Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 220-668-9 | CAS number: 2855-27-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1992
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
- Remarks:
- The study used an in-house method, but is an GLP study and is sufficiently documented.
- Qualifier:
- no guideline followed
- Qualifier:
- according to guideline
- Guideline:
- other: O2 consumption method with Pseudomonas putida
- Deviations:
- no
- Principles of method if other than guideline:
- Method: O2 consumption test with Pseudomonas putida, developed by Hüls AG. The principle of the method is to determine the consumption of oxygen of Pseudomonas putida by measurement of the oxygen content in closed test vessels with test medium, bacteria and different concentration of the test substance during incubation time (5.5 hours). The dose-response of the test substance on oxygen consumption is determined by comparison of the oxygen consumption rate between the control and the test vessel with the substance. The principle is comparable to the method according to guideline OECD209, with the difference that a pure culture of Pseudomonas putida is tested instead of a mixture of organisms in activated sludge.
- GLP compliance:
- yes
- Analytical monitoring:
- not required
- Details on sampling:
- - Concentrations: 0.1 mL/L; 0.5 mL/L; 1 mL/L; and 2 mL/L were tested
- Sampling method: at the end of incubation time HgCl2 (end concentration 3 mg/L) was added to the test vessels to stopp the reaction. Subsequently the oxygen concentration was measured. - Vehicle:
- yes
- Remarks:
- Emulsifier
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test substance was directly added to the test vessels with test medium in different concentrations. The test substance was dispersed in the test medium by addition of 1mL of the emulsifier nonylphenolethoxypropoxylate (stock solution 10 mL emulsifier in 190 mL water) into the test medium in the test vessels. After that the innoculate culture medium was added.
- Controls: test medium without test substance and test medium without test substance with HgCl2
- Chemical name of vehicle: nonylphenolethoxypropoxylate
- Concentration of vehicle in test medium: 1mL emulsifier per test vessel (stock solution 10 mL emulsifier in 190 mL water)
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): not specified. An interaction of the emulsifier and the test substance cannot be completely excluded. - Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- - Laboratory culture: Test organism Pseudomonas putida
- Source of the strain: Intitute for water, soil, and air hygiene (Berlin), afterwards stock culture in the laboratory
- Method of cultivation: Cultivation in test medium (stock solution I (contains buffer, glucose and trace elements) and II (contains iron sulfate and magnesium sulfate)
- Preparation of inoculum for exposure: liquid preculture, 19 h incubation, cell count 10 x 10^5 cells/mL
- Initial biomass concentration: 80 mL inoculum with 10 x 10^5 cells/mL - Test type:
- static
- Water media type:
- other: test medium
- Limit test:
- no
- Total exposure duration:
- 5.5 h
- Remarks on exposure duration:
- At the end of exposure, oxygen consumption was stopped by addition of 1mL HgCl2-solution to each test vessel.
- Test temperature:
- 25 +/- 2 °C
Preculture 24.9-25.1 °C, Incubation 24.0-26.8 °C - Nominal and measured concentrations:
- 0.1 mL/L; 0.5 mL/L; 1 mL/L and 2 mL/L (nominal) were tested
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL BSB-flask with magnetic stirrer
- Type: closed
- Material, size, headspace, fill volume: glass, 100 mL, closed with ground glass joint
- Aeration: no
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 5 (1 replicate is used to determine test end)
- No. of vessels per abiotic control (replicates): 4
- Nutrients provided for bacteria: glucose (in stock solution I)
- Incubation for 5.5 h at 25 °C +/-2 °C agitated in an incubator - Key result
- Duration:
- 5.5 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.89 other: mL/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks on result:
- other: Corresponding to an EC10 of 754 mg/L.
- Details on results:
- The EC10 value was determined two times based on 4 replicates. The first test results in an EC10 of 0.97 mL/L, the second test resulted in an EC10 of 0.80 mL/L. The mean value of both tests is an EC10 of 0.89 mL/L.The EC10 is the concentration were 10% inhibition of the oxygen consumption in comparison to the control was observed.
- Reported statistics and error estimates:
- The EC10 was calculated based on linear regression and probit analysis with the program Multiplan
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The effect of the test substance on Pseudomonas putida has been examined in an oxygen consumption test. An EC10 has been determined to 0.89 mL/L for 1,2,4-Trivinylcyclohexane in the oxygen consumption test.
Reference
Description of key information
An EC10 of 0.89 mL/L has been determined in an oxygen consumption test with Pseudomonas putida, corresponding to an EC10 of 754 mg/L of 1,2,4-Trivinylcyclohexane.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 754 mg/L
Additional information
The inhibition of the oxygen respiration rate of Pseudomonas putida by 1,2,4 -Trivinylcyclohexane has been examined in an oxygen consumption test by an in house developed method. In the dose-response test, the effect of the test substance on the oxygen consumption of Pseudomonas putida after 5.5 h has been tested. An EC10 of 0.89 mL/L has been determined, corresponding to an EC10 of 754 mg/L of 1,2,4-Trivinylcyclohexane in the oxygen consumption test.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.