Propanenitrile, 2,3,3,3-tetrafluoro-2-(trifluoromethyl)-

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

May 21 to July 10, 2020

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch number of test material: 3M N.B # us278898.001-096
- Expiration date of the lot/batch: 10/30/2021
- Purity: 97.7%
- Physical state: White solid

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature
- Stability under storage conditions: Stable

Analytical monitoring:

yes

Details on sampling:

- Concentrations: Range finding test: 1.2, 12, 120 mg/L. Limit test: 120 mg/L
- 20 mL analytical samples were taken from each vessel at the beginning and end of the test, and before and after each renewal.
- Sample storage conditions before analysis: Samples were stored and refrigerated at 2-8° C if not analyzed within 24 hours

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION:
- Method: The test substance was dissolved in test medium to prepare a stock solution with a nominal concentration of 1000 mg/L. The test solutions were then prepared daily via dilution of the stock solution with test water.
- Controls: Blank control of test water.

Test organisms (species):

other: Gobiocypris rarus

Details on test organisms:

TEST ORGANISM
- Common name: Chinese Rare Minnow
- Source: Bioassay and Safety Assessment Lab, Shanghai Academy of Public Measurement
- Length at study initiation: 1.7 ± 0.1cm (Mean ± SD) for fish from the same batch (n=10)
- Weight at study initiation: 0.10 ± 0.01g (Mean ± SD) for fish from the same batch (n=10)

ACCLIMATION
- Acclimation period: More than 9 days using test conditions.
- Type and amount of food during acclimation: Fish were fed with brine shrimp flakes (OSI, USA) daily until 24 hours before test.
- Health during acclimation (any mortality observed): No visible signs of disease, or malformations. Fish appeared stress free and were not treated for diseases nor parasites during the acclimation period. Less than 5% mortality in the population was observed before the test.
-Acclimation conditiions: Water: standatd dilution water, Temperature: 21-25 °C, pH: 6.0-8.5, Dissolved Oxygen: >80% air saturation value (ASV), Light: 12-16 hours per day.

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

145 mg CaCO3/L

Test temperature:

21.3 - 21.6 °C

pH:

6.74 - 7.68

Dissolved oxygen:

71.2 - 93.8 % ASV

Nominal and measured concentrations:

Nominal: 120 mg/L.
Measured: A time-weighted average of 127 mg/L was calculated for 120 mg/L concentration.

Details on test conditions:

TEST SYSTEM
- Test vessel: Five liter glass vessels with seals.
- Type: closed
- Material, size, headspace, fill volume: No headspace
- Aeration: Yes
- Renewal rate of test solution: Every 24 hours
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.2 g fish/L

TEST MEDIUM / WATER PARAMETERS
- Total organic carbon: 112.7 ug/L
- Salts were dissolved in deionized water (conductivity:≤10µs/cm) to form stock solutions and standard dilution water:
Stock a: Component CaCl2.2H2O, concentration 11.76 g/L
Stock b: Component MgSO4.7H2O, concentration 4.93 g/L
Stock c: Component KCl, concentration 0.23 g/L
Stock d: NaHCO3, concentration 2.59 g/L
Standard dilution water: 25mL of each solution (a-d) were mixed to the total volume of 1 liter with deionized water, which was then aereated over 24 hours.

OTHER TEST CONDITIONS
- Adjustment of pH: None
- Photoperiod: 12 hours dark: 12 hours light
- Light intensity: 810 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Mortality and other effects (including visible physical and behavioral abnormalities) at 2, 5, 24, 28, 48, 52, 72, 76 and 96 hours.

TEST CONCENTRATIONS
- Range finding study : Yes
- Test concentrations: 1.2, 12, 120 mg/L
- Results used to determine the conditions for the definitive study: Yes, a limit test was run.

Reference substance (positive control):

yes

Remarks:

Potassium dichlorate

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 127 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other:

Remarks:

No effects observed on exposed fish.

Details on results:

Behavioural abnormalities: No
- Mortality of control: No
- Other adverse effects control: No
- Abnormal responses: No
- Effect concentrations exceeding solubility of substance in test medium: No

Results with reference substance (positive control):

96 hour LC50 = 182 mg/L (95% CI: 144-288 mg/L) for potassium dichromate on fish from the same lot

Validity criteria fulfilled:

yes

Remarks:

<10% mortality in controls (0%), DO >60% ASV (71.2 - 93.8 %)

Conclusions:

96 hour LC50 >127 mg/L (OECD 203) in Gobiocypris rarus.

Executive summary:

The 96 hour LC50 of Heptafluoropropylamide to Gobiocypris rarus was determined in a limit test according to OECD 203 guidelines. A nominal concentration of 120 mg/L and a blank control were run using 10 animals per tank. An LC50 >127 mg/L (time weighted average concentration) was determined. No mortality or adverse effects were observed at this concentration.

The study was well-documented, followed an international standard method and was GLP compliant.The study is considered reliable without restrictions.The results from this study are considered suitable for Risk Assessment, Classification and Labeling, and PBT Analysis.

Description of key information

96 hour LC50 >127 mg/L (OECD 203) in Gobiocypris rarus.

Additional information

The 96 hour LC50 of Heptafluoropropylamide to Gobiocypris rarus was determined in a limit test according to OECD 203 guidelines. A nominal concentration of 120 mg/L and a blank control were run using 10 animals per tank. An LC50 >127 mg/L (time weighted average concentration) was determined. No mortality or adverse effects were observed at this concentration.

The study was well-documented, followed an international standard method and was GLP compliant.The study is considered reliable without restrictions.The results from this study are considered suitable for Risk Assessment, Classification and Labeling, and PBT Analysis.