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EC number: 215-325-5 | CAS number: 1321-74-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Data is from J-check
Data source
Reference
- Reference Type:
- review article or handbook
- Title:
- In Vitro Chromosomal Aberration Test of Divinylbenzene on Cultured Chinese Hamster C ells
- Author:
- J-check
- Year:
- 2 003
- Bibliographic source:
- Ministry of Health and Welfare, Japan, 2003
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- Guidelines for Screening Toxicity Testings of Chemicals (Japan) and OECD Guidelines No. 471 and 472
- Principles of method if other than guideline:
- Gene mutation test was conducted on Salmonella typhimurium TA 100, TA 1535, TA 98, TA 15371 and Escherichia coli WP 2 uvr A 2 by using test chemical
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Divinylbenzene
- EC Number:
- 215-325-5
- EC Name:
- Divinylbenzene
- Cas Number:
- 1321-74-0
- Molecular formula:
- C10H10
- IUPAC Name:
- 1,2-di(ethenyl)benzene
- Details on test material:
- - Name of test material: Divinylbenzene- IUPAC name: 1,2-di(ethenyl)benzene - Molecular formula: C10H10- Molecular weight: 131.1969 g/mol- Substance type: Organic- Physical state: No data - Purity: No data- Impurities (identity and concentrations): No data
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- not specified
- Species / strain / cell type:
- E. coli WP2 uvr A
- Details on mammalian cell type (if applicable):
- not specified
- Additional strain / cell type characteristics:
- not specified
- Cytokinesis block (if used):
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- Rat liver, induced with phenobarbital and 5,6-benzoflavone
- Test concentrations with justification for top dose:
- -S9 mix; 0, 0.781, 1.56, 3.13, 6.25, 12.5, 25.0, 50.0 μg/plate (TA1535(Test 1)); 0, 1.56 - 50.0 μg/plate(TA1535(Test 2)); 0, 3.13 - 100 μg/plate(TA100, TA98,TA1537); 0, 6.25 - 200 μg/plate(WP2 uvrA)+S9 mix; 0, 6.25 - 200 μg/plate(TA100, TA1 535, TA98, TA1537); 0, 15.6 - 500 μg/plate(WP2 uvrA)
- Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- sodium azide
- other: 2- (2-furyl) -3- (5-nitro-2-furyl) acrylamide and 2-aminoanthracene
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in medium; preincubationDURATION- Preincubation period: 20 minutes- Exposure duration: 48 hoursNUMBER OF REPLICATIONS: 2 METHODS OF SLIDE PREPARATION AND STAINING TECHNIQUE USED: 0.1 mL of the test substance preparation solution, 0.5 mL of the phosphate buffer solution (0.5 mL of the S 9 mix in the S9 mix addition test) and 0.1 mL of the test bacterium solution were mixed in a small test tube and preincubated at 37 ° C. for 20 minutes , Add 2 mL of top agar and mix and sonicate it on a synthetic medium flat plate.
- Evaluation criteria:
- Among the five test bacteria used, in the S9 mix-free test or S9 mix addition test of one or more test bacteria, the average value of the number of mutant colonies on the flat plate containing the test substance was 2 Fold or more, and when reproducibility and dose dependency were observed in the increase, the test substance was judged to be mutagenic (positive) in this test system.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium, other: TA100, TA1535, TA98, TA1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Remarks on result:
- other: Non mutagenic
Applicant's summary and conclusion
- Conclusions:
- The test chemical did not induce gene mutation in Salmonella typhimurium TA100, TA1535, TA98, TA1537, Escherichia coli WP2 uvrA - in the presence and absence of rat liver activation system and hence the chemical is not likely to classify as a gene mutant in vitro.
- Executive summary:
Gene mutation toxicity study was performed to determine the mutagenic nature of the test chemical. The study was performed using TA100, TA1535, TA98, TA1537, Escherichia coli WP2 uvrA with and without Rat liver, induced with phenobarbital and 5,6-benzoflavone activation system. 0.1 mL of the test substance preparation solution, 0.5 mL of the phosphate buffer solution (0.5 mL of the S 9 mix in the S9 mix addition test) and 0.1 mL of the test bacterium solution were mixed in a small test tube and preincubated at 37 ° C. for 20 minutes, Add 2 mL of top agar and mix and sonicate it on a synthetic medium flat plate. As a control group, a solvent to be used or several kinds of positive control substance solutions was used in place of the test substance preparation solution. The names and dosages of positive control substances used for each test bacterium are shown in each Table. Solvents and positive control groups were shared with other tests performed simultaneously. Cultivation was carried out at 37 ° C. for 48 hours. Toxicity was observed at 50.0 μg/plate (TA100, TA1535, TA1537) and 100 μg/plate(TA98, WP2 uvrA) without an S9 mix, and at 100 μg/plate(TA100, TA1535, TA98, TA1537) and 250 μg/plate(WP2 uvrA) with an S9 mix. The test chemical did not induce gene mutation in Salmonella typhimurium TA100, TA1535, TA98, TA1537, Escherichia coli WP2 uvrA - in the presence and absence of rat liver activation system and hence the chemical is not likely to classify as a gene mutant in vitro.
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