Tall oil, reaction products with diethylenetriamine

Toxicity to soil macroorganisms except arthropods

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

toxicity to soil macroorganisms except arthropods: long-term

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

It is considered appropriate to address the data requirements for DTO_DETA by read-across to the available studies on the main components of DTO_DETA: AAI_DETA and Rosin.
DTO_DETA and AAI_DETA are each a mixture of constituents which include monoamide, diamide, residual amine and imidazoline (mono-, di- and tri-condensate) chemical structures. The substances therefore have common functional groups based on amide, amine and imidazoline moieties and are sufficiently similar in terms of chemical structure to support a read-across approach.
DTO_DETA contains comparatively lower levels of imidazolines and higher levels of resin acids than AAI_DETA and therefore consideration of data for resin acids is also considered necessary. The main resin acid in DTO_DETA is abietic acid, but abietic acid derivatives and other acids, such as pimaric acid, are also found in notable quantities, and the resin acids collectively are known as ‘rosin’. DTO_DETA contains upto 25% unreacted rosin, and taking into account the compositional information available for the rosin in DTO_DETA and Rosin (CAS# 8050-09-07, EC# 232-475-7), the latter was considered appropriate for read-across to DTO_DETA.
The AAI_DETA dataset is worst case with regards to biodegradability and aquatic toxicity, with the dataset for Rosin showing a lower toxicity profile. Therefore, AAI_DETA is considered to be a worst case read-across source for the DTO_DETA target substance in the context of toxicity to soil macroorganisms.

Reason / purpose for cross-reference:

read-across source

Key result

Duration:

56 d

Dose descriptor:

NOEC

Effect conc.:

1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Duration:

56 d

Dose descriptor:

NOEC

Effect conc.:

944 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

reproduction

Details on results:

- Mortality at end of exposure period: After 28 days of exposure to the test item no noteworthy mortality of adult earthworms (≤ 5%) occurred in the control as well as at all test item concentrations
- Changes in body weigth of live adults (% of initial weight) at end of exposure period: At test start the earthworms had an individual weight of 0.30 - 0.60 g. The body weights of the earthworms increased in the control and at all test item concentrations throughout the first 28 days. The biomass increase was not statistically significant compared to the control at all test item concentrations
- No. of offspring produced: The mean number of juveniles in the control was 323. The average number of juveniles in the test item concentrations ranged between 265 and 347. Compared to the control the reproduction of the earthworms was not statistically significant at 125, 250, 500 and 1000 mg/kg soil dry weight. At 62.5 mg/kg soil dry weight the earthworm reproduction rate was statistically significantly reduced compared to the control.
- Behavioural abnormalities: No symptoms or changes in the behaviour of the earthworms were observed in the control as well as at all test item concentrations after 28 days of exposure to the test item

Results with reference substance (positive control):

- Results with reference substance valid? Yes
- Relevant effect levels: 1.0 mg Carbendazim/kg soil DW

Reported statistics and error estimates:

One Way Analysis of Variance (ANOVA) was carried out for the determination of statistically significant differences compared to the control. A Normality Test and an Equal Variance Test were conducted prior to running the ANOVA. P-values for both Normality and Equal Variance Test are 0.05. The a-value for ANOVA test (acceptable probability of incorrectly concluding that there is a difference) is a = 0.05.

Mortality of Adult Earthworms in [%] after 28 Days of Exposure

Application rate [mg/kg soil dry weight]	Mortality[%]
	Replicate								Mean
	1	2	3	4	5	6	7	8
Control	0	0	0	10	0	0	0	0	1.25
62.5	10	0	10	0	–				5.00
125	0	0	0	0					0.00
250	0	0	0	0					0.00
500	0	0	0	0					0.00
1000	0	0	0	0					0.00

Earthworm Behaviour and Pathological Symptoms after 28 Days of Exposure

Application rate [mg/kg soil dry weight]	Effect	Replicate
		1	2	3	4	5	6	7	8
Control	A)	10/10	10/10	10/10	9/10	10/10	10/10	10/10	10/10
	H)	0/10	0/10	0/10	1/10	0/10	0/10	0/10	0/10
62.5	A)	9/10	10/10	9/10	10/10	–
	H)	1/10	0/10	1/10	0/10
125	A)	10/10	10/10	10/10	10/10
250	A)	10/10	10/10	10/10	10/10
500	A)	10/10	10/10	10/10	10/10
1000	A)	10/10	10/10	10/10	10/10

