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EC number: 219-842-7 | CAS number: 2550-02-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Please refer to the justification for grouping of substances provided in IUCLID section 13.
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 80 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 58 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Conclusions:
- A 96-hour LC50 value of 80 mg/L and NOEC of 58 mg/L have been determined for the effects of the test substance on mortality of Brachydanio rerio.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1994-01-10 to 1994-01-14
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Test substance concentrations were measured in fresh and old test media in which there were surviving at the start of the test and then after 24, 48 and 72 hours exposure periods.
- Vehicle:
- no
- Details on test solutions:
- A stock solution of the test substance was prepared at a nominal concentration of 1000 mg/L by mixing for 18 hours followed by filtration. Treatments were prepared by dilution of the stock solution.
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebrafish
- Source: West aquarium, Bad Lauterberg, Germany
- Length at study initiation (length definition, mean, range and SD): 3.0 cm +/-0.5 cm
- Feeding during test: none
ACCLIMATION
- Acclimation period: at least 14 days
- Acclimation conditions (same as test or not): yes
- Type and amount of food: TetraMin® at 1% of body weight
- Feeding frequency: Daily
- Health during acclimation (any mortality observed): The fish exhibit normal behaviour and are free from visible signs of disease at the start of the test. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 12.8 ºdH
- Test temperature:
- 20 - 21ºC
- pH:
- 7.4 - 8.3
- Dissolved oxygen:
- 0.4 - 8.9 mg/L
Low dissolved oxygen concentrations were only recorded in test vessels containing dead fish. Measured dissolved concentrations in treatments (34, 58 and 94 mg/L) where there was 100% fish survival over at least some 24 hour exposure periods were always in the range 5.8-8.5 mg/L. Judging from the measured dissolved oxygen concentrations prior to fish deaths, the low concentrations are therefore not considered to be a cause of the fish deaths but rather a consequence of the presence of the dead fish. - Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal concentrations: 0 (Control) and 6, 10, 17, 28 and 50% dilutions of the stock solution.
Corresponding geometric mean measured concentrations were: 0 (Control), 34, 58, 94, 173 and 299 mg/L. - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: open aquarium
- Material, size, headspace, fill volume: 30 litre
- Aeration: Continuous
- Renewal rate of test solution (frequency/flow rate): Daily
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.4 g/l
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Potable water from Gelsenwasser AG
OTHER TEST CONDITIONS
- Adjustment of pH: None
- Photoperiod: 16 h light/8 h dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality after 24, 48, 72 and 96 h - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 80 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 58 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Mortality of control: 0
- Reported statistics and error estimates:
- The methods of determining the LC50 and LC0 (NOEC) values are not reported. However a dose-response curve is presented showing the LC50 value.
- Sublethal observations / clinical signs:
Table 1. Results of analysis of test media.
Nominal treatment (% stock solution)
Measured concentration at start of test (mg/L)
Measured concentration in old/fresh media at 24 hours
Measured concentration in old/fresh media at 48 hours
Measured concentration in old/fresh media at 72 hours
Measured concentration in old media at 96 hours
Geometric mean measured concentration (mg/L)
0 (Control)
-
-
-
-
-
-
6
40
31/36
28/41
31/41
25
34
10
79
47/53
57/64
46/70
51
58
17
121
89/94
74/106
81/112
79
94
28
191
157/-
-/-
-/-
-
173
50
329
272/-
-/-
-/-
-
299
Table 2. Test results
Nominal treatment (% stock solution)
Cumulative mortality after 24 hours (%)
Cumulative mortality after 48 hours (%)
Cumulative mortality after 72 hours (%)
Cumulative mortality after 96 hours (%)
0 (Control)
0
0
0
0
6
0
0
0
0
10
0
0
10
10
17
0
50
50
70
28
100
100
100
100
50
100
100
100
100
- Conclusions:
- A 96-hour LC50 value of 80 mg/L and NOEC of 58 mg/L have been determined for the effects of the test substance on mortality of Brachydanio rerio.
Referenceopen allclose all
Description of key information
LC50(96h): 80 mg/l (Brachydanio rerio, EU method C.1, read across from CAS 5089-70-3)
Key value for chemical safety assessment
Additional information
Note: In order to support the read across from (3-chloropropyl) triethoxysilane (CAS 5089-70-3) to triethoxy(propyl)silane (CAS 2550-02-9) an OECD 203 is currently ongoing. The dossier will be updated accordingly once the final report is available.
Measured data were not available for triethoxy(propyl)silane (CAS 2550-02-9). It was considered appropriate to read across from the structurally analogous substance, (3-chloropropyl) triethoxysilane (5089-70-3).
A 96-hour LC50value of 80 mg/l and NOEC of 58 mg/l have been determined for the effects of the test substance on mortality of Brachydanio rerio based on a guideline study (EU method C.1 acute toxicity to fish, Hüls 1995).
The results are interpreted with reference to mean measured concentrations of the test substance.
Additional good quality data for the analogue substance, trimethoxy(propyl)silane (CAS 1067-25-0), have been read across for triethoxy(propyl)silane. Both substances share the same silanol hydrolysis product, propylsilanetriol; the other hydrolysis products being ethanol and methanol. A 96 hr LC50 of >746 mg/l (EU Method C.1 (Acute Toxicity for Fish) was determined for the effects of trimethoxy(propyl)silane (CAS 1067-25-0) on mortality of Brachydanio rerio based on mean measured concentrations.
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