Octane

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

01 August - 15 September 1983

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study meets generally accepted scientific principles, acceptable for assessment.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1983

Report date:

1983

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Deviations:

yes

Remarks:

very limited documentation

GLP compliance:

not specified

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Octane

EC Number:

203-892-1

EC Name:

Octane

Cas Number:

111-65-9

Molecular formula:

C8H18

IUPAC Name:

octane

Details on test material:

- Name of test material (as cited in study report): n-Octane
- Analytical purity: 100% pure commercial product

Test animals

Species:

rat

Strain:

not specified

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: male (mean): 291 g, female (mean): 217.4 g

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

not specified

Vehicle:

other: unchanged (no vehicle)

Analytical verification of test atmosphere concentrations:

yes

Remarks:

THC

Duration of exposure:

4 h

Concentrations:

24.88 mg/L (nominal)
99.5 ± 7.49 mg/L (re-calculated from 21300 ± 1604.23 ppm (mean measured)

No. of animals per sex per dose:

5 males, 5 females

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: daily
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels

Effect levels 1

Key result

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 24.88 mg/L air (nominal)

Exp. duration:

4 h

Effect levels 2

Key result

Sex:

male/female

Dose descriptor:

LC0

Effect level:

>= 24.88 mg/L air (nominal)

Exp. duration:

4 h

Mortality:

none

Clinical signs:

other: After 15 min of exposure all animals displayed rapid breathing. At 30 min of exposure one male and all females showed tremors and hyperactivity. At one hour these animals became languid with tremors. All animals then were noted to be inactive with rapid r

Body weight:

mean body weight males: 302.2 g (Day 2 postexposure), 309.4 g (Day 3), 317.8 g (Day 4), 331.4 g (Day 7), 347 g (Day 14)
mean body weight females: 213.6 g (Day 2 postexposure), 215.6 g (Day 3), 221.4 g (Day 4), 227.4 g (Day 7), 232.8 g (Day 14)

Gross pathology:

All animals appeared normal at the terminal sacrifice with the exception of one male noted to have a small left testis and one male observed to have a dilated right renal pelvis filled with clear fluid.

Applicant's summary and conclusion

Interpretation of results:

other: Not classified

Remarks:

Criteria used for interpretation of results: other: GHS, EU, 2007

Conclusions:

According to the study design N-octane can be considered to be non-toxic.

Executive summary:

According to the study design N-octane can be considered to be non-toxic.