Body Weight Changes of the Adult Earthworms      

Application rate [mg/kg soil dry weight]	Rep.	Mean body weights per replicate		Mean body weight change of earthworms
		Test start	28 days	Per replicate		Mean±SD		Sign.*
		[g]	[g]	[g]	[%]	[g]	[%]
Control	1	0.500	0.675	0.18	35.0	0.15 ± 0.03	28.8 ± 5.31	–
	2	0.531	0.645	0.11	21.5
	3	0.490	0.625	0.14	27.6
	4	0.526	0.704	0.18	33.9
	5	0.511	0.655	0.14	28.2
	6	0.500	0.662	0.16	32.4
	7	0.490	0.593	0.10	21.0
	8	0.506	0.661	0.16	30.6
62.5	1	0.531	0.710	0.18	33.7	0.18 ± 0.02	36.3 ± 3.42	No
	2	0.508	0.716	0.21	40.9
	3	0.491	0.671	0.18	36.7
	4	0.507	0.678	0.17	33.7
125	1	0.513	0.690	0.18	34.5	0.15 ± 0.03	29.9 ± 6.82	No
	2	0.487	0.614	0.13	26.1
	3	0.509	0.696	0.19	36.7
	4	0.528	0.646	0.12	22.3
250	1	0.528	0.671	0.14	27.1	0.16 ± 0.02	30.8 ± 4.72	No
	2	0.512	0.675	0.16	31.8
	3	0.494	0.677	0.18	37.0
	4	0.500	0.636	0.14	27.2
500	1	0.503	0.636	0.13	26.4	0.16 ± 0.06	31.2 ± 11.9	No
	2	0.491	0.712	0.22	45.0
	3	0.525	0.616	0.09	17.3
	4	0.509	0.692	0.18	36.0
1000	1	0.531	0.639	0.11	20.3	0.16 ± 0.04	31.8 ± 9.12	No
	2	0.504	0.719	0.22	42.7
	3	0.514	0.681	0.17	32.5
	4	0.488	0.643	0.16	31.8

Reproduction Rate (Number of Juveniles after 8 Weeks)

Application rate [mg/kg soil dry weight]	Rep.	Number of juveniles	Mean±SD	CV              [%]	[%] of control	Significance compared to control
Control	1	292	323 ± 27.2	8.43	–	–
	2	285
	3	332
	4	299
	5	353
	6	355
	7	332
	8	334
62.5	1	227	265 ± 39.4	14.9	82.0	Yes
	2	260
	3	252
	4	320
125	1	316	301 ± 26.1	8.65	93.3	No
	2	322
	3	302
	4	264
250	1	350	347 ± 41.3	11.9	107	No
	2	296
	3	397
	4	343
500	1	310	307 ± 8.04	2.62	95.1	No
	2	316
	3	305
	4	297
1000	1	259	291 ± 29.8	10.2	90.2	No
	2	281
	3	330
	4	295

Validity criteria fulfilled:

yes

Conclusions:

The toxicity of the target substance DTO_DETA to soil macroorganisms is estimated to be a NOEC = 944.0 mg a.i./kg soil dw, based on read-across from a study testing AAI_DETA.

Executive summary:

The toxicity of the target substance DTO_DETA to soil macroorganisms is estimated to be a NOEC = 944.0 mg a.i./kg soil dw, based on read-across from a study testing AAI_DETA.

As explained in the justification for type of information, DTO_DETA and AAI_DETA are each a mixture of constituents which include monoamide, diamide, residual amine and imidazoline (mono-, di- and tri-condensate) chemical structures. The substances therefore have common functional groups based on amide, amine and imidazoline moieties and are sufficiently similar in terms of chemical structure to support a read-across approach.

DTO_DETA contains comparatively lower levels of imidazolines and higher levels of resin acids than AAI_DETA and therefore consideration of data for resin acids is also considered necessary. The main resin acid in DTO_DETA is abietic acid, but abietic acid derivatives and other acids, such as pimaric acid, are also found in notable quantities, and the resin acids collectively are known as ‘rosin’. DTO_DETA contains upto 25% unreacted rosin, and taking into account the compositional information available for the rosin in DTO_DETA and Rosin (CAS# 8050-09-07, EC# 232-475-7), the latter was considered appropriate for read-across to DTO_DETA.

