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EC number: 248-502-0 | CAS number: 27503-81-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to reproduction: other studies
Administrative data
- Endpoint:
- toxicity to reproduction: other studies
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- April 2002
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: scientifically valid method was used and study is adequately reported
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- The aim of this in vitro investigation was to evaluate a potential affinity of test article sodium salt for the androgen receptor (AR). The experimental system employed was capable of detecting strongly and weakly binding compounds such as dihydrotestosterone and androstenedione.
ln principle, the method described by Boesel and Shain (A rapid, specific protocol for determination of available androgen receptor sites in unfractioned rat ventral prostate cytosol preparations. Biochem Biophys Res Comm 61: 1004-1011, 1974) was applied, however, a rat recombinant fusion protein containing both the hinge region and ligand binding domain of the AR served as receptor source, whereas radiolabeled methyltrienolone was used as receptor ligand according to Kelce et al. (Kelce WR, Monosson E, Gamcsik MP, Laws SC, Gray LE jr., Environmental hormone disruptors: Evidence that vinclozolin developmental toxicity is mediated by antiandrogenic metabolites. Toxicol Appl Pharmacol 126: 276-285, 1994). - GLP compliance:
- yes (incl. QA statement)
- Type of method:
- in vitro
Test material
- Reference substance name:
- 2-phenyl-1H-benzimidazole-5-sulphonic acid
- EC Number:
- 248-502-0
- EC Name:
- 2-phenyl-1H-benzimidazole-5-sulphonic acid
- Cas Number:
- 27503-81-7
- Molecular formula:
- C13H10N2O3S
- IUPAC Name:
- 2-phenyl-1H-benzimidazole-5-sulphonic acid
Constituent 1
Test animals
- Details on test animals or test system and environmental conditions:
- Biological material:
Androgen receptor (AR): rat recombinant fusion protein to thioredoxin containing both the hinge region and ligand binding domain of the AR (PanVera, Madison, Wl, USA).
Equipment:
Labofuge A (Heraeus, Osterode, FRG)
Liquid scintillation counter 1900 TR (Canberra-Packard, Frankfurt, FRG)
Liquid scintillation counter 1450 Microbeta™ Trilux (Wallac, Freiburg, FRG)
Chemicals:
Tris(hydroxymethyl)-aminomethane (Tris) p.a. (E. Merck, Darmstadt, FRG)
Sodium chloride (KMF, St. Augustin, FRG)
Dithiothreitol (DTT ) (Sigma, Deisenhofen, FRG)
Glycerol anhydrous pure (E. Merck, Darmstadt, FRG)
Human y-globulin 96 - 99 % (Sigma, Deisenhofen, FRG)
Dimethylsulfoxid p.a. (DMSO) (E. Merck, Darmstadt, FRG)
Ethanol p.a. (E. Merck, Darmstadt, FRG)
Ultima Gold™ scintillation cocktail (Canberra-Packard, Frankfurt, FRG)
Ultima Flo AP™ scintillation cocktail (Canberra-Packard, Frankfurt, FRG)
Radiochemicals:
Methyltrienolone, [ 17a-Methyl -3 H] (R 1881) 3089.5 GBq/mmol (NEN Du Pont, Dreieich, FRG)
Fine chemicals, reference:
Dextrane coated charcoal (Sigma, Deisenhofen, FRG)
Methyltrienolone (R 1881) (NEN Du Pont, Dreieich, FRG)
4-Androstene-3,17-dione 98 % (Sigma, Steinheim, FRG)
Dihydrotestosterone (DHT) >99 % (Sigma, Steinheim, FRG)
Administration / exposure
- Details on exposure:
- Test compound was incubated overnight with 2 nM androgen receptor and 2 nM radiolabeled methyltrienolone. After removal of unbound ligand by adsorption to charcoal and centrifugation, receptor-bound methyltrienolone was determined by liquid scintillation counting. Incubations were performed in triplicate (control: n = 6 / coefficient of variation =3 %). Receptor binding in the presence of excess (5 µM) unlabeled R1881, i.e., unspecific binding was substracted. Ligand bound in the presence of test compound was related to ligand bound in the absence of compound and expressed as percentage of control.
- Analytical verification of doses or concentrations:
- no
- Duration of treatment / exposure:
- overnight
Doses / concentrations
- Dose / conc.:
- 1 other: mmol
- Remarks:
- Basis:
analytical conc.
test article sodium salt
- Control animals:
- other: dihydrotestosterone (DHT) and androstenedione (ANDRO)
- Details on study design:
- In order to screen for a potential affinity of test article sodium salt for the androgen receptor (AR), potential AR binding of test article sodium salt was investigated by means of a classical receptor binding assay using a rat recombinant fusion protein containing both the hinge region and ligand binding domain of the AR as receptor source and radiolabeled methyltrienolone as ligand. The physiological androgens dihydrotestosterone and androstenedione served as reference compounds with strong and weak affinity for the AR, respectively.
- Statistics:
- no data
Results and discussion
Effect levels
- Key result
- Dose descriptor:
- other:
- Basis for effect level:
- other: No binding to the androgen receptor at the maximally employable concentration of 1 mM.
- Remarks on result:
- not measured/tested
Any other information on results incl. tables
An affinity of test article sodium salt for the androgen receptor (AR) at the maximally employable concentration of one millimolar could not be demonstrated in two independent experiments. Within the experimental error, the quantity of radiolabeled ligand methyltrienolone bound in the presence of the test compound was comparable to that of the control. The reference compounds dihydrotestosterone (DHT) and androstenedione (ANDRO) reproducibly displaced radiolabeled methyltrienolone from the AR with IC50-values of 4.7 nM and 4.6 nM for DHT and 2.4 pM and 2.0 pM for ANDRO, respectively. In the presence of test article sodium salt generally no displacement of ligand was observed. A slight drop in ligand binding at the maximally testable concentration of 1 mM in one experiment representing the edge of the experimental error, was indistinguishable from that observed in the presence of 1 µM in the same experiment, and thus was not confirmed in the other experiment.
Applicant's summary and conclusion
- Conclusions:
- Under the conditions of this assay system, test article sodium salt was found to have no affinity for the androgen receptor (AR).
- Executive summary:
This study was performed to investigate the potential affinity of test article for the androgen receptor (AR) by means of a classical receptor binding assay using a rat recombinant fusion protein containing both the hinge region and ligand binding domain of the AR as receptor source and radiolabeled methyltrienolone as ligand.
Whereas reference compounds dihydrotestosterone and androstenedione reproducibly displaced radiolabeled methyltrienolone from the AR, an affinity of test article sodium salt for the AR at the maximally employable concentration of one mmol could not be demonstrated.
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