Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 260-124-8 | CAS number: 56358-09-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 7-12.1.2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- yes
- Remarks:
- see below
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- see below
- Principles of method if other than guideline:
- The study was performed without analytical determination of the test substance concentrations. For the analytical determination of the test substance concentration, no suitable analytical method was found. Therefore for all evaluations and results, nominal concentrations of the test substance were used.
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION OF TEST SOLUTION (especially for difficult test substances)
Since the test substance is poorly soluble in dilution water at concentrations needed for the test, saturated solutions of the test substance in dilution water were prepared by adding measured amounts of the test substance to the dilution water. The solutions were stirred for period of 72 hours on a shaft stirrer and consequently filtered through 0.45 μm filter. These solutions were used for testing. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Strain: Straus
- Source: In house
- Age at study initiation: Young daphnia <24 hours old
- Method of breeding: aperiodic parthenogenesis
- Feeding during test: No
ACCLIMATION
- Acclimation period: Not specified. Before the test, adult gravid females are separated into dilution water. Young daphnia born in the subsequent 24 hours are segregated to handling containers prior to transfer to test vessels.
- Acclimation conditions (same as test or not): Same as test
- Type and amount of food: A mixture of algae - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- Content of (Ca + Mg) in dilution water: 2.49 mmol/L
- Test temperature:
- 20 ± 2 °C
- pH:
- 7.9
- Dissolved oxygen:
- 8.6 mg/L
- Conductivity:
- 0.188 mS/m
- Nominal and measured concentrations:
- - Nominal concentrations: 100, 60, 40, 20 and 10 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Beakers
- Material, size, headspace, fill volume: 50 mL per beaker
- Aeration: No
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- Biomass loading rate: At least 2 mL of test solution per individual
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Standard dilution water was prepared just before the test by admixing 2.5 mL of stored solutions A, B, C and D in 1000 mL of deionised water. Solutions A to D contained 117.6 g CaCl2.2H2O, 49.3 g MgSO4.7H2O, 25.9 g NaHCO3 and 2.3 g KCl, respectively, each in 1 L of deionised water.
- Intervals of water quality measurement: The pH, temperature and dissolved oxygen were measured at the beginning and the end of the test.
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: Natural variation of daily light and dark
- Light intensity: Natural
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
The daphnia immobilisation was observed after 24 and 48 hours.
VEHICLE CONTROL PERFORMED: No required
RANGE-FINDING STUDY
- Test concentrations: 100, 60, 40, 20 and 10 mg/L
- Results used to determine the conditions for the definitive study: No definitive study was carried out. Based on a lack of toxicity of the test substance in the preliminary test, the study was terminated. The concentration of 100 mg/L was included in the preliminary test with 20 daphnids; therefore the conditions of the limit test have been fulfilled. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- There was no immobilisation observed in any dose group throughout the study.
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- EC50: 48 hour EC50 = 0.78 mg/L (95 % confidence limit: 0.64 to 0.93 mg/L)
- Other: The immobilisation of test organisms by the reference substance obtained from the most recent testing facility reference was in the appropriate range. The sensitivity of test species and proficiency of the laboratory in test performance was therefore verified. - Reported statistics and error estimates:
- No immobilisation was observed in the preliminary test. Therefore exact values of EC50 could not be calculated. The NOEC and EC100 values are identified by direct observation.
- Validity criteria fulfilled:
- yes
- Remarks:
- There was no immobilisation in the controls and the dissolved oxygen content remained above 3 mg/L.
- Conclusions:
- Under the conditions of this study, the 48 hour EC50 was determined to be >100 mg/L and the 48 hour NOEC was 100 mg/L.
- Executive summary:
The test substance, Solvent Red 19E, was investigated for acute toxicity to Daphnia magna under GLP conditions.
The test was performed according to method C.2 – Daphnia sp. Acute Immobilisation Test, Council Regulation (EC) No. 440/2008, published in O.J. L 142, 2008 and OECD Test Guideline No 202, Daphnia sp., Acute Immobilisation Test, Adopted: April 13, 2004.
In a preliminary study, 20 daphnids per concentration were exposed the test material at nominal concentrations of 10, 20, 40, 60 and 100 mg/L under static conditions.
The nominal concentrations were used for all evaluations and results. The study was performed without analytical determination of test substance concentrations because no suitable analytical method was found.
Since the test substance is poorly soluble in dilution water at the concentrations needed for the test, saturated solutions of the test substance in dilution water were prepared by adding measured amounts of the test substance to the dilution water. The saturated solutions were stirred for a period of 72 hours on a shaft stirrer and consequently filtered through 0.45 μm filter.
As no toxicity of the test substance was found in preliminary test, the study was terminated. The concentration of 100 mg/L included in the preliminary test was performed using 20 daphnids per group; therefore the conditions of the limit test have been fulfilled. It was demonstrated that the EC50 is higher than this concentration, thus no further testing is required.
Under the conditions of this study, the 48 hour EC50 was determined to be >100 mg/L and the 48 hour NOEC was 100 mg/L. The 48 hour EC100 was >100 mg/L.
Reference
Description of key information
The 48 hour EC50 was determined to be >100 mg/L and the 48 hour NOEC was 100 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
The test substance, Solvent Red 19E, was investigated for acute toxicity to Daphnia magna under GLP conditions.
The test was performed according to method C.2 – Daphnia sp. Acute Immobilisation Test, Council Regulation (EC) No. 440/2008, published in O.J. L 142, 2008 and OECD Test Guideline No 202, Daphnia sp., Acute Immobilisation Test, Adopted: April 13, 2004.
In a preliminary study, 20 daphnids per concentration were exposed the test material at nominal concentrations of 10, 20, 40, 60 and 100 mg/L under static conditions.
The nominal concentrations were used for all evaluations and results. The study was performed without analytical determination of test substance concentrations because no suitable analytical method was found.
Since the test substance is poorly soluble in dilution water at the concentrations needed for the test, saturated solutions of the test substance in dilution water were prepared by adding measured amounts of the test substance to the dilution water. The saturated solutions were stirred for a period of 72 hours on a shaft stirrer and consequently filtered through 0.45 μm filter.
As no toxicity of the test substance was found in preliminary test, the study was terminated. The concentration of 100 mg/L included in the preliminary test was performed using 20 daphnids per group; therefore the conditions of the limit test have been fulfilled. It was demonstrated that the EC50 is higher than this concentration, thus no further testing is required.
Under the conditions of this study, the 48 hour EC50 was determined to be >100 mg/L and the 48 hour NOEC was 100 mg/L. The 48 hour EC100 was >100 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.