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EC number: 700-408-5 | CAS number: 103429-90-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 27 August 2007 - 31 August 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Gudance Document 23 "Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures" (September 2000)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 35.0, 45.5, 59.2, 76.9 and 100 mg/L
- Sampling method: The test solutions for analysis were taken out from the middle layer in the test vessels. The concentration of the test item and 3-methoxy-3-methylbutan-1-ol was analyzed by GC. The concentration of acetic acid was analyzed by HPLC. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Desired amount of test sample and dilution water in the test vessels were mixed and stirred to prepare the test solutions.
- Controls: Only the dilution water was used as control. - Test organisms (species):
- Oryzias latipes
- Details on test organisms:
- TEST ORGANISM
- Common name: Medaka
- Source: Laboratory
- Age at study initiation: 5 months
- Length at study initiation (length definition, mean, range and SD): 2.0 +/- 1.0 cm
ACCLIMATION
- Acclimation period: 34 days
- Acclimation conditions (same as test or not): flow-through condition under the same water quality and temperature conditions.
- Type and amount of food during acclimation: feed mixture for carp, not fed for 25h before the start of exposure.
- Health during acclimation (any mortality observed): 0% mortality observed during the 7 days before the start of exposure.
FEEDING DURING TEST
- Food type: Test organisms were not fed during the exposure. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 37.0 mg/L
- Test temperature:
- 24 +/- 1 °C
- pH:
- 7.7 (at 24 °C), 7.6 - 7.9 during the test
- Dissolved oxygen:
- > 60 %
- Salinity:
- no data
- Conductivity:
- 15.5 mS/m
- Nominal and measured concentrations:
- Nominal: 35.0, 45.5, 59.2, 76.9 and 100 mg/L
Measured (test start): 36.4, 45.3, 60.6, 82.1, 109 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass tanks
- Type (delete if not applicable): glass cover
- Material, size, headspace, fill volume: 7.8 L glass tank (diameter: 24 cm, depth: 28 cm)
- Aeration: No
- Renewal rate of test solution (frequency/flow rate): renewal every 24h
- No. of organisms per vessel: 8 fish/test level
- No. of vessels per concentration (replicates): One replicate/test level
- No. of vessels per control (replicates): One replicate/test level (control)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated tap water
- Total organic carbon: < 0.1 mg/L
- Ca/mg ratio: 3.7
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 h / 8 h
- Light intensity: Artificial light of white fluorescent lamp
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Mortality and visible abnormality were observed at 3, 24, 48, 72 and 96 hours after the start of the test in each exposure level.
TEST CONCENTRATIONS
- Range finding study : Yes
- Test concentrations: Not known
- Results used to determine the conditions for the definitive study: Yes - Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 84.9 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: LC50 value estimated by the binomial test.
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 73.8 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: LC50 value estimated by the binomial test.
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 59.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks:
- No behavioural anomalies seen at this level, either.
- Details on results:
- - Behavioural abnormalities: One surviving fish dosed at 76.9 mg/L was seen swimming at the surface, was found to have a comlpete loss of equilibrium, and reduced activity after 72 hours.
- Mortality of control: No mortality was seen in the control group. - Reported statistics and error estimates:
- Binominal test
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 96-hour LC50 value in Medaka (Oryzias latipes) for the test item was determined to be 73.8 mg/L. The highest concentration (NOEC) showing no mortality after 96 hours was 59.2 mg/L.
- Executive summary:
An acute fish toxicity test was conducted to assess the effect of the test material on fish. The study was conducted according to OECD 203 and in compliance with GLP.
Test groups of eight Medaka (Oryzias latipes) per group were exposed to test medium containing the test material at concentrations of 0 (control), 35.0, 45.5, 59.2, 76.9, and 100 mg/L. The test was conducted over 96h, and the test medium was replaced at 24h-intervals. The fish were observed for mortality and abnormal behavious at 3h from the start of the exposure period, and then at 24, 48, 72, and 96h from the start of exposure. Concentrations of the test material and its hydrolysis products 3-methoxy-3-methylbutan-1-ol and acetic acid were verified by GC-FID and HPLC. Mortalities were seen in the 76.9 and 100 mg/L dose levels; all fish at the 100 mg/mL level had died by the 48h timepoint. The 96h LC50 value in Medaka (Oryzias latipes) for the test item was determined to be 73.8 mg/L. The highest concentration (NOEC) showing no mortality after 96h was 59.2 mg/L.
Reference
Description of key information
The 96h LC50 value in Medaka (Oryzias latipes) for the test item was determined to be 73.8 mg/L. The highest concentration (NOEC) showing no mortality after 96h was 59.2 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 73.8 mg/L
Additional information
An acute fish toxicity test was conducted to assess the effect of the test material on fish. The study was conducted according to OECD 203 and in compliance with GLP.
Test groups of eight Medaka (Oryzias latipes) per group were exposed to test medium containing the test material at concentrations of 0 (control), 35.0, 45.5, 59.2, 76.9, and 100 mg/L. The test was conducted over 96h, and the test medium was replaced at 24h-intervals. The fish were observed for mortality and abnormal behavious at 3h from the start of the exposure period, and then at 24, 48, 72, and 96h from the start of exposure. Concentrations of the test material and its hydrolysis products 3-methoxy-3-methylbutan-1-ol and acetic acid were verified by GC-FID and HPLC. Mortalities were seen in the 76.9 and 100 mg/L dose levels; all fish at the 100 mg/mL level had died by the 48h timepoint. The 96h LC50 value in Medaka (Oryzias latipes) for the test item was determined to be 73.8 mg/L. The highest concentration (NOEC) showing no mortality after 96h was 59.2 mg/L.
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