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EC number: 274-778-7 | CAS number: 70693-62-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2000-10-18 - 2001-03-21
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Details on sampling:
- Not applicable
- Vehicle:
- no
- Details on test solutions:
- not indicated
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Preparation of inoculum for exposure: the activated sludge was filtered trough dried and preweighed Whatman GFC filter papers which were then dried again at approximately 105°C for at least one hour, allowed to cool in a desiccator and reweighed. The mixed liquor suspended solids (MLSS) content of the activated sludge was then calculated. Synthetic sewage was added to the activated sludge and the mixture aerated overnight.
- Pretreatment: no further information is given.
- Initial biomass concentration: before test start, the MLSS content was determined and adjusted to 4 g/L by addition of dechlorinated tap water. - Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- not indicated
- Hardness:
- not indicated
- Test temperature:
- 18.1-19.1°C
- pH:
- 7.5-8.2
- Dissolved oxygen:
- not indicated
- Salinity:
- not indicated
- Nominal and measured concentrations:
- not indicated
- Details on test conditions:
- TEST SYSTEM
- Test vessel: BOD flasks
- Aeration: permanent aeration during incubation time with a Pasteur pipette connected to a laboratory supply of oil-free compressed air.
- No. of culture flasks per concentration (replicates): 1 at 1 and 10 mg/L ,3 at 100 mg/L
- No. of vessels per control (replicates): 2
- Suspended solids concentration: 4.0 g/L mixed liquor suspended solids
EFFECT PARAMETERS MEASURED : Oxygen measurement
TEST CONCENTRATIONS:
1, 10, 100 mg/L - Reference substance (positive control):
- yes
- Remarks:
- 3,5- Dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 9.5 mg/L
- Conc. based on:
- other: reference substance
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 8.0 - 11.2 mg/L
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- not indicated
- Results with reference substance (positive control):
- The EC50 of the reference substance was determined to be 9.5 mg/L; this is in the accepted range of 5 to 30 mg/L for 3,5- Dichlorophenol.
- Reported statistics and error estimates:
- not indicated
- Validity criteria fulfilled:
- yes
- Conclusions:
- The validity criteria required by the OECD Guideline No. 209 can be considered as fulfilled.
The specific respiration rate of the control performed at the end of the test series was found to be 24.2 mg O2/g*h. and was 97% of the respiration rate in the control performed at test start (25.0 mg O2/g*h). These results comply with the validity criteria of the test.
The EC50 of the reference substance was determined to be 9.5 mg/L; which is in the accepted range of 5 to 30 mg/L for 3,5- Dichlorophenol.
Oxone Monopersulfate Compound had no biologically significant effect on the respiration rate of activated sludge at any of the concentrations employed. - Executive summary:
Materials and methods
The test was conducted according to OECD Guideline No 209. Activated sludge from Eye Sewage Treatment Works for the treatment of domestic sewage was used.
EC20 >100 mg /L
EC50 >100 mg /L
EC80 >100 mg /L
Reference
O2 concentrations, respiration and inhibition rates of the test and the reference substance
Test mixture (mg/L) | Respiration rate (mg O2/g/h) | % inhibition |
Control 1 | 25.0 | - |
Test substance |
| |
1 | 26.8 | 0 |
10 | 26.3 | 0 |
100 | 21.4 | 13 |
100 | 21.4 | 13 |
100 | 21.7 | 12 |
3,5-DCP |
| |
3 | 22.1 | 10 |
10 | 10.6 | 57 |
32 | 3.8 | 85 |
Control 2 | 24.2 | - |
Description of key information
For the determination of the toxicity to microorganisms a key and a supporting study are available. In the supporting study the toxicity of KMPS triple salt to the gram negative bacterium Pseudomonas putida was determined in a growth inhibition test, according to the guideline of the German Umweltbundesamt: "Bewertung wassergefährdender Stoffe: Bestimmung der akuten Bakterientoxizität". The key study was conducted according to OECD Guideline 209.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 179 mg/L
- EC10 or NOEC for microorganisms:
- 108 mg/L
Additional information
Key study
The test was conducted according to OECD Guideline No 209. Activated sludge from Eye Sewage Treatment Works for the treatment of domestic sewage was used. The validity criteria required by the OECD Guideline No. 209 can be considered as fulfilled. The specific respiration rate of the control performed at the end of the test series was found to be 24.2 mg O2/g*h. and was 97% of the respiration rate in the control performed at test start (25.0 mg O2/g*h). These results comply with the validity criteria of the test. The EC50 of the reference substance was determined to be 9.5 mg/L; which is in the accepted range of 5 to 30 mg/L for 3,5- Dichlorophenol. Oxone Monopersulfate Compound had no biologically significant effect on the respiration rate of activated sludge at any of the concentrations employed.
EC20 >100 mg /L
EC50 >100 mg /L
EC80 >100 mg /L
Supporting study
In the supporting study the toxicity of KMPS triple salt to the gram negative bacterium Pseudomonas putida was determined in a growth inhibition test, according to the guideline of the German Umweltbundesamt: "Bewertung wassergefährdender Stoffe: Bestimmung der akuten Bakterientoxizität". The toxicity threshold, defined as 10% inhibition was determined to be EC10 = 108 mg KMPS triple salt/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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