The AAI_DETA dataset is worst case with regards to biodegradability and aquatic toxicity, with the dataset for Rosin showing a lower toxicity profile. No data for the toxicity to soil macroorganisms are available for Rosin. Therefore, AAI_DETA is considered to be a worst case read-across source for the DTO_DETA target substance in the context of toxicity to soil macroorganisms.

Reference 2

Endpoint:

toxicity to soil macroorganisms except arthropods: long-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2010-01-22 to 2010-03-23

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study, GLP, All validity criteria fulfilled, complete identification of test substance

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

OECD Guideline 222 (Earthworm Reproduction Test (Eisenia fetida/Eisenia andrei))

Version / remarks:

DIN ISO 11268-2: 1998 (E), BBA Guideline (1994)

Deviations:

yes

Remarks:

Room temperature increased once for about four hours to 23 °C. . The soil moisture of all test vessels deviated by more than 10 % of the initial moisture at the end of the test. No pre-moistening of the natural soil.

GLP compliance:

yes

Specific details on test material used for the study:

- Name of test material (as cited in study report): Tall oil diethylenetriamine imidazoline (CAS No. 68442-97-7)
- Physical state: Liquid, amber, viscous
- Analytical purity: 94.4 %
- Purity test date: 2009-05-11
- Lot/batch No.: S-000922
- Expiration date of the lot/batch: 2017-07-02
- Water solubility: Insoluble
- Density: 980 kg/m3 at 20 °C
- pH: 10 - 12 at 75 % solution
- Storage condition of test material: Room temperature, protected from moisture and light, under nitrogen

Analytical monitoring:

no

Vehicle:

yes

Details on preparation and application of test substrate:

- Method of mixing into soil (if used): The test item was weighed out and was evenly distributed on 10 g quartz sand (vehicle) per replicate. The treated quartz sand was evenly distributed over the natural soil. Demineralised water was added to adjust the humidity of the natural soil to a moisture content of 56 % of the maximum water holding capacity.
Thereafter, the test medium was mixed for at least three minutes and about 600 g soil dry weight were filled into each test vessel.
- Controls: Natural soil with the addition of quartz sand (10 g pre replicate) without test or reference item.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): quartz sand
- Concentration of vehicle in test medium (stock solution and final test solution): 10 g per replicate
- Evaporation of vehicle before use: Not necessary

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

TEST ORGANISM
- Common name: Eisenia fetida SAVIGNY 1826, subspecies Eisenia fetida fetida
- Source: Own breeding
- Age at test initiation (mean and range, SD): synchronized earthworm culture, not deviating more than 1 month
- Weight at test initiation (mean and range, SD): 0.51 g


ACCLIMATION
- Acclimation period: 2 days prior to test start
- Acclimation conditions (same as test or not): same as test
- Health during acclimation (any mortality observed): healthy

Study type:

laboratory study

Substrate type:

natural soil

Limit test:

no

Total exposure duration:

56 d

Remarks:

Removement of adults after 28 days

Test temperature:

20 +/- 2°C

pH:

Application rate
[mg/kg soil dry weight] pH-value
day 0 day 56
Control 5.47 6.22
62.5 5.64 6.39
125 5.61 6.31
250 5.65 6.29
500 5.74 6.33
1000 5.95 6.28

Moisture:

Application rate
[mg/kg soil dry weight] Moisture [%] of dry weight

day 0 day 60
Control 25.5 29.3*
62.5 26.5 29.7*
125 26.6 29.5*
250 26.2 30.5*
500 25.5 28.4*
1000 24.9 29.1*

Details on test conditions:

TEST SYSTEM
- Test container (material, size): round plastic boxes, ID: 15 cm, bottom surface area: 177 cm3, height: 14 cm, transparent and perforated lids enabled sufficient gas exchange and light penetration and prevented the test substrate from drying
- Amount of soil or substrate: 600 g DW
- No. of organisms per container (treatment): 10 (40)
- No. of replicates per treatment group: 4
- No. of replicates per control: 8



SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
A loamy sand (LUFA 2.2) soil (field fresh sampled) was used in the study. The soil was received by the LANDWIRTSCHAFTLICHE UNTERSUCHUNGS- UND FORSCHUNGSANSTALT SPAYER, Obere Langgasse 40, 67346 Speyer, Germany. The soil was sampled at January 4th, 2010 and stored at 6 ± 2 °C in the test facility until use. The soil parameters, determined before the beginning of the definitive study, are listed in Table 3. The soil history (years 2005 to 2009) is given below:
Pesticides:
Sampling year and 4 former years: None

Plants:
2009: Meadow
2008: Meadow
2007: Meadow
2006: Meadow
2005: Meadow

Fertilization:
No organic fertilization
2009: None
2008: None
2007: 600 kg/ha CaO
+ 250 kg/ha MgO
2006: None
2005: None


- Composition (natural soil):
Parameter LUFA-soil 2.2
Batch-No. F 2.20210
pH-value* 5.5 ± 0.1
Soil moisture before application [%] 14.1
Maximum water capacity* [g/100 g soil dry weight] 46.5 ± 6.0
Particle size distribution acc. to DIN*
Sand:
0.63 – 2.0 mm (%) 0.7 ± 0.2
0.2 – 0.63 mm (%) 44.5 ± 2.6
0.063 – 0.2 mm (%) 35.5 ± 2.2
Silt:
0.02 – 0.063mm (%) 5.8 ± 1.1
0.006 – 0.02 mm (%) 4.0 ± 0.6
0.002 – 0.006mm (%) 3.0 ± 0.6
Clay:
< 0.002 mm (%) 6.4 ± 0.9

- Organic carbon (%): 2.09 +/- 0.4
- Maximum water holding capacity (in % dry weigth): 46.5
- CEC: 10.0 +/- 0.5 mVal/100 g
- Pretreatment of soil: none
- Storage (condition, duration): 14 days after receipt at test conditions in the dark



OTHER TEST CONDITIONS
- Photoperiod: 16 h light - 8 h dark
- Light intensity: 560 +/- 88 lx

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Adult mortality, biomass, behaviour and pathological symptoms after 28 days
Reproduction (Number of juveniles) after 56 days

VEHICLE CONTROL PERFORMED: No


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2

Nominal and measured concentrations:

Nominal application rates: 62.5 - 125 - 250 - 500 - 1000 mg test substance/kg soil dry weight (DW)

Reference substance (positive control):

yes

Remarks:

1.0 - 2.5 - 5.0 mg Carbendazim/ kg soil DW

Duration:

56 d

Dose descriptor:

NOEC

Effect conc.:

1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Duration:

56 d

Dose descriptor:

NOEC

Effect conc.:

944 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

reproduction

Details on results:

- Mortality at end of exposure period: After 28 days of exposure to the test item no noteworthy mortality of adult earthworms (≤ 5%) occurred in the control as well as at all test item concentrations
- Changes in body weigth of live adults (% of initial weight) at end of exposure period: At test start the earthworms had an individual weight of 0.30 - 0.60 g. The body weights of the earthworms increased in the control and at all test item concentrations throughout the first 28 days. The biomass increase did not differ statistically significant compared to the control at all test item concentrations
- No. of offspring produced: The mean number of juveniles in the control was 323. The average number of juveniles in the test item concentrations ranged between 265 and 347 (Table 8). Compared to the control the reproduction of the earthworms did not statistically significant differ at 125, 250, 500 and 1000 mg/kg soil dry weight. At 62.5 mg/kg soil dry weight the earthworm reproduction rate was statistically significantly reduced compared to the control.
- Behavioural abnormalities: No symptoms or changes in the behaviour of the earthworms were observed in the control as well as at all test item concentrations after 28 days of exposure to the test item

Results with reference substance (positive control):

- Results with reference substance valid? Yes
- Relevant effect levels: 1.0 mg Carbendazim/kg soil DW

Reported statistics and error estimates:

One Way Analysis of Variance (ANOVA) was carried out for the determination of statistically significant differences compared to
the control. A Normality Test and an Equal Variance Test were conducted prior to running the ANOVA. P-values for both Normality and Equal Variance Test are 0.05. The a-value for ANOVA test (acceptable probability of incorrectly concluding that there is a difference) is a = 0.05.

