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EC number: 427-720-1 | CAS number: 26364-65-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study according to OECD guideline
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
- Report date:
- 1997
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- acute toxic class method
- Limit test:
- no
Test material
- Reference substance name:
- -
- EC Number:
- 427-720-1
- EC Name:
- -
- Cas Number:
- 26364-65-8
- Molecular formula:
- C4H5N3S
- IUPAC Name:
- [(1,3-thiazolidin-2-ylidene)amino]formonitrile
- Details on test material:
- name: Cyanamide, (4,5-dihydroxy-2-thiazolyl)-
molecular formula: C4 H5 N3 S
molecular weight: 127.2
physical state: solid
analytical purity: 97.3 %
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Harlan Winkelmann GmbH, Borchen, Germany
- Age at study initiation: 6 to 8 weeks
- Weight at study initiation: male animals 174 -180 g and the female animals of 169 - 187 g.
- Fasting period before study: approx. 17 hours + 1 hour
- Housing: polycarbonate cages type III
- Diet: ad libitum
- Water: e.g. ad libitum
- Acclimation period: 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22° + 1,5°C
- Humidity (%): 40% - 70%
- Photoperiod (hrs dark / hrs light): approx. 12-hour artificial lighting from 6 a.m. to 6 p.m.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- CMC (carboxymethyl cellulose)
- Doses:
- 2000 mg/kg bw and 200 mg/kg bw.
- No. of animals per sex per dose:
- 3 animals each. Females at 2000 mg/kg bw and 200 mg/kg bw. Males at 200 mg/kg bw.
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: several times on the day of treatment, and at least once a day thereafter. The body weights of the rats are recorded on day 1 before administration and then weekly. Additionally, all animals that died or are sacrificed are weighed.
- Necropsy of survivors performed: yes
Results and discussion
Effect levels
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- > 300 - < 500 mg/kg bw
- Mortality:
- All animals of the 2000 gm/kg bw dose group died.
- Clinical signs:
- other: At the dose of 200 mg/kg body weight in both sexes piloerection, decreased motility and labored breathing were observed. Females of the 200 mg/kg group and 2000 mg/kg group showed decreased reactivity, spasmodic state, increased salivation, narrowed palpe
- Gross pathology:
- in animals that died during the observation period the following changes were detected:
liver: dark-red discoloration
glandular stomach: red discoloration
lung: slightly collapsed
kidneys: pale
No gross pathologic changes were observed in animals sacrificed at the end of the study period.
Applicant's summary and conclusion
- Interpretation of results:
- harmful
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The substance is to be classified as harmful according to the results of this study.
- Executive summary:
A study for acute oral toxicity in male and female Wistar rats was conducted with the
test substance CIT (2-Cyanimino-1,3-thiazolidin) according to OECD Guideline 423.
A dose of 200 mg/kg body weight was tolerated by male and female rats without
mortalities.
At the dose of 200 mg/kg body weight in both sexes piloerection, decreased motility
and labored breathing were observed. Females of the 200 mg/kg and 2000 mg/kg
groups showed decreased reactivity, spasmodic state, increased salivation,
narrowed palpebral fissure and temporary labored breathing. Additionally, in 200
mg/kg females spastic gait and in 2000 mg/kg females lateral position, tonical
cramps, temporary tremor, temporary accelerated breathing and red salivation
occurred.
There were no toxicological effects on body weights or on body weight development
in males. In 200 mg/kg group females the body weight development was slightly
retarded.
The gross pathology investigations performed at the end of the post-observation
period did not afford any treatment-related findings.
According to the „Interpretation of results", for the test substance investigated a
LD50 of > 300 < 500 mg/kg b.w. was determined.
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