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EC number: 244-691-9 | CAS number: 21981-33-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study in accordance to OECD GL
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 1,1,1,3,3-pentachlorobutane
- EC Number:
- 244-691-9
- EC Name:
- 1,1,1,3,3-pentachlorobutane
- Cas Number:
- 21981-33-9
- Molecular formula:
- C4H5Cl5
- IUPAC Name:
- 1,1,1,3,3-pentachlorobutane
- Details on test material:
- - Name of test material (as cited in study report): pentachlorobutane
- Molecular formula (if other than submission substance): same as submission substance
- Molecular weight (if other than submission substance): same as submission substance
- Smiles notation (if other than submission substance): same as submission substance
- Structural formula attached as image file (if other than submission substance): see Fig.
- Substance type: organic
- Physical state: liquid
- Analytical purity: 90%
- Impurities (identity and concentrations):
- Purity test date: 09/09/99
- Lot/batch No.: 99350L08
- Expiration date of the lot/batch: 13/09/2000
- Stability under test conditions: stable
- Storage condition of test material: at room temeprature in the dark
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Stock solution was prepared by adding 36 mg test substance to 0.5 g Chromosorb G (60-80 mesh) in a headspace vial to get a homogeneous solution in water. The vial was placed on a roller bank in a climate chamber at 20°C for 1 hour. Afterward, the content was transferred into a 320 ml flask completely filled with Elendt medium and closed with a screw cap and stored at room temperature for 3 days. The stock solution concentration was determined on the day of the test initiation to be 41.4 mg/l. The test solutions were prepared by consecutive dilutions.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water fleas
- Strain: Daphnia magna
- Source: Daphtoxkit F. magna (Bio International , the Netherlands)
- Age at study initiation (mean and range, SD): 0-24 h
- Weight at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD): not reported
- Method of breeding: cultured in Elendt M4 medium in a climate chamber (20°C). 16 h light/day.
- Feeding during test
- Food type: Chlorella vulgaris
- Amount and frequency not stated
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- Standard Elent M4 medium
- Test temperature:
- 20.4-21.2
- pH:
- 7.9 at day 0, 7.5-7.6 at day 2
- Dissolved oxygen:
- 8.4 mg/l at day 0, 8.5-8.6 at day 2
- Salinity:
- Standard Elent M4 medium
- Nominal and measured concentrations:
- - nominal concentrations: 0, 0.2, 0.4, 0.8, 1.6 and 3.2 mg/l
- measured concentrations (mean value): 0 (n.d.), 0.07, 0.26, 0.58, 1.3, 2.4 mg/l - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: Glass, no headspace, 320 ml
- Aeration: No
- Type of flow-through (e.g. peristaltic or proportional diluter): n/a
- Renewal rate of test solution (frequency/flow rate): n/a
- No. of organisms per vessel: 10-12
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ultrapure water prepared by reversed osmosis, followed by anion, cation and mixed bed chromatography and filtered through 0.22 um cellulose filter
- Conductivity: < 4 uS/cm
- Culture medium different from test medium: No - Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- ca. 1.8 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% confidence interval: 1.3-2.4 mg/l
- Details on results:
- - Behavioural abnormalities: 2 organisms lying on the bottom of the flask were observed at the NOEC (1.3 mg/l)
- Observations on body length and weight: n/a
- Other biological observations: no
- Mortality of control: No mortality or immobility observed
- Other adverse effects control: No
- Abnormal responses: No
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No
Any other information on results incl. tables
No immobility was observed in the control samples and up to 1.3 mg/l (measured conc.). 100% immobility was observed in the 3 samples at 2.4 mg/l (measured concentrations). A 48 hours EC50 was calculated by the geometric mean of the highest concentration without effect and the lowest concentrations with 100% immobility leading to a 48 -h EC50 of 1.8 mg/l.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The substance is classifiable as toxic to Daphnia
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