Mortality of Adult Earthworms in [%] after 28 Days of Exposure

Application rate [mg/kg soil dry weight]	Mortality [%]
	Replicate								Mean
	1	2	3	4	5	6	7	8
Control	0	0	0	10	0	0	0	0	1.25
62.5	10	0	10	0	–				5.00
125	0	0	0	0					0.00
250	0	0	0	0					0.00
500	0	0	0	0					0.00
1000	0	0	0	0					0.00

Earthworm Behaviour and Pathological Symptoms after 28 Days of Exposure

Application rate [mg/kg soil dry weight]	Effect	Replicate
		1	2	3	4	5	6	7	8
Control	A)	10/10	10/10	10/10	9/10	10/10	10/10	10/10	10/10
	H)	0/10	0/10	0/10	1/10	0/10	0/10	0/10	0/10
62.5	A)	9/10	10/10	9/10	10/10	–
	H)	1/10	0/10	1/10	0/10
125	A)	10/10	10/10	10/10	10/10
250	A)	10/10	10/10	10/10	10/10
500	A)	10/10	10/10	10/10	10/10
1000	A)	10/10	10/10	10/10	10/10

Body Weight Changes of the Adult Earthworms      

Application rate [mg/kg soil dry weight]	Rep.	Mean body weights per replicate		Mean body weight change of earthworms
		Test start	28 days	Per replicate		Mean ± SD		Sign.*
		[g]	[g]	[g]	[%]	[g]	[%]
Control	1	0.500	0.675	0.18	35.0	0.15 ± 0.03	28.8 ± 5.31	–
	2	0.531	0.645	0.11	21.5
	3	0.490	0.625	0.14	27.6
	4	0.526	0.704	0.18	33.9
	5	0.511	0.655	0.14	28.2
	6	0.500	0.662	0.16	32.4
	7	0.490	0.593	0.10	21.0
	8	0.506	0.661	0.16	30.6
62.5	1	0.531	0.710	0.18	33.7	0.18 ± 0.02	36.3 ± 3.42	No
	2	0.508	0.716	0.21	40.9
	3	0.491	0.671	0.18	36.7
	4	0.507	0.678	0.17	33.7
125	1	0.513	0.690	0.18	34.5	0.15 ± 0.03	29.9 ± 6.82	No
	2	0.487	0.614	0.13	26.1
	3	0.509	0.696	0.19	36.7
	4	0.528	0.646	0.12	22.3
250	1	0.528	0.671	0.14	27.1	0.16 ± 0.02	30.8 ± 4.72	No
	2	0.512	0.675	0.16	31.8
	3	0.494	0.677	0.18	37.0
	4	0.500	0.636	0.14	27.2
500	1	0.503	0.636	0.13	26.4	0.16 ± 0.06	31.2 ± 11.9	No
	2	0.491	0.712	0.22	45.0
	3	0.525	0.616	0.09	17.3
	4	0.509	0.692	0.18	36.0
1000	1	0.531	0.639	0.11	20.3	0.16 ± 0.04	31.8 ± 9.12	No
	2	0.504	0.719	0.22	42.7
	3	0.514	0.681	0.17	32.5
	4	0.488	0.643	0.16	31.8

Reproduction Rate (Number of Juveniles after 8 Weeks)

Application rate [mg/kg soil dry weight]	Rep.	Number of juveniles	Mean ± SD	CV               [%]	[%] of control	Significance compared to control
Control	1	292	323 ± 27.2	8.43	–	–
	2	285
	3	332
	4	299
	5	353
	6	355
	7	332
	8	334
62.5	1	227	265 ± 39.4	14.9	82.0	Yes
	2	260
	3	252
	4	320
125	1	316	301 ± 26.1	8.65	93.3	No
	2	322
	3	302
	4	264
250	1	350	347 ± 41.3	11.9	107	No
	2	296
	3	397
	4	343
500	1	310	307 ± 8.04	2.62	95.1	No
	2	316
	3	305
	4	297
1000	1	259	291 ± 29.8	10.2	90.2	No
	2	281
	3	330
	4	295

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of this study, Tall oil diethylenetriamine imidazoline did not induce evident mortality or pathological symptoms of adult earthworms after an exposure to the test item concentrations of 62.5, 125, 250, 500 and 1000 mg/kg soil dry weight for 28 days. The earthworm biomass increased in the control and at all tested application rates over 28 days without any statistically significant differences. After further four weeks the earthworm reproduction rate was not statistically significantly reduced compared to the control at 125, 250, 500 and 1000 mg/kg soil dry weight, whilst at the lowest concentration of 62.5 mg/kg soil dry weight a slight significant reduction was observed. Since this effect on earthworm reproduction only occurs at the lowest test item concentration, this effect is not considered to be a test item related effect (no dose response).

Thus, the No Observed Effect Concentration (NOEC) of Tall oil diethylenetriamine imidazoline with respect to mortality, body weight and reproduction of Eisenia fetida after 8 weeks of exposure in natural soil was determined to be 1000 mg/kg soil dry weight.

Executive summary:

Effects of Tall oil diethylenetriamine imidazoline (batch number S000922) on mortality, biomass and the reproductive potential of the earthworm species Eisenia fetida (Savigny) were determined according to the guidelines DIN ISO 11268-2 (1998), OECD 222 (2004) and BBA (1994) from January 22^nd to March 23^rd, 2010 with the definitive exposure phase from January 22^nd to March 19^th, 2010 at Dr.U.Noack-Laboratorien in 31157 Sarstedt, Germany. The study was conducted under static conditions over 8 weeks with five different application rates of 62.5 - 125 - 250 - 500 - 1000 mg/kg soil dry weight, which were mixed into natural soil. Eighty sexually mature earthworms were inserted into 8 control replicates (10 earthworms per replicate) and forty sexually mature earthworms were divided into 4 replicates for the test item concentrations. They had an individual body weight between 0.30 and 0.60 g.

After 28 days no biologically significant mortality was seen in the control and at all tested application rates (Table 1). Also, no pathological symptoms or changes in the behaviour of adult earthworms were seen in the control and at all tested application rates. The earthworm biomass increased in the control and at all tested application rates over 28 days without any statistically significant differences compared to the control. After further four weeks the reproduction rate (average number of juveniles) was 323 in the control and ranged between 265 and 347 at the test item application rates. The earthworm reproduction rate was not statistically significantly reduced compared to the control at 125, 250, 500 and 1000 mg/kg soil dry weight, whilst at the lowest concentration of 62.5 mg/kg soil dry weight a significant reduction was observed. Since this effect on earthworm reproduction only occurs at the lowest test item concentration, this effect is not considered to be a test item related effect (no dose response).

Overall, the NOEC of Tall oil diethylenetriamine imidazolineconcerning mortality, biomass and reproduction of earthworms was determined to be at 1000 mg/kg soil dry weight.

All validity criteria recommended by the test guidelines were fulfilled.

Summary of All Observed Effects in the Tested Doses

Effects	Tall oil diethylenetriamine imidazoline
mg/kg soil dry weight	62.5	125	250	500	1000
Mortality of adult earthworms	No	No	No	No	No
Reduction of body weight	No	No	No	No	No
Reduced reproduction rate   (Number of juveniles)	Yes*	No	No	No	No
Summary of all effects	No	No	No	No	No

*) = no test item related effect

NOEC and LOEC for Reproduction, Mortality and Body Weight

Tall oil diethylenetriamine imidazoline
	Application rate
	[mg/kg soil dry weight]
NOECreproduction, mortality, body weight	1000
LOECreproduction, mortality, body weight	> 1000

Description of key information

It is considered appropriate to address the data requirements for DTO_DETA by read-across to the available studies on the main components of DTO_DETA: AAI_DETA and Rosin.

The long-term earthworm study for AAI_DETA revealed a NOEC of 944.0 mg a.i./kg soil dw. No data were available for Rosin.

The AAI_DETA dataset is worst case with regards to biodegradability and aquatic toxicity, with the dataset for Rosin showing a lower toxicity profile. No data for the toxicity to soil macroorganisms are available for Rosin. Therefore, AAI_DETA is considered to be a worst case read-across source for the DTO_DETA target substance in the context of toxicity to soil macroorganisms. The toxicity of DTO_DETA to soil macroorganisms is therefore estimated to be a NOEC = 944.0 mg a.i./kg soil dw.

Key value for chemical safety assessment

Long-term EC10, LC10 or NOEC for soil macroorganisms:

944 mg/kg soil dw