seven days before the test. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Post exposure observation period:
- none
- Hardness:
- The hardness of the dilution water was determined as 54 mg/L (as CaCO3).
- Test temperature:
- The test temperature was between 22.6 and 23.5 °C.
- pH:
- The pH was in the range of 7.85 – 8.10.
- Dissolved oxygen:
- The dissolved oxygen concentration was in the range of 75 – 98 % of the air saturation value at the temperature used.
- Salinity:
- not applicable
- Nominal and measured concentrations:
- nominal: 100 mg/L
geometric mean test item concentration: 94.2 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Aquarium
- Volume 5L
- Aeration: no data
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: < 1.0 g fish/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: circulated and filtered tap water
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light and 8 hours darkness
- Light intensity: no data
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : The number of mortalities and any sub-lethal effect were determined 3, 6, 24, 48, 72 and 96 hours after the start of exposure.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: not applicable - limit test
- Range finding study
- Test concentrations: 0.1, 1, 10, 100 mg/L
- Results used to determine the conditions for the definitive study:
Results of the Preliminary Range-Finding Test
Nominal concentrations
[mg/L] Untreated control 0.1 1 10 100
Number of treated animals 4 4 4 4 4
Number of dead animals 0 0 0 0 0 - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: none
- Observations on body length and weight: no differences observed between test groups and controls
- Other biological observations:No sub-lethal effects were observed in the control and test group for a period of 96 hours
- Mortality of control: None
- Other adverse effects control: None
- Abnormal responses: None
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None
- Effect concentrations exceeding solubility of substance in test medium: no - Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- Not applicable - limit test
- Sublethal observations / clinical signs:
As the measured concentrations deviated not more than 20 per cent from the nominal in all cases, biological results are based on the nominal concentrations.
Cumulative mortality data in the definitive test
Test Group
Mortality rate of treated fish (Dead fish/Treated fish)
3h
6h
24h
48h
72h
96h
Control
0/7
0/7
0/7
0/7
0/7
0/7
100 mg/L (nominal)
0/7
0/7
0/7
0/7
0/7
0/7
- Validity criteria fulfilled:
- yes
- Executive summary:
The acute toxicity of the test item to fish as assessed in a study performed according to OECD TG 203 over a period of 96 hours in a static test system using Danio rerio.
Based on the results of a preliminary study, the test was performed as a limit test using a test concentration of 100 mg/L and one control group. The test concentration was analytically determined at the start and end of the experiment. The geometric mean test item concentration was 94.2 mg/L. As the measured concentrations deviated not more than 20 per cent from the nominal in all cases, biological results are based on the nominal test item concentration.
Seven fish were used in each of the test and control groups. There were no deaths observed in either group during the study. No sub-lethal effects were observed in either group and no differences in body length and weight were observed between the groups.
All validity criteria were met during the study.
Under the conditions of this study the 96-h toxicity to Danio rerio was > 100 mg/L.
Reference
Description of key information
Acute toxicity to fish: 96-h LC50 > 100 mg/L (Danio rerio; OECD TG 203)
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- EC50
- Effect concentration:
- > 100 mg/L
Additional information
The acute toxicity of the test item to fish as assessed in a study performed according to OECD TG 203 over a period of 96 hours in a static test system using Danio rerio (Citoxlab, 2013).
Based on the results of a preliminary study, the test was performed as a limit test using a test concentration of 100 mg/L and one control group. The test concentration was analytically determined at the start and end of the experiment. The geometric mean test item concentration was 94.2 mg/L. As the measured concentrations deviated not more than 20 per cent from the nominal in all cases, biological results are based on the nominal test item concentration.
Seven fish were used in each of the test and control groups. There were no deaths observed in either group during the study. No sub-lethal effects were observed in either group and no differences in body length and weight were observed between the groups.
All validity criteria were met during the study.
Under the conditions of this study the 96-h toxicity to Danio rerio was > 100 mg/L.
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