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EC number: 239-044-2 | CAS number: 14970-87-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Not reported.
- Vehicle:
- no
- Details on test solutions:
- The test item is very soluble and a stock solution of 100 mg/L was easily prepared on the day of the start of the trial.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea (Daphnia magna Straus)
- Strain/clone: Clone 5
- Bred in Elendt M7 and fed with Raphidocelis subcapitata
- Selection of test organisms (< 24h) by sieving
- Feeding during test: None
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- None.
- Post exposure observation period:
- No post-exposure observation.
- Hardness:
- Not measured.
- Test temperature:
- 19.5-20.5°C (min-max, as measured in the experimental room)
- pH:
- 7.9-8.2 (min-max)
- Dissolved oxygen:
- 8.5-9.2 mg/L (min-max)
- Salinity:
- Freshwater
- Conductivity:
- Not measured.
- Nominal and measured concentrations:
- Nominal: 0 ; 0.1 ; 0.2 ; 0.4 ; 0.8 ; 1.6 ; 3.1 ; 6.25 ; 12.5 ; 25 ; 50 ; 100 mg/L
Measured at 0h:Measured at 72h:
The four highest concentrations did not deviate by more than 20% from the initial measured concentrations. LD was 6.4 mg/L and it was therefore not surprising that expected concentrations close to or lower than 6.4 mg/L could not be detected. All in all, it was considered reasonable to use nominal concentrations to calculate the adverse effects of DMDO on Daphnia in this study. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 120 mL flask closely tight with aluminium caps equipped with PTFE septa.
- Renewal: static test, no headspace.
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS: as described in ISO 6341 standard
OTHER TEST CONDITIONS
- Adjustment of pH: None
- Photoperiod: Darkness
- Light intensity: Not measured
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobility as defined in the OECD 202 guideline. - Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.76 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Details on results:
- Immobility of the daphnids is presented in table 1. 48h-EC50 was 0.76 mg/L
- Results with reference substance (positive control):
- 24h-EC50 with potassium dichromate was 0.85 mg/L for mobility.
- Reported statistics and error estimates:
- Statistical analysis in the report was found to be erroneous. Reanalysis of the data was performed with ToxRat 3.2.2 software. The statistical output is attached to the RSS.
- Validity criteria fulfilled:
- yes
- Conclusions:
- 48h-EC50 was 0.76 mg/L.
- Executive summary:
The acute toxicity (inhibition of mobility) of dimercapto-1,8 -dioxa-3,6 -octane (DMDO) for Daphnia magna was assessed according to the method C2 of the European Directive 92/69/CEE. The study was carried out in compliance with the Principles of OECD Good Laboratory Practices.
Daphnia were exposed in a static test to a concentration range of 0.1 to 100 mg/L DMDO (spacing factor 2). The test was performed with 5 daphnids per vessel. Since DMDO is volatile, the test was performed using closed flasks as test glassware. In order to avoid volatilisation of DMDO, flasks were entirely filled with test solutions and closed with butyl rubber caps covered with PTFE.
For each exposure concentration, the percentage of immobilisation at 24 hours and 48 hours was recorded. The test concentrations of DMDO were measured by GC. 48h-EC50 was 0.76 mg/L. All validity criteria were fulfilled.
Reference
Table 1: Immobilisation of Daphnia magna in response to graded concentrations of DMDO (mg/L).
|
IMMOBILITY |
|||||
Nominal concentration |
Flask 1 |
Flask 2 |
Flask 3 |
Flask 4 |
Total |
% Immob. |
Control |
0 |
0 |
0 |
0 |
0 |
0 |
0.1 |
0 |
0 |
0 |
0 |
0 |
0 |
0.2 |
1 |
0 |
0 |
0 |
1 |
5 |
0.4 |
1 |
1 |
0 |
0 |
2 |
10 |
0.8 |
2 |
1 |
0 |
0 |
3 |
15 |
1.6 |
3 |
1 |
1 |
1 |
6 |
30 |
3.1 |
1 |
0 |
0 |
0 |
0 |
5 |
6.3 |
1 |
1 |
1 |
0 |
3 |
15 |
12.5 |
4 |
1 |
1 |
1 |
7 |
35 |
25 |
3 |
2 |
1 |
1 |
7 |
35 |
50 |
5 |
4 |
3 |
2 |
14 |
70 |
100 |
5 |
5 |
4 |
3 |
17 |
85 |
Description of key information
The acute toxicity (inhibition of mobility) of dimercapto-1,8 -dioxa-3,6 -octane (DMDO) for Daphnia magna was assessed according to the method C2 of the European Directive 92/69/CEE. The study was carried out in compliance with the Principles of OECD Good Laboratory Practices.
Daphnia were exposed in a static test to a concentration range of 0.1 to 100 mg/L DMDO (spacing factor 2). The test was performed with 5 daphnids per vessel. Since DMDO is volatile, the test was performed using closed flasks as test glassware. In order to avoid volatilisation of DMDO, flasks were entirely filled with test solutions and closed with butyl rubber caps covered with PTFE.
For each exposure concentration, the percentage of immobilisation at 24 hours and 48 hours was recorded. The test concentrations of DMDO were measured by GC. 48h-EC50 was 0.76 mg/L. All validity criteria were fulfilled.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.76